Head-to-head comparison of two loop-mediated isothermal amplification (LAMP) kits for diagnosis of malaria in a non-endemic setting
Abstract Background Light microscopy and rapid diagnostic tests (RDT) have long been the recommended diagnostic methods for malaria. However, in recent years, loop-mediated isothermal amplification (LAMP) techniques have been shown to offer superior performance, in particular concerning low-grade pa...
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ftdoajarticles:oai:doaj.org/article:009af99799d84253ad3af989d0c75b7d 2024-01-21T10:04:08+01:00 Head-to-head comparison of two loop-mediated isothermal amplification (LAMP) kits for diagnosis of malaria in a non-endemic setting Anna-Clara Ivarsson Elin Fransén Ioanna Broumou Anna Färnert Kristina E. M. Persson Sara Karlsson Söbirk 2023-12-01T00:00:00Z https://doi.org/10.1186/s12936-023-04809-7 https://doaj.org/article/009af99799d84253ad3af989d0c75b7d EN eng BMC https://doi.org/10.1186/s12936-023-04809-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-023-04809-7 1475-2875 https://doaj.org/article/009af99799d84253ad3af989d0c75b7d Malaria Journal, Vol 22, Iss 1, Pp 1-10 (2023) Malaria LAMP Loop-mediated isothermal amplification Diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2023 ftdoajarticles https://doi.org/10.1186/s12936-023-04809-7 2023-12-24T01:47:20Z Abstract Background Light microscopy and rapid diagnostic tests (RDT) have long been the recommended diagnostic methods for malaria. However, in recent years, loop-mediated isothermal amplification (LAMP) techniques have been shown to offer superior performance, in particular concerning low-grade parasitaemia, by delivering higher sensitivity and specificity with low laboratory capacity requirements in little more than an hour. In this study, the diagnostic performance of two LAMP kits were assessed head-to-head, compared to highly sensitive quantitative real time PCR (qPCR), in a non-endemic setting. Methods In this retrospective validation study two LAMP kits; Alethia® Illumigene Malaria kit and HumaTurb Loopamp™ Malaria Pan Detection (PDT) kit, were evaluated head-to-head for detection of Plasmodium-DNA in 133 biobanked blood samples from suspected malaria cases at the Clinical Microbiology Laboratory of Region Skåne, Sweden to determine their diagnostic performance compared to qPCR. Results Of the 133 samples tested, qPCR detected Plasmodium DNA in 41 samples (defined as true positives), and the two LAMP methods detected 41 and 37 of those, respectively. The results from the HumaTurb Loopamp™ Malaria PDT kit were in complete congruence with the qPCR, with a sensitivity of 100% (95% CI 91.40–100%) and specificity of 100% (95% CI 96.07–100%). The Alethia® Illumigene Malaria kit had a sensitivity of 90.24% (95% CI 76.87–97.28) and a specificity of 95.65% (95% CI 89.24–98.80) as compared to qPCR. Conclusions This head-to-head comparison showed higher performance indicators of the HumaTurb Loopamp™ Malaria PDT kit compared to the Alethia® illumigene Malaria kit for detection of malaria. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 22 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Malaria LAMP Loop-mediated isothermal amplification Diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Malaria LAMP Loop-mediated isothermal amplification Diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Anna-Clara Ivarsson Elin Fransén Ioanna Broumou Anna Färnert Kristina E. M. Persson Sara Karlsson Söbirk Head-to-head comparison of two loop-mediated isothermal amplification (LAMP) kits for diagnosis of malaria in a non-endemic setting |
topic_facet |
Malaria LAMP Loop-mediated isothermal amplification Diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Light microscopy and rapid diagnostic tests (RDT) have long been the recommended diagnostic methods for malaria. However, in recent years, loop-mediated isothermal amplification (LAMP) techniques have been shown to offer superior performance, in particular concerning low-grade parasitaemia, by delivering higher sensitivity and specificity with low laboratory capacity requirements in little more than an hour. In this study, the diagnostic performance of two LAMP kits were assessed head-to-head, compared to highly sensitive quantitative real time PCR (qPCR), in a non-endemic setting. Methods In this retrospective validation study two LAMP kits; Alethia® Illumigene Malaria kit and HumaTurb Loopamp™ Malaria Pan Detection (PDT) kit, were evaluated head-to-head for detection of Plasmodium-DNA in 133 biobanked blood samples from suspected malaria cases at the Clinical Microbiology Laboratory of Region Skåne, Sweden to determine their diagnostic performance compared to qPCR. Results Of the 133 samples tested, qPCR detected Plasmodium DNA in 41 samples (defined as true positives), and the two LAMP methods detected 41 and 37 of those, respectively. The results from the HumaTurb Loopamp™ Malaria PDT kit were in complete congruence with the qPCR, with a sensitivity of 100% (95% CI 91.40–100%) and specificity of 100% (95% CI 96.07–100%). The Alethia® Illumigene Malaria kit had a sensitivity of 90.24% (95% CI 76.87–97.28) and a specificity of 95.65% (95% CI 89.24–98.80) as compared to qPCR. Conclusions This head-to-head comparison showed higher performance indicators of the HumaTurb Loopamp™ Malaria PDT kit compared to the Alethia® illumigene Malaria kit for detection of malaria. |
format |
Article in Journal/Newspaper |
author |
Anna-Clara Ivarsson Elin Fransén Ioanna Broumou Anna Färnert Kristina E. M. Persson Sara Karlsson Söbirk |
author_facet |
Anna-Clara Ivarsson Elin Fransén Ioanna Broumou Anna Färnert Kristina E. M. Persson Sara Karlsson Söbirk |
author_sort |
Anna-Clara Ivarsson |
title |
Head-to-head comparison of two loop-mediated isothermal amplification (LAMP) kits for diagnosis of malaria in a non-endemic setting |
title_short |
Head-to-head comparison of two loop-mediated isothermal amplification (LAMP) kits for diagnosis of malaria in a non-endemic setting |
title_full |
Head-to-head comparison of two loop-mediated isothermal amplification (LAMP) kits for diagnosis of malaria in a non-endemic setting |
title_fullStr |
Head-to-head comparison of two loop-mediated isothermal amplification (LAMP) kits for diagnosis of malaria in a non-endemic setting |
title_full_unstemmed |
Head-to-head comparison of two loop-mediated isothermal amplification (LAMP) kits for diagnosis of malaria in a non-endemic setting |
title_sort |
head-to-head comparison of two loop-mediated isothermal amplification (lamp) kits for diagnosis of malaria in a non-endemic setting |
publisher |
BMC |
publishDate |
2023 |
url |
https://doi.org/10.1186/s12936-023-04809-7 https://doaj.org/article/009af99799d84253ad3af989d0c75b7d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 22, Iss 1, Pp 1-10 (2023) |
op_relation |
https://doi.org/10.1186/s12936-023-04809-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-023-04809-7 1475-2875 https://doaj.org/article/009af99799d84253ad3af989d0c75b7d |
op_doi |
https://doi.org/10.1186/s12936-023-04809-7 |
container_title |
Malaria Journal |
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22 |
container_issue |
1 |
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1788694548320354304 |