Antigens of worms and eggs showed a differentiated detection of specific IgG according to the time of Schistosoma mansoni infection in mice
INTRODUCTION: The correlation between the immunological assay and the antibody titer can offer a tool for the experimental analysis of different phases of the disease. METHODS: Two simple immunological assays for Schistosoma mansoni in mice sera samples based on specific IgG detection for worms solu...
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ftdoajarticles:oai:doaj.org/article:00c1ba16fe004fa2ab719603f10f4244 2023-05-15T15:08:14+02:00 Antigens of worms and eggs showed a differentiated detection of specific IgG according to the time of Schistosoma mansoni infection in mice Rafaella Fortini Queiroz Grenfell Watson Hermann Martins Vanessa Silva-Moraes Suedali Villas-Boas Barata Elizandra Giani Ribeiro Edward Oliveira Paulo Marcos Zech Coelho 2012-08-01T00:00:00Z https://doi.org/10.1590/S0037-86822012000400018 https://doaj.org/article/00c1ba16fe004fa2ab719603f10f4244 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000400018&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822012000400018 https://doaj.org/article/00c1ba16fe004fa2ab719603f10f4244 Revista da Sociedade Brasileira de Medicina Tropical, Vol 45, Iss 4, Pp 505-509 (2012) Esquistossomose mansônica IgG. Antígenos de vermes Fases aguda Arctic medicine. Tropical medicine RC955-962 article 2012 ftdoajarticles https://doi.org/10.1590/S0037-86822012000400018 2022-12-31T03:01:13Z INTRODUCTION: The correlation between the immunological assay and the antibody titer can offer a tool for the experimental analysis of different phases of the disease. METHODS: Two simple immunological assays for Schistosoma mansoni in mice sera samples based on specific IgG detection for worms soluble antigens and eggs soluble antigens were standardized and evaluated in our laboratory. Fifty mice were used in negative and positive groups and the results obtained by enzyme-linked immunosorbent assays (ELISA) assays were compared with the number of worms counted and the IgG titers at different times of infection. RESULTS: Data showed that ELISA using adult worm antigens (ELISA-SWAP) presented a satisfactory correlation between the absorbance value of IgG titers and the individual number of worms counted after perfusion technique (R²=0.62). In addition, ELISA-SWAP differentially detected positive samples with 30 and 60 days post infection (p=0.011 and 0.003, respectively), whereas ELISA using egg antigens (ELISA-SEA) detected samples after 140 days (p=0.03). CONCLUSIONS: These data show that the use of different antigens in immunological methods can be used as potential tools for the analysis of the chronological evolution of S. mansoni infection in murine schistosomiasis. Correlations with human schistosomiasis are discussed. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Aguda ENVELOPE(-26.750,-26.750,-76.150,-76.150) Arctic Revista da Sociedade Brasileira de Medicina Tropical 45 4 505 509 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
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English |
topic |
Esquistossomose mansônica IgG. Antígenos de vermes Fases aguda Arctic medicine. Tropical medicine RC955-962 |
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Esquistossomose mansônica IgG. Antígenos de vermes Fases aguda Arctic medicine. Tropical medicine RC955-962 Rafaella Fortini Queiroz Grenfell Watson Hermann Martins Vanessa Silva-Moraes Suedali Villas-Boas Barata Elizandra Giani Ribeiro Edward Oliveira Paulo Marcos Zech Coelho Antigens of worms and eggs showed a differentiated detection of specific IgG according to the time of Schistosoma mansoni infection in mice |
topic_facet |
Esquistossomose mansônica IgG. Antígenos de vermes Fases aguda Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: The correlation between the immunological assay and the antibody titer can offer a tool for the experimental analysis of different phases of the disease. METHODS: Two simple immunological assays for Schistosoma mansoni in mice sera samples based on specific IgG detection for worms soluble antigens and eggs soluble antigens were standardized and evaluated in our laboratory. Fifty mice were used in negative and positive groups and the results obtained by enzyme-linked immunosorbent assays (ELISA) assays were compared with the number of worms counted and the IgG titers at different times of infection. RESULTS: Data showed that ELISA using adult worm antigens (ELISA-SWAP) presented a satisfactory correlation between the absorbance value of IgG titers and the individual number of worms counted after perfusion technique (R²=0.62). In addition, ELISA-SWAP differentially detected positive samples with 30 and 60 days post infection (p=0.011 and 0.003, respectively), whereas ELISA using egg antigens (ELISA-SEA) detected samples after 140 days (p=0.03). CONCLUSIONS: These data show that the use of different antigens in immunological methods can be used as potential tools for the analysis of the chronological evolution of S. mansoni infection in murine schistosomiasis. Correlations with human schistosomiasis are discussed. |
format |
Article in Journal/Newspaper |
author |
Rafaella Fortini Queiroz Grenfell Watson Hermann Martins Vanessa Silva-Moraes Suedali Villas-Boas Barata Elizandra Giani Ribeiro Edward Oliveira Paulo Marcos Zech Coelho |
author_facet |
Rafaella Fortini Queiroz Grenfell Watson Hermann Martins Vanessa Silva-Moraes Suedali Villas-Boas Barata Elizandra Giani Ribeiro Edward Oliveira Paulo Marcos Zech Coelho |
author_sort |
Rafaella Fortini Queiroz Grenfell |
title |
Antigens of worms and eggs showed a differentiated detection of specific IgG according to the time of Schistosoma mansoni infection in mice |
title_short |
Antigens of worms and eggs showed a differentiated detection of specific IgG according to the time of Schistosoma mansoni infection in mice |
title_full |
Antigens of worms and eggs showed a differentiated detection of specific IgG according to the time of Schistosoma mansoni infection in mice |
title_fullStr |
Antigens of worms and eggs showed a differentiated detection of specific IgG according to the time of Schistosoma mansoni infection in mice |
title_full_unstemmed |
Antigens of worms and eggs showed a differentiated detection of specific IgG according to the time of Schistosoma mansoni infection in mice |
title_sort |
antigens of worms and eggs showed a differentiated detection of specific igg according to the time of schistosoma mansoni infection in mice |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2012 |
url |
https://doi.org/10.1590/S0037-86822012000400018 https://doaj.org/article/00c1ba16fe004fa2ab719603f10f4244 |
long_lat |
ENVELOPE(-26.750,-26.750,-76.150,-76.150) |
geographic |
Aguda Arctic |
geographic_facet |
Aguda Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 45, Iss 4, Pp 505-509 (2012) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000400018&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822012000400018 https://doaj.org/article/00c1ba16fe004fa2ab719603f10f4244 |
op_doi |
https://doi.org/10.1590/S0037-86822012000400018 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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45 |
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4 |
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505 |
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509 |
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