Can scat-based species identification be a misleading sign of presence? More evidences from northeastern Portugal
Species identification of non-invasively collected samples using molecular genetics tools has become an important tool in ecological research. For decades, scat-based ecological studies were almost exclusively rooted in morphological identification of scats, within local context, in the field. Howev...
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Format: | Article in Journal/Newspaper |
Language: | Spanish |
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2020
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Online Access: | https://dialnet.unirioja.es/servlet/oaiart?codigo=7721960 |
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ftdialnet:oai:dialnet.unirioja.es:ART0001612634 |
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openpolar |
institution |
Open Polar |
collection |
Dialnet - Portada de revistas (Universidad de La Rioja) |
op_collection_id |
ftdialnet |
language |
Spanish |
topic |
Mesocarnivore Mitochondrial DNA Monitoring Non-invasive sampling Species molecular identification ADN mitocondrial identificación molecular especies mesocarnívoro monitoreo muestreo no invasivo |
spellingShingle |
Mesocarnivore Mitochondrial DNA Monitoring Non-invasive sampling Species molecular identification ADN mitocondrial identificación molecular especies mesocarnívoro monitoreo muestreo no invasivo Alexandre, Marta Rosalino, Luís Miguel Hipólito, Dário Fonseca, Carlos Ferreira, Eduardo Can scat-based species identification be a misleading sign of presence? More evidences from northeastern Portugal |
topic_facet |
Mesocarnivore Mitochondrial DNA Monitoring Non-invasive sampling Species molecular identification ADN mitocondrial identificación molecular especies mesocarnívoro monitoreo muestreo no invasivo |
description |
Species identification of non-invasively collected samples using molecular genetics tools has become an important tool in ecological research. For decades, scat-based ecological studies were almost exclusively rooted in morphological identification of scats, within local context, in the field. However, this approach raised a controversial debate, due to species and context-specific probability of error and lack of validation. In this study, we aimed to test the accuracy of mesocarnivore scats identification, based on morphological criteria, using a carnivore guild in northeastern Portugal as a model and molecular identification as a standard for accuracy of morphological identifications, within local context. While using only expert-based identifications for comparison with molecular identification standard, we have also compared the identifications performed by observers with different levels of experience. We extracted DNA from 63 scats (NE Portugal), which was successfully amplified/sequenced from 83% (n= 52) of the extracts: 38 were molecularly assigned to red fox (Vulpes vulpes), eight to stone marten (Martes foina), two to pine marten (Martes martes) and domestic dog (Canis lupus familiaris) and one to European badger (Meles meles) and common genet (Genetta genetta). There was a tendency for better performance by more experienced researchers, with 67% of scats being correctly assigned, but differences among observers were not significant. Due to the small sample size, only for foxes and stone martens was possible to estimate the error rate in species assignment, based on morphological criteria. False positive rates (% of times a scat was misassigned to a given species) were 4% for fox samples and 62% for stone marten. False negative rates (i.e. the rate at which a scat of a given species was assigned to another species) reached 29% for fox (scats that were initially assigned to stone marten and domestic dog were in fact from fox) and 25% for stone marten (originally misassigned to weasel, Mustela nivalis), ... |
format |
Article in Journal/Newspaper |
author |
Alexandre, Marta Rosalino, Luís Miguel Hipólito, Dário Fonseca, Carlos Ferreira, Eduardo |
author_facet |
Alexandre, Marta Rosalino, Luís Miguel Hipólito, Dário Fonseca, Carlos Ferreira, Eduardo |
author_sort |
Alexandre, Marta |
title |
Can scat-based species identification be a misleading sign of presence? More evidences from northeastern Portugal |
title_short |
Can scat-based species identification be a misleading sign of presence? More evidences from northeastern Portugal |
title_full |
Can scat-based species identification be a misleading sign of presence? More evidences from northeastern Portugal |
title_fullStr |
Can scat-based species identification be a misleading sign of presence? More evidences from northeastern Portugal |
title_full_unstemmed |
Can scat-based species identification be a misleading sign of presence? More evidences from northeastern Portugal |
title_sort |
can scat-based species identification be a misleading sign of presence? more evidences from northeastern portugal |
publishDate |
2020 |
url |
https://dialnet.unirioja.es/servlet/oaiart?codigo=7721960 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Galemys: Boletín informativo de la Sociedad Española para la conservación y estudio de los mamíferos, ISSN 1137-8700, Vol. 32, Vol. 1, 2020, pags. 41-50 |
op_relation |
https://dialnet.unirioja.es/servlet/oaiart?codigo=7721960 (Revista) ISSN 1137-8700 |
op_rights |
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ftdialnet:oai:dialnet.unirioja.es:ART0001612634 2023-08-20T04:05:50+02:00 Can scat-based species identification be a misleading sign of presence? More evidences from northeastern Portugal Alexandre, Marta Rosalino, Luís Miguel Hipólito, Dário Fonseca, Carlos Ferreira, Eduardo 2020 application/pdf https://dialnet.unirioja.es/servlet/oaiart?codigo=7721960 spa spa https://dialnet.unirioja.es/servlet/oaiart?codigo=7721960 (Revista) ISSN 1137-8700 LICENCIA DE USO: Los documentos a texto completo incluidos en Dialnet son de acceso libre y propiedad de sus autores y/o editores. Por tanto, cualquier acto de reproducción, distribución, comunicación pública y/o transformación total o parcial requiere el consentimiento expreso y escrito de aquéllos. Cualquier enlace al texto completo de estos documentos deberá hacerse a través de la URL oficial de éstos en Dialnet. Más información: https://dialnet.unirioja.es/info/derechosOAI | INTELLECTUAL PROPERTY RIGHTS STATEMENT: Full text documents hosted by Dialnet are protected by copyright and/or related rights. This digital object is accessible without charge, but its use is subject to the licensing conditions set by its authors or editors. Unless expressly stated otherwise in the licensing conditions, you are free to linking, browsing, printing and making a copy for your own personal purposes. All other acts of reproduction and communication to the public are subject to the licensing conditions expressed by editors and authors and require consent from them. Any link to this document should be made using its official URL in Dialnet. More info: https://dialnet.unirioja.es/info/derechosOAI Galemys: Boletín informativo de la Sociedad Española para la conservación y estudio de los mamíferos, ISSN 1137-8700, Vol. 32, Vol. 1, 2020, pags. 41-50 Mesocarnivore Mitochondrial DNA Monitoring Non-invasive sampling Species molecular identification ADN mitocondrial identificación molecular especies mesocarnívoro monitoreo muestreo no invasivo text (article) 2020 ftdialnet 2023-07-26T11:34:14Z Species identification of non-invasively collected samples using molecular genetics tools has become an important tool in ecological research. For decades, scat-based ecological studies were almost exclusively rooted in morphological identification of scats, within local context, in the field. However, this approach raised a controversial debate, due to species and context-specific probability of error and lack of validation. In this study, we aimed to test the accuracy of mesocarnivore scats identification, based on morphological criteria, using a carnivore guild in northeastern Portugal as a model and molecular identification as a standard for accuracy of morphological identifications, within local context. While using only expert-based identifications for comparison with molecular identification standard, we have also compared the identifications performed by observers with different levels of experience. We extracted DNA from 63 scats (NE Portugal), which was successfully amplified/sequenced from 83% (n= 52) of the extracts: 38 were molecularly assigned to red fox (Vulpes vulpes), eight to stone marten (Martes foina), two to pine marten (Martes martes) and domestic dog (Canis lupus familiaris) and one to European badger (Meles meles) and common genet (Genetta genetta). There was a tendency for better performance by more experienced researchers, with 67% of scats being correctly assigned, but differences among observers were not significant. Due to the small sample size, only for foxes and stone martens was possible to estimate the error rate in species assignment, based on morphological criteria. False positive rates (% of times a scat was misassigned to a given species) were 4% for fox samples and 62% for stone marten. False negative rates (i.e. the rate at which a scat of a given species was assigned to another species) reached 29% for fox (scats that were initially assigned to stone marten and domestic dog were in fact from fox) and 25% for stone marten (originally misassigned to weasel, Mustela nivalis), ... Article in Journal/Newspaper Canis lupus Dialnet - Portada de revistas (Universidad de La Rioja) |