Survival, DNA Integrity, and Ultrastructural Damage in Antarctic Cryptoendolithic Eukaryotic Microorganisms Exposed to Ionizing Radiation
Life dispersal between planets, planetary protection, and the search for biosignatures are main topics in astrobiology. Under the umbrella of the STARLIFE project, three Antarctic endolithic microorganisms, the melanized fungus Cryomyces antarcticus CCFEE 515, a hyaline strain of Umbilicaria sp. (CC...
Published in: | Astrobiology |
---|---|
Main Authors: | , , , , , , |
Format: | Other Non-Article Part of Journal/Newspaper |
Language: | unknown |
Published: |
Mary Ann Liebert Inc.
2017
|
Subjects: | |
Online Access: | https://elib.dlr.de/111358/ http://online.liebertpub.com/doi/abs/10.1089/ast.2015.1456 |
id |
ftdlr:oai:elib.dlr.de:111358 |
---|---|
record_format |
openpolar |
spelling |
ftdlr:oai:elib.dlr.de:111358 2023-05-15T13:59:58+02:00 Survival, DNA Integrity, and Ultrastructural Damage in Antarctic Cryptoendolithic Eukaryotic Microorganisms Exposed to Ionizing Radiation Pacelli, Claudia Selbmann, Laura Zucconi, Laura Raguse, Marina Moeller, Ralf Shuryak, Igor Onofri, Silvano 2017 https://elib.dlr.de/111358/ http://online.liebertpub.com/doi/abs/10.1089/ast.2015.1456 unknown Mary Ann Liebert Inc. Pacelli, Claudia und Selbmann, Laura und Zucconi, Laura und Raguse, Marina und Moeller, Ralf und Shuryak, Igor und Onofri, Silvano (2017) Survival, DNA Integrity, and Ultrastructural Damage in Antarctic Cryptoendolithic Eukaryotic Microorganisms Exposed to Ionizing Radiation. Astrobiology, 17 (2), Seiten 126-135. Mary Ann Liebert Inc. DOI:10.1089/ast.2015.1456 <https://doi.org/10.1089/ast.2015.1456> ISSN 1531-1074 Strahlenbiologie Zeitschriftenbeitrag PeerReviewed 2017 ftdlr https://doi.org/10.1089/ast.2015.1456 2019-09-08T22:54:50Z Life dispersal between planets, planetary protection, and the search for biosignatures are main topics in astrobiology. Under the umbrella of the STARLIFE project, three Antarctic endolithic microorganisms, the melanized fungus Cryomyces antarcticus CCFEE 515, a hyaline strain of Umbilicaria sp. (CCFEE 6113, lichenized fungus), and a Stichococcus sp. strain (C45A, green alga), were exposed to high doses of space-relevant gamma radiation (60Co), up to 117.07 kGy. After irradiation survival, DNA integrity and ultrastructural damage were tested. The first was assessed by clonogenic test; viability and dose responses were reasonably described by the linear-quadratic formalism. DNA integrity was evaluated by PCR, and ultrastructural damage was observed by transmission electron microscopy. The most resistant among the tested organisms was C. antarcticus both in terms of colony formation and DNA preservation. Besides, results clearly demonstrate that DNA was well detectable in all the tested organisms even when microorganisms were dead. This high resistance provides support for the use of DNA as a possible biosignature during the next exploration campaigns. Implication in planetary protection and contamination during long-term space travel are put forward. Other Non-Article Part of Journal/Newspaper Antarc* Antarctic antarcticus German Aerospace Center: elib - DLR electronic library Antarctic Astrobiology 17 2 126 135 |
institution |
Open Polar |
collection |
German Aerospace Center: elib - DLR electronic library |
op_collection_id |
ftdlr |
language |
unknown |
topic |
Strahlenbiologie |
spellingShingle |
Strahlenbiologie Pacelli, Claudia Selbmann, Laura Zucconi, Laura Raguse, Marina Moeller, Ralf Shuryak, Igor Onofri, Silvano Survival, DNA Integrity, and Ultrastructural Damage in Antarctic Cryptoendolithic Eukaryotic Microorganisms Exposed to Ionizing Radiation |
topic_facet |
Strahlenbiologie |
description |
Life dispersal between planets, planetary protection, and the search for biosignatures are main topics in astrobiology. Under the umbrella of the STARLIFE project, three Antarctic endolithic microorganisms, the melanized fungus Cryomyces antarcticus CCFEE 515, a hyaline strain of Umbilicaria sp. (CCFEE 6113, lichenized fungus), and a Stichococcus sp. strain (C45A, green alga), were exposed to high doses of space-relevant gamma radiation (60Co), up to 117.07 kGy. After irradiation survival, DNA integrity and ultrastructural damage were tested. The first was assessed by clonogenic test; viability and dose responses were reasonably described by the linear-quadratic formalism. DNA integrity was evaluated by PCR, and ultrastructural damage was observed by transmission electron microscopy. The most resistant among the tested organisms was C. antarcticus both in terms of colony formation and DNA preservation. Besides, results clearly demonstrate that DNA was well detectable in all the tested organisms even when microorganisms were dead. This high resistance provides support for the use of DNA as a possible biosignature during the next exploration campaigns. Implication in planetary protection and contamination during long-term space travel are put forward. |
format |
Other Non-Article Part of Journal/Newspaper |
author |
Pacelli, Claudia Selbmann, Laura Zucconi, Laura Raguse, Marina Moeller, Ralf Shuryak, Igor Onofri, Silvano |
author_facet |
Pacelli, Claudia Selbmann, Laura Zucconi, Laura Raguse, Marina Moeller, Ralf Shuryak, Igor Onofri, Silvano |
author_sort |
Pacelli, Claudia |
title |
Survival, DNA Integrity, and Ultrastructural Damage in Antarctic Cryptoendolithic Eukaryotic Microorganisms Exposed to Ionizing Radiation |
title_short |
Survival, DNA Integrity, and Ultrastructural Damage in Antarctic Cryptoendolithic Eukaryotic Microorganisms Exposed to Ionizing Radiation |
title_full |
Survival, DNA Integrity, and Ultrastructural Damage in Antarctic Cryptoendolithic Eukaryotic Microorganisms Exposed to Ionizing Radiation |
title_fullStr |
Survival, DNA Integrity, and Ultrastructural Damage in Antarctic Cryptoendolithic Eukaryotic Microorganisms Exposed to Ionizing Radiation |
title_full_unstemmed |
Survival, DNA Integrity, and Ultrastructural Damage in Antarctic Cryptoendolithic Eukaryotic Microorganisms Exposed to Ionizing Radiation |
title_sort |
survival, dna integrity, and ultrastructural damage in antarctic cryptoendolithic eukaryotic microorganisms exposed to ionizing radiation |
publisher |
Mary Ann Liebert Inc. |
publishDate |
2017 |
url |
https://elib.dlr.de/111358/ http://online.liebertpub.com/doi/abs/10.1089/ast.2015.1456 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic antarcticus |
genre_facet |
Antarc* Antarctic antarcticus |
op_relation |
Pacelli, Claudia und Selbmann, Laura und Zucconi, Laura und Raguse, Marina und Moeller, Ralf und Shuryak, Igor und Onofri, Silvano (2017) Survival, DNA Integrity, and Ultrastructural Damage in Antarctic Cryptoendolithic Eukaryotic Microorganisms Exposed to Ionizing Radiation. Astrobiology, 17 (2), Seiten 126-135. Mary Ann Liebert Inc. DOI:10.1089/ast.2015.1456 <https://doi.org/10.1089/ast.2015.1456> ISSN 1531-1074 |
op_doi |
https://doi.org/10.1089/ast.2015.1456 |
container_title |
Astrobiology |
container_volume |
17 |
container_issue |
2 |
container_start_page |
126 |
op_container_end_page |
135 |
_version_ |
1766268891263139840 |