Post-treatment vascular leakage and inflammatory responses around brain cysts in porcine neurocysticercosis.
Cysticidal treatment of neurocysticercosis, an infection of humans and pig brains with Taenia solium, results in an early inflammatory response directed to cysts causing seizures and focal neurological manifestations. Treatment-induced pericystic inflammation and its association with blood brain bar...
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ftdoajarticles:oai:doaj.org/article:002eb8e711314e688a995a9e105a3b58 2023-05-15T15:16:32+02:00 Post-treatment vascular leakage and inflammatory responses around brain cysts in porcine neurocysticercosis. Siddhartha Mahanty Miguel Angel Orrego Holger Mayta Miguel Marzal Carla Cangalaya Adriana Paredes Eloy Gonzales-Gustavson Gianfranco Arroyo Armando E Gonzalez Cristina Guerra-Giraldez Hector H García Theodore E Nash Cysticercosis Working Group in Peru 2015-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003577 https://doaj.org/article/002eb8e711314e688a995a9e105a3b58 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0003577 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003577 https://doaj.org/article/002eb8e711314e688a995a9e105a3b58 PLoS Neglected Tropical Diseases, Vol 9, Iss 3, p e0003577 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003577 2022-12-31T11:45:13Z Cysticidal treatment of neurocysticercosis, an infection of humans and pig brains with Taenia solium, results in an early inflammatory response directed to cysts causing seizures and focal neurological manifestations. Treatment-induced pericystic inflammation and its association with blood brain barrier (BBB) dysfunction, as determined by Evans blue (EB) extravasation, was studied in infected untreated and anthelmintic-treated pigs. We compared the magnitude and extent of the pericystic inflammation, presence of EB-stained capsules, the level of damage to the parasite, expression of genes for proinflammatory and regulatory cytokines, chemokines, and tissue remodeling by quantitative PCR assays between treated and untreated infected pigs and between EB-stained (blue) and non stained (clear) cysts. Inflammatory scores were higher in pericystic tissues from EB-stained cysts compared to clear cysts from untreated pigs and also from anthelmintic-treated pigs 48 hr and 120 hr after treatment. The degree of inflammation correlated with the severity of cyst wall damage and both increased significantly at 120 hours. Expression levels of the proinflammatory genes for IL-6, IFN-γ, TNF-α were higher in EB-stained cysts compared to clear cysts and unaffected brain tissues, and were generally highest at 120 hr. Additionally, expression of some markers of immunoregulatory activity (IL-10, IL-2Rα) were decreased in EB-stained capsules. An increase in other markers for regulatory T cells (CTLA4, FoxP3) was found, as well as significant increases in expression of two metalloproteases, MMP1 and MMP2 at 48 hr and 120 hr post-treatment. We conclude that the increase in severity of the inflammation caused by treatment is accompanied by both a proinflammatory and a complex regulatory response, largely limited to pericystic tissues with compromised vascular integrity. Because treatment induced inflammation occurs in porcine NCC similar to that in human cases, this model can be used to investigate mechanisms involved in host damaging ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 9 3 e0003577 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Siddhartha Mahanty Miguel Angel Orrego Holger Mayta Miguel Marzal Carla Cangalaya Adriana Paredes Eloy Gonzales-Gustavson Gianfranco Arroyo Armando E Gonzalez Cristina Guerra-Giraldez Hector H García Theodore E Nash Cysticercosis Working Group in Peru Post-treatment vascular leakage and inflammatory responses around brain cysts in porcine neurocysticercosis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Cysticidal treatment of neurocysticercosis, an infection of humans and pig brains with Taenia solium, results in an early inflammatory response directed to cysts causing seizures and focal neurological manifestations. Treatment-induced pericystic inflammation and its association with blood brain barrier (BBB) dysfunction, as determined by Evans blue (EB) extravasation, was studied in infected untreated and anthelmintic-treated pigs. We compared the magnitude and extent of the pericystic inflammation, presence of EB-stained capsules, the level of damage to the parasite, expression of genes for proinflammatory and regulatory cytokines, chemokines, and tissue remodeling by quantitative PCR assays between treated and untreated infected pigs and between EB-stained (blue) and non stained (clear) cysts. Inflammatory scores were higher in pericystic tissues from EB-stained cysts compared to clear cysts from untreated pigs and also from anthelmintic-treated pigs 48 hr and 120 hr after treatment. The degree of inflammation correlated with the severity of cyst wall damage and both increased significantly at 120 hours. Expression levels of the proinflammatory genes for IL-6, IFN-γ, TNF-α were higher in EB-stained cysts compared to clear cysts and unaffected brain tissues, and were generally highest at 120 hr. Additionally, expression of some markers of immunoregulatory activity (IL-10, IL-2Rα) were decreased in EB-stained capsules. An increase in other markers for regulatory T cells (CTLA4, FoxP3) was found, as well as significant increases in expression of two metalloproteases, MMP1 and MMP2 at 48 hr and 120 hr post-treatment. We conclude that the increase in severity of the inflammation caused by treatment is accompanied by both a proinflammatory and a complex regulatory response, largely limited to pericystic tissues with compromised vascular integrity. Because treatment induced inflammation occurs in porcine NCC similar to that in human cases, this model can be used to investigate mechanisms involved in host damaging ... |
format |
Article in Journal/Newspaper |
author |
Siddhartha Mahanty Miguel Angel Orrego Holger Mayta Miguel Marzal Carla Cangalaya Adriana Paredes Eloy Gonzales-Gustavson Gianfranco Arroyo Armando E Gonzalez Cristina Guerra-Giraldez Hector H García Theodore E Nash Cysticercosis Working Group in Peru |
author_facet |
Siddhartha Mahanty Miguel Angel Orrego Holger Mayta Miguel Marzal Carla Cangalaya Adriana Paredes Eloy Gonzales-Gustavson Gianfranco Arroyo Armando E Gonzalez Cristina Guerra-Giraldez Hector H García Theodore E Nash Cysticercosis Working Group in Peru |
author_sort |
Siddhartha Mahanty |
title |
Post-treatment vascular leakage and inflammatory responses around brain cysts in porcine neurocysticercosis. |
title_short |
Post-treatment vascular leakage and inflammatory responses around brain cysts in porcine neurocysticercosis. |
title_full |
Post-treatment vascular leakage and inflammatory responses around brain cysts in porcine neurocysticercosis. |
title_fullStr |
Post-treatment vascular leakage and inflammatory responses around brain cysts in porcine neurocysticercosis. |
title_full_unstemmed |
Post-treatment vascular leakage and inflammatory responses around brain cysts in porcine neurocysticercosis. |
title_sort |
post-treatment vascular leakage and inflammatory responses around brain cysts in porcine neurocysticercosis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0003577 https://doaj.org/article/002eb8e711314e688a995a9e105a3b58 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 3, p e0003577 (2015) |
op_relation |
https://doi.org/10.1371/journal.pntd.0003577 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003577 https://doaj.org/article/002eb8e711314e688a995a9e105a3b58 |
op_doi |
https://doi.org/10.1371/journal.pntd.0003577 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
9 |
container_issue |
3 |
container_start_page |
e0003577 |
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1766346835757105152 |