In vitro inhibitory effects on α-glucosidase and α-amylase level and antioxidant potential of seeds of Phoenix dactylifera L.
Objective: To evaluate and compare the antioxidant activity, total phenolic contents (TPCs) and in vitro antidiabetic activity of various pits extracts obtained from five Omani date cultivars. Methods: Sun-dried mature fruits of five Omani date varieties, namely, Fardh, Naghal, Khalas, Khinazi and K...
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Wolters Kluwer Medknow Publications
2016
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ftdoajarticles:oai:doaj.org/article:008801ed9da740069cdfa6b7507627a6 2023-05-15T15:12:09+02:00 In vitro inhibitory effects on α-glucosidase and α-amylase level and antioxidant potential of seeds of Phoenix dactylifera L. Shah Alam Khan Amira Rashid Al Kiyumi Manal Saif Al Sheidi Tagreed Salim Al Khusaibi Noura Mohammed Al Shehhi Tanveer Alam 2016-04-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2015.11.008 https://doaj.org/article/008801ed9da740069cdfa6b7507627a6 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169116000095 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.11.008 https://doaj.org/article/008801ed9da740069cdfa6b7507627a6 Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 4, Pp 322-329 (2016) Phoenix dactylifera L Antioxidant In vitro antidiabetic activity Date pits Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2016 ftdoajarticles https://doi.org/10.1016/j.apjtb.2015.11.008 2022-12-31T04:45:32Z Objective: To evaluate and compare the antioxidant activity, total phenolic contents (TPCs) and in vitro antidiabetic activity of various pits extracts obtained from five Omani date cultivars. Methods: Sun-dried mature fruits of five Omani date varieties, namely, Fardh, Naghal, Khalas, Khinazi and Khasab were purchased from the local market in Muscat, Oman in the month of September 2014. Four seed extracts viz. water, ethanol, methanol and acetone were prepared for each date variety and their antioxidant activities were investigated by 1,1-diphenyl-2-picrylhydrazyl, hydrogen peroxide scavenging method and reducing power assay method, respectively. In vitro antidiabetic activity of the date pit extracts was evaluated by measuring their inhibitory effect on α-glucosidase and α-amylase level. TPCs were also quantified colorimetrically. Results: The results indicated that TPC of date seeds was solvent dependent. Acetone, ethanol and methanol were found to be significantly better solvents than water in extracting phenolic compounds from the date seeds. Pit extracts exhibited moderate to good in vitro antioxidant activity and increased reducing power. Among all date pit extracts, water extract exhibited significant in vitro antidiabetic activity in comparison to standard drug, acarbose. Conclusions: The present study confirms that disposed waste of Omani dates is a rich source of dietary antioxidant because of its high TPC. The pits due to their inhibitory effects on α-glucosidase and α-amylase level could be used as a monotherapy along with an appropriate diabetic diet and exercise or might be in conjunction with antidiabetic therapy to manage and prevent progression of diabetes. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 6 4 322 329 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Phoenix dactylifera L Antioxidant In vitro antidiabetic activity Date pits Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Phoenix dactylifera L Antioxidant In vitro antidiabetic activity Date pits Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Shah Alam Khan Amira Rashid Al Kiyumi Manal Saif Al Sheidi Tagreed Salim Al Khusaibi Noura Mohammed Al Shehhi Tanveer Alam In vitro inhibitory effects on α-glucosidase and α-amylase level and antioxidant potential of seeds of Phoenix dactylifera L. |
topic_facet |
Phoenix dactylifera L Antioxidant In vitro antidiabetic activity Date pits Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate and compare the antioxidant activity, total phenolic contents (TPCs) and in vitro antidiabetic activity of various pits extracts obtained from five Omani date cultivars. Methods: Sun-dried mature fruits of five Omani date varieties, namely, Fardh, Naghal, Khalas, Khinazi and Khasab were purchased from the local market in Muscat, Oman in the month of September 2014. Four seed extracts viz. water, ethanol, methanol and acetone were prepared for each date variety and their antioxidant activities were investigated by 1,1-diphenyl-2-picrylhydrazyl, hydrogen peroxide scavenging method and reducing power assay method, respectively. In vitro antidiabetic activity of the date pit extracts was evaluated by measuring their inhibitory effect on α-glucosidase and α-amylase level. TPCs were also quantified colorimetrically. Results: The results indicated that TPC of date seeds was solvent dependent. Acetone, ethanol and methanol were found to be significantly better solvents than water in extracting phenolic compounds from the date seeds. Pit extracts exhibited moderate to good in vitro antioxidant activity and increased reducing power. Among all date pit extracts, water extract exhibited significant in vitro antidiabetic activity in comparison to standard drug, acarbose. Conclusions: The present study confirms that disposed waste of Omani dates is a rich source of dietary antioxidant because of its high TPC. The pits due to their inhibitory effects on α-glucosidase and α-amylase level could be used as a monotherapy along with an appropriate diabetic diet and exercise or might be in conjunction with antidiabetic therapy to manage and prevent progression of diabetes. |
format |
Article in Journal/Newspaper |
author |
Shah Alam Khan Amira Rashid Al Kiyumi Manal Saif Al Sheidi Tagreed Salim Al Khusaibi Noura Mohammed Al Shehhi Tanveer Alam |
author_facet |
Shah Alam Khan Amira Rashid Al Kiyumi Manal Saif Al Sheidi Tagreed Salim Al Khusaibi Noura Mohammed Al Shehhi Tanveer Alam |
author_sort |
Shah Alam Khan |
title |
In vitro inhibitory effects on α-glucosidase and α-amylase level and antioxidant potential of seeds of Phoenix dactylifera L. |
title_short |
In vitro inhibitory effects on α-glucosidase and α-amylase level and antioxidant potential of seeds of Phoenix dactylifera L. |
title_full |
In vitro inhibitory effects on α-glucosidase and α-amylase level and antioxidant potential of seeds of Phoenix dactylifera L. |
title_fullStr |
In vitro inhibitory effects on α-glucosidase and α-amylase level and antioxidant potential of seeds of Phoenix dactylifera L. |
title_full_unstemmed |
In vitro inhibitory effects on α-glucosidase and α-amylase level and antioxidant potential of seeds of Phoenix dactylifera L. |
title_sort |
in vitro inhibitory effects on α-glucosidase and α-amylase level and antioxidant potential of seeds of phoenix dactylifera l. |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.apjtb.2015.11.008 https://doaj.org/article/008801ed9da740069cdfa6b7507627a6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 4, Pp 322-329 (2016) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169116000095 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.11.008 https://doaj.org/article/008801ed9da740069cdfa6b7507627a6 |
op_doi |
https://doi.org/10.1016/j.apjtb.2015.11.008 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
6 |
container_issue |
4 |
container_start_page |
322 |
op_container_end_page |
329 |
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1766342868761313280 |