Study of mutations in IDH1 and IDH2 genes in a sample of gliomas from Colombian population
Introduction: Gliomas are the most common primary tumors of the central nervous system and, according to their malignancy, they are graded from I to IV. Recent studies have found that there is an association between gliomas and mutations in exon 4 of genes that codify for isocitrate dehydrogenases 1...
Published in: | Biomédica |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English Spanish |
Published: |
Instituto Nacional de Salud
2018
|
Subjects: | |
Online Access: | https://doi.org/10.7705/biomedica.v38i0.3708 https://doaj.org/article/0007757794d842b49ab9c6b84ae9e4cf |
id |
ftdoajarticles:oai:doaj.org/article:0007757794d842b49ab9c6b84ae9e4cf |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:0007757794d842b49ab9c6b84ae9e4cf 2023-05-15T15:07:00+02:00 Study of mutations in IDH1 and IDH2 genes in a sample of gliomas from Colombian population Orlando Ricaurte Karina Neita Danyela Valero Jenny Ortega-Rojas Carlos E. Arboleda-Bustos Camilo Zubieta José Penagos Gonzalo Arboleda 2018-05-01T00:00:00Z https://doi.org/10.7705/biomedica.v38i0.3708 https://doaj.org/article/0007757794d842b49ab9c6b84ae9e4cf EN ES eng spa Instituto Nacional de Salud https://www.revistabiomedica.org/index.php/biomedica/article/view/3708 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v38i0.3708 https://doaj.org/article/0007757794d842b49ab9c6b84ae9e4cf Biomédica: revista del Instituto Nacional de Salud, Vol 38, Iss 0, Pp 86-92 (2018) sistema nervioso central glioma ADN de neoplasias Colombia Medicine R Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.7705/biomedica.v38i0.3708 2022-12-31T00:56:26Z Introduction: Gliomas are the most common primary tumors of the central nervous system and, according to their malignancy, they are graded from I to IV. Recent studies have found that there is an association between gliomas and mutations in exon 4 of genes that codify for isocitrate dehydrogenases 1 and 2 (IDH1: codon 132; IDH2: codon 172). Objective: To establish the frequency of mutations in IDH1 and IDH2 in a sample of gliomas from Colombian population. Materials and methods: DNA was extracted from tumor tissue. The exon 4 of IDH1 and IDH2 was amplified by PCR using specific primers and subsequently sequenced. Mutations were determined using the 4Peaks MAFFT programs. Results: We found mutations in the IDH1 gene in 34% of the glioma samples, with a predominance of the nonsynonymous mutation R132H. Mutations in the IDH2 gene were found in 7.5% of cases, with a predominance of the nonsynonymous R172K and R172W mutations. Conclusions: The frequency of mutations in the IDH1 and IDH2 genes in the sample was similar to that reported in other studies. The analysis of these mutations may be important to establish prognostic factors and for the development of future therapeutic targets in gliomas. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Biomédica 38 93 99 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Spanish |
topic |
sistema nervioso central glioma ADN de neoplasias Colombia Medicine R Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
sistema nervioso central glioma ADN de neoplasias Colombia Medicine R Arctic medicine. Tropical medicine RC955-962 Orlando Ricaurte Karina Neita Danyela Valero Jenny Ortega-Rojas Carlos E. Arboleda-Bustos Camilo Zubieta José Penagos Gonzalo Arboleda Study of mutations in IDH1 and IDH2 genes in a sample of gliomas from Colombian population |
topic_facet |
sistema nervioso central glioma ADN de neoplasias Colombia Medicine R Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction: Gliomas are the most common primary tumors of the central nervous system and, according to their malignancy, they are graded from I to IV. Recent studies have found that there is an association between gliomas and mutations in exon 4 of genes that codify for isocitrate dehydrogenases 1 and 2 (IDH1: codon 132; IDH2: codon 172). Objective: To establish the frequency of mutations in IDH1 and IDH2 in a sample of gliomas from Colombian population. Materials and methods: DNA was extracted from tumor tissue. The exon 4 of IDH1 and IDH2 was amplified by PCR using specific primers and subsequently sequenced. Mutations were determined using the 4Peaks MAFFT programs. Results: We found mutations in the IDH1 gene in 34% of the glioma samples, with a predominance of the nonsynonymous mutation R132H. Mutations in the IDH2 gene were found in 7.5% of cases, with a predominance of the nonsynonymous R172K and R172W mutations. Conclusions: The frequency of mutations in the IDH1 and IDH2 genes in the sample was similar to that reported in other studies. The analysis of these mutations may be important to establish prognostic factors and for the development of future therapeutic targets in gliomas. |
format |
Article in Journal/Newspaper |
author |
Orlando Ricaurte Karina Neita Danyela Valero Jenny Ortega-Rojas Carlos E. Arboleda-Bustos Camilo Zubieta José Penagos Gonzalo Arboleda |
author_facet |
Orlando Ricaurte Karina Neita Danyela Valero Jenny Ortega-Rojas Carlos E. Arboleda-Bustos Camilo Zubieta José Penagos Gonzalo Arboleda |
author_sort |
Orlando Ricaurte |
title |
Study of mutations in IDH1 and IDH2 genes in a sample of gliomas from Colombian population |
title_short |
Study of mutations in IDH1 and IDH2 genes in a sample of gliomas from Colombian population |
title_full |
Study of mutations in IDH1 and IDH2 genes in a sample of gliomas from Colombian population |
title_fullStr |
Study of mutations in IDH1 and IDH2 genes in a sample of gliomas from Colombian population |
title_full_unstemmed |
Study of mutations in IDH1 and IDH2 genes in a sample of gliomas from Colombian population |
title_sort |
study of mutations in idh1 and idh2 genes in a sample of gliomas from colombian population |
publisher |
Instituto Nacional de Salud |
publishDate |
2018 |
url |
https://doi.org/10.7705/biomedica.v38i0.3708 https://doaj.org/article/0007757794d842b49ab9c6b84ae9e4cf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Biomédica: revista del Instituto Nacional de Salud, Vol 38, Iss 0, Pp 86-92 (2018) |
op_relation |
https://www.revistabiomedica.org/index.php/biomedica/article/view/3708 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v38i0.3708 https://doaj.org/article/0007757794d842b49ab9c6b84ae9e4cf |
op_doi |
https://doi.org/10.7705/biomedica.v38i0.3708 |
container_title |
Biomédica |
container_volume |
38 |
container_start_page |
93 |
op_container_end_page |
99 |
_version_ |
1766338581386756096 |