Damage Escape and Repair in Dried Chroococcidiopsis spp. from Hot and Cold Deserts Exposed to Simulated Space and Martian Conditions
The cyanobacterium Chroococcidiopsis, overlain by 3mm of Antarctic sandstone, was exposed as dried multilayers to simulated space and martian conditions. Ground-based experiments were conducted in the context of Lichens and Fungi Experiments (EXPOSE-E mission, European Space Agency), which were perf...
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Mary Ann Liebert Inc.
2011
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ftdlr:oai:elib.dlr.de:70836 2023-05-15T13:59:58+02:00 Damage Escape and Repair in Dried Chroococcidiopsis spp. from Hot and Cold Deserts Exposed to Simulated Space and Martian Conditions Billi, Daniela Viaggiu, Emanuela Cockell, Charles S. Rabbow, Elke Horneck, Gerda Onofri, Silvano 2011 https://elib.dlr.de/70836/ unknown Mary Ann Liebert Inc. Billi, Daniela und Viaggiu, Emanuela und Cockell, Charles S. und Rabbow, Elke und Horneck, Gerda und Onofri, Silvano (2011) Damage Escape and Repair in Dried Chroococcidiopsis spp. from Hot and Cold Deserts Exposed to Simulated Space and Martian Conditions. Astrobiology, 11 (1), Seiten 65-73. Mary Ann Liebert Inc. DOI:10.1089/ast.2009.0430 <https://doi.org/10.1089/ast.2009.0430> Strahlenbiologie Zeitschriftenbeitrag PeerReviewed 2011 ftdlr https://doi.org/10.1089/ast.2009.0430 2019-09-08T22:54:08Z The cyanobacterium Chroococcidiopsis, overlain by 3mm of Antarctic sandstone, was exposed as dried multilayers to simulated space and martian conditions. Ground-based experiments were conducted in the context of Lichens and Fungi Experiments (EXPOSE-E mission, European Space Agency), which were performed to evaluate, after 1.5 years on the International Space Station, the survival of cyanobacteria (Chroococcidiopsis), lichens, and fungi colonized on Antarctic rock. The survival potential and the role played by protection and repair mechanisms in the response of dried Chroococcidiopsis cells to ground-based experiments were both investigated. Different methods were employed, including evaluation of the colony-forming ability, single-cell analysis of subcellular integrities based on membrane integrity molecular and redox probes, evaluation of the photosynthetic pigment autofluorescence, and assessment of the genomic DNA integrity with a PCR-based assay. Desiccation survivors of strain CCMEE 123 (coastal desert, Chile) were better suited than CCMEE 134 (Beacon Valley, Antarctica) to withstand cellular damage imposed by simulated space and martian conditions. Exposed dried cells of strain CCMEE 123 formed colonies, maintained subcellular integrities, and, depending on the exposure conditions, also escaped DNA damage or repaired the induced damage upon rewetting. Other Non-Article Part of Journal/Newspaper Antarc* Antarctic Antarctica German Aerospace Center: elib - DLR electronic library Antarctic Beacon Valley ENVELOPE(160.650,160.650,-77.817,-77.817) Astrobiology 11 1 65 73 |
institution |
Open Polar |
collection |
German Aerospace Center: elib - DLR electronic library |
op_collection_id |
ftdlr |
language |
unknown |
topic |
Strahlenbiologie |
spellingShingle |
Strahlenbiologie Billi, Daniela Viaggiu, Emanuela Cockell, Charles S. Rabbow, Elke Horneck, Gerda Onofri, Silvano Damage Escape and Repair in Dried Chroococcidiopsis spp. from Hot and Cold Deserts Exposed to Simulated Space and Martian Conditions |
topic_facet |
Strahlenbiologie |
description |
The cyanobacterium Chroococcidiopsis, overlain by 3mm of Antarctic sandstone, was exposed as dried multilayers to simulated space and martian conditions. Ground-based experiments were conducted in the context of Lichens and Fungi Experiments (EXPOSE-E mission, European Space Agency), which were performed to evaluate, after 1.5 years on the International Space Station, the survival of cyanobacteria (Chroococcidiopsis), lichens, and fungi colonized on Antarctic rock. The survival potential and the role played by protection and repair mechanisms in the response of dried Chroococcidiopsis cells to ground-based experiments were both investigated. Different methods were employed, including evaluation of the colony-forming ability, single-cell analysis of subcellular integrities based on membrane integrity molecular and redox probes, evaluation of the photosynthetic pigment autofluorescence, and assessment of the genomic DNA integrity with a PCR-based assay. Desiccation survivors of strain CCMEE 123 (coastal desert, Chile) were better suited than CCMEE 134 (Beacon Valley, Antarctica) to withstand cellular damage imposed by simulated space and martian conditions. Exposed dried cells of strain CCMEE 123 formed colonies, maintained subcellular integrities, and, depending on the exposure conditions, also escaped DNA damage or repaired the induced damage upon rewetting. |
format |
Other Non-Article Part of Journal/Newspaper |
author |
Billi, Daniela Viaggiu, Emanuela Cockell, Charles S. Rabbow, Elke Horneck, Gerda Onofri, Silvano |
author_facet |
Billi, Daniela Viaggiu, Emanuela Cockell, Charles S. Rabbow, Elke Horneck, Gerda Onofri, Silvano |
author_sort |
Billi, Daniela |
title |
Damage Escape and Repair in Dried Chroococcidiopsis spp. from Hot and Cold Deserts Exposed to Simulated Space and Martian Conditions |
title_short |
Damage Escape and Repair in Dried Chroococcidiopsis spp. from Hot and Cold Deserts Exposed to Simulated Space and Martian Conditions |
title_full |
Damage Escape and Repair in Dried Chroococcidiopsis spp. from Hot and Cold Deserts Exposed to Simulated Space and Martian Conditions |
title_fullStr |
Damage Escape and Repair in Dried Chroococcidiopsis spp. from Hot and Cold Deserts Exposed to Simulated Space and Martian Conditions |
title_full_unstemmed |
Damage Escape and Repair in Dried Chroococcidiopsis spp. from Hot and Cold Deserts Exposed to Simulated Space and Martian Conditions |
title_sort |
damage escape and repair in dried chroococcidiopsis spp. from hot and cold deserts exposed to simulated space and martian conditions |
publisher |
Mary Ann Liebert Inc. |
publishDate |
2011 |
url |
https://elib.dlr.de/70836/ |
long_lat |
ENVELOPE(160.650,160.650,-77.817,-77.817) |
geographic |
Antarctic Beacon Valley |
geographic_facet |
Antarctic Beacon Valley |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
Billi, Daniela und Viaggiu, Emanuela und Cockell, Charles S. und Rabbow, Elke und Horneck, Gerda und Onofri, Silvano (2011) Damage Escape and Repair in Dried Chroococcidiopsis spp. from Hot and Cold Deserts Exposed to Simulated Space and Martian Conditions. Astrobiology, 11 (1), Seiten 65-73. Mary Ann Liebert Inc. DOI:10.1089/ast.2009.0430 <https://doi.org/10.1089/ast.2009.0430> |
op_doi |
https://doi.org/10.1089/ast.2009.0430 |
container_title |
Astrobiology |
container_volume |
11 |
container_issue |
1 |
container_start_page |
65 |
op_container_end_page |
73 |
_version_ |
1766268889464832000 |