In vitro action of antiparasitic drugs, especially artesunate, against Toxoplasma gondii
INTRODUCTION: Toxoplasmosis is usually a benign infection, except in the event of ocular, central nervous system (CNS), or congenital disease and particularly when the patient is immunocompromised. Treatment consists of drugs that frequently cause adverse effects; thus, newer, more effective drugs a...
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ftdoajarticles:oai:doaj.org/article:009e0e8ea0734080aad6f0be8375689b 2023-05-15T15:11:15+02:00 In vitro action of antiparasitic drugs, especially artesunate, against Toxoplasma gondii Thaís Cobellis Gomes Heitor Franco de Andrade Júnior Susana Angélica Zevallos Lescano Vicente Amato-Neto 2012-08-01T00:00:00Z https://doi.org/10.1590/S0037-86822012000400014 https://doaj.org/article/009e0e8ea0734080aad6f0be8375689b EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000400014&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822012000400014 https://doaj.org/article/009e0e8ea0734080aad6f0be8375689b Revista da Sociedade Brasileira de Medicina Tropical, Vol 45, Iss 4, Pp 485-490 (2012) Artesunato Tratamento Atividade anti-Toxoplasma Toxicidade Arctic medicine. Tropical medicine RC955-962 article 2012 ftdoajarticles https://doi.org/10.1590/S0037-86822012000400014 2022-12-30T23:18:08Z INTRODUCTION: Toxoplasmosis is usually a benign infection, except in the event of ocular, central nervous system (CNS), or congenital disease and particularly when the patient is immunocompromised. Treatment consists of drugs that frequently cause adverse effects; thus, newer, more effective drugs are needed. In this study, the possible activity of artesunate, a drug successfully being used for the treatment of malaria, on Toxoplasma gondii growth in cell culture is evaluated and compared with the action of drugs that are already being used against this parasite. METHODS: LLC-MK2 cells were cultivated in RPMI medium, kept in disposable plastic bottles, and incubated at 36ºC with 5% CO2. Tachyzoites of the RH strain were used. The following drugs were tested: artesunate, cotrimoxazole, pentamidine, pyrimethamine, quinine, and trimethoprim. The effects of these drugs on tachyzoites and LLC-MK2 cells were analyzed using nonlinear regression analysis with Prism 3.0 software. RESULTS: Artesunate showed a mean tachyzoite inhibitory concentration (IC50) of 0.075µM and an LLC MK2 toxicity of 2.003µM. Pyrimethamine was effective at an IC50 of 0.482µM and a toxicity of 11.178µM. Trimethoprim alone was effective against the in vitro parasite. Cotrimoxazole also was effective against the parasite but at higher concentrations than those observed for artesunate and pyrimethamine. Pentamidine and quinine had no inhibitory effect over tachyzoites. CONCLUSIONS: Artesunate is proven in vitro to be a useful alternative for the treatment of toxoplasmosis, implying a subsequent in vivo effect and suggesting the mechanism of this drug against the parasite. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 45 4 485 490 |
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English |
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Artesunato Tratamento Atividade anti-Toxoplasma Toxicidade Arctic medicine. Tropical medicine RC955-962 |
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Artesunato Tratamento Atividade anti-Toxoplasma Toxicidade Arctic medicine. Tropical medicine RC955-962 Thaís Cobellis Gomes Heitor Franco de Andrade Júnior Susana Angélica Zevallos Lescano Vicente Amato-Neto In vitro action of antiparasitic drugs, especially artesunate, against Toxoplasma gondii |
topic_facet |
Artesunato Tratamento Atividade anti-Toxoplasma Toxicidade Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: Toxoplasmosis is usually a benign infection, except in the event of ocular, central nervous system (CNS), or congenital disease and particularly when the patient is immunocompromised. Treatment consists of drugs that frequently cause adverse effects; thus, newer, more effective drugs are needed. In this study, the possible activity of artesunate, a drug successfully being used for the treatment of malaria, on Toxoplasma gondii growth in cell culture is evaluated and compared with the action of drugs that are already being used against this parasite. METHODS: LLC-MK2 cells were cultivated in RPMI medium, kept in disposable plastic bottles, and incubated at 36ºC with 5% CO2. Tachyzoites of the RH strain were used. The following drugs were tested: artesunate, cotrimoxazole, pentamidine, pyrimethamine, quinine, and trimethoprim. The effects of these drugs on tachyzoites and LLC-MK2 cells were analyzed using nonlinear regression analysis with Prism 3.0 software. RESULTS: Artesunate showed a mean tachyzoite inhibitory concentration (IC50) of 0.075µM and an LLC MK2 toxicity of 2.003µM. Pyrimethamine was effective at an IC50 of 0.482µM and a toxicity of 11.178µM. Trimethoprim alone was effective against the in vitro parasite. Cotrimoxazole also was effective against the parasite but at higher concentrations than those observed for artesunate and pyrimethamine. Pentamidine and quinine had no inhibitory effect over tachyzoites. CONCLUSIONS: Artesunate is proven in vitro to be a useful alternative for the treatment of toxoplasmosis, implying a subsequent in vivo effect and suggesting the mechanism of this drug against the parasite. |
format |
Article in Journal/Newspaper |
author |
Thaís Cobellis Gomes Heitor Franco de Andrade Júnior Susana Angélica Zevallos Lescano Vicente Amato-Neto |
author_facet |
Thaís Cobellis Gomes Heitor Franco de Andrade Júnior Susana Angélica Zevallos Lescano Vicente Amato-Neto |
author_sort |
Thaís Cobellis Gomes |
title |
In vitro action of antiparasitic drugs, especially artesunate, against Toxoplasma gondii |
title_short |
In vitro action of antiparasitic drugs, especially artesunate, against Toxoplasma gondii |
title_full |
In vitro action of antiparasitic drugs, especially artesunate, against Toxoplasma gondii |
title_fullStr |
In vitro action of antiparasitic drugs, especially artesunate, against Toxoplasma gondii |
title_full_unstemmed |
In vitro action of antiparasitic drugs, especially artesunate, against Toxoplasma gondii |
title_sort |
in vitro action of antiparasitic drugs, especially artesunate, against toxoplasma gondii |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2012 |
url |
https://doi.org/10.1590/S0037-86822012000400014 https://doaj.org/article/009e0e8ea0734080aad6f0be8375689b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 45, Iss 4, Pp 485-490 (2012) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000400014&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822012000400014 https://doaj.org/article/009e0e8ea0734080aad6f0be8375689b |
op_doi |
https://doi.org/10.1590/S0037-86822012000400014 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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45 |
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4 |
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485 |
op_container_end_page |
490 |
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