Survival of Antarctic Cryptoendolithic Fungi in Simulated Martian Conditions On Board the International Space Station
Dehydrated Antarctic cryptoendolithic communities and colonies of the rock inhabitant black fungi Cryomyces antarcticus (CCFEE 515) and Cryomyces minteri (CCFEE 5187) were exposed as part of the Lichens and Fungi Experiment (LIFE) for 18 months in the European Space Agency's EXPOSE-E facility t...
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Mary Ann Liebert Inc.
2015
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ftdlr:oai:elib.dlr.de:102184 2023-05-15T13:59:58+02:00 Survival of Antarctic Cryptoendolithic Fungi in Simulated Martian Conditions On Board the International Space Station Onofri, Silvano de Vera, Jean Pierre Paul Zucconi, Laura Selbmann, Laura Scalzi, Giuliano Venkateswaran, Kasthuri J. Rabbow, Elke de la Torre, Rosa Horneck, Gerda 2015 https://elib.dlr.de/102184/ https://doi.org/10.1089/ast.2015.1324 unknown Mary Ann Liebert Inc. Onofri, Silvano und de Vera, Jean Pierre Paul und Zucconi, Laura und Selbmann, Laura und Scalzi, Giuliano und Venkateswaran, Kasthuri J. und Rabbow, Elke und de la Torre, Rosa und Horneck, Gerda (2015) Survival of Antarctic Cryptoendolithic Fungi in Simulated Martian Conditions On Board the International Space Station. Astrobiology, 15 (12), Seiten 1052-1059. Mary Ann Liebert Inc. DOI:10.1089/ast.2015.1324 <https://doi.org/10.1089/ast.2015.1324> ISSN 1531-1074 Leitungsbereich PF Strahlenbiologie Zeitschriftenbeitrag PeerReviewed 2015 ftdlr https://doi.org/10.1089/ast.2015.1324 2019-09-08T22:54:41Z Dehydrated Antarctic cryptoendolithic communities and colonies of the rock inhabitant black fungi Cryomyces antarcticus (CCFEE 515) and Cryomyces minteri (CCFEE 5187) were exposed as part of the Lichens and Fungi Experiment (LIFE) for 18 months in the European Space Agency's EXPOSE-E facility to simulated martian conditions aboard the International Space Station (ISS). Upon sample retrieval, survival was proved by testing colony-forming ability, and viability of cells (as integrity of cell membrane) was determined by the propidium monoazide (PMA) assay coupled with quantitative PCR tests. Although less than 10% of the samples exposed to simulated martian conditions were able to proliferate and form colonies, the PMA assay indicated that more than 60% of the cells and rock communities had remained intact after the “Mars exposure.” Furthermore, a high stability of the DNA in the cells was demonstrated. The results contribute to assessing the stability of resistant microorganisms and biosignatures on the surface of Mars, data that are valuable information for further search-for-life experiments on Mars. Other Non-Article Part of Journal/Newspaper Antarc* Antarctic antarcticus German Aerospace Center: elib - DLR electronic library Antarctic Astrobiology 15 12 1052 1059 |
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German Aerospace Center: elib - DLR electronic library |
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Leitungsbereich PF Strahlenbiologie |
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Leitungsbereich PF Strahlenbiologie Onofri, Silvano de Vera, Jean Pierre Paul Zucconi, Laura Selbmann, Laura Scalzi, Giuliano Venkateswaran, Kasthuri J. Rabbow, Elke de la Torre, Rosa Horneck, Gerda Survival of Antarctic Cryptoendolithic Fungi in Simulated Martian Conditions On Board the International Space Station |
topic_facet |
Leitungsbereich PF Strahlenbiologie |
description |
Dehydrated Antarctic cryptoendolithic communities and colonies of the rock inhabitant black fungi Cryomyces antarcticus (CCFEE 515) and Cryomyces minteri (CCFEE 5187) were exposed as part of the Lichens and Fungi Experiment (LIFE) for 18 months in the European Space Agency's EXPOSE-E facility to simulated martian conditions aboard the International Space Station (ISS). Upon sample retrieval, survival was proved by testing colony-forming ability, and viability of cells (as integrity of cell membrane) was determined by the propidium monoazide (PMA) assay coupled with quantitative PCR tests. Although less than 10% of the samples exposed to simulated martian conditions were able to proliferate and form colonies, the PMA assay indicated that more than 60% of the cells and rock communities had remained intact after the “Mars exposure.” Furthermore, a high stability of the DNA in the cells was demonstrated. The results contribute to assessing the stability of resistant microorganisms and biosignatures on the surface of Mars, data that are valuable information for further search-for-life experiments on Mars. |
format |
Other Non-Article Part of Journal/Newspaper |
author |
Onofri, Silvano de Vera, Jean Pierre Paul Zucconi, Laura Selbmann, Laura Scalzi, Giuliano Venkateswaran, Kasthuri J. Rabbow, Elke de la Torre, Rosa Horneck, Gerda |
author_facet |
Onofri, Silvano de Vera, Jean Pierre Paul Zucconi, Laura Selbmann, Laura Scalzi, Giuliano Venkateswaran, Kasthuri J. Rabbow, Elke de la Torre, Rosa Horneck, Gerda |
author_sort |
Onofri, Silvano |
title |
Survival of Antarctic Cryptoendolithic Fungi in Simulated Martian Conditions On Board the International Space Station |
title_short |
Survival of Antarctic Cryptoendolithic Fungi in Simulated Martian Conditions On Board the International Space Station |
title_full |
Survival of Antarctic Cryptoendolithic Fungi in Simulated Martian Conditions On Board the International Space Station |
title_fullStr |
Survival of Antarctic Cryptoendolithic Fungi in Simulated Martian Conditions On Board the International Space Station |
title_full_unstemmed |
Survival of Antarctic Cryptoendolithic Fungi in Simulated Martian Conditions On Board the International Space Station |
title_sort |
survival of antarctic cryptoendolithic fungi in simulated martian conditions on board the international space station |
publisher |
Mary Ann Liebert Inc. |
publishDate |
2015 |
url |
https://elib.dlr.de/102184/ https://doi.org/10.1089/ast.2015.1324 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic antarcticus |
genre_facet |
Antarc* Antarctic antarcticus |
op_relation |
Onofri, Silvano und de Vera, Jean Pierre Paul und Zucconi, Laura und Selbmann, Laura und Scalzi, Giuliano und Venkateswaran, Kasthuri J. und Rabbow, Elke und de la Torre, Rosa und Horneck, Gerda (2015) Survival of Antarctic Cryptoendolithic Fungi in Simulated Martian Conditions On Board the International Space Station. Astrobiology, 15 (12), Seiten 1052-1059. Mary Ann Liebert Inc. DOI:10.1089/ast.2015.1324 <https://doi.org/10.1089/ast.2015.1324> ISSN 1531-1074 |
op_doi |
https://doi.org/10.1089/ast.2015.1324 |
container_title |
Astrobiology |
container_volume |
15 |
container_issue |
12 |
container_start_page |
1052 |
op_container_end_page |
1059 |
_version_ |
1766268890942275584 |