Are 45 years of reproductive isolation enough to prevent the amplification of mitochondrial genes in the Pacific oyster?
The Pacific oyster culture in Mexico began 45 years ago, first with spat imported from the USA and now with spat produced in several local hatcheries. Oyster farmers do not know the parameters that define the quality of the spat they buy, among them the level of genetic variability available in the...
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Format: | Article in Journal/Newspaper |
Language: | English |
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2019
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Online Access: | https://dialnet.unirioja.es/servlet/oaiart?codigo=8277917 |
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ftdialnet:oai:dialnet.unirioja.es:ART0001528599 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Dialnet - Portada de revistas (Universidad de La Rioja) |
op_collection_id |
ftdialnet |
language |
English |
topic |
Crassostrea gigas Aquaculture Oyster culture mitochondrial DNA Population genetics Acuacultura Ostricultura ADN mitochondrial Genética de poblaciones |
spellingShingle |
Crassostrea gigas Aquaculture Oyster culture mitochondrial DNA Population genetics Acuacultura Ostricultura ADN mitochondrial Genética de poblaciones Reynaga Franco, Felipe de Jesus Grijalva Chon, José Manuel Chávez Villalba, Jorge Eduardo Castro Longoria, Reina Arreola Lizárraga, José Alfredo Barraza Guardado, Ramón Héctor Are 45 years of reproductive isolation enough to prevent the amplification of mitochondrial genes in the Pacific oyster? |
topic_facet |
Crassostrea gigas Aquaculture Oyster culture mitochondrial DNA Population genetics Acuacultura Ostricultura ADN mitochondrial Genética de poblaciones |
description |
The Pacific oyster culture in Mexico began 45 years ago, first with spat imported from the USA and now with spat produced in several local hatcheries. Oyster farmers do not know the parameters that define the quality of the spat they buy, among them the level of genetic variability available in the lots offered. In order to evaluate and compare the genetic variability in spat produced by four Mexican hatcheries, an attempt was made to amplify and sequence the non-coding region and the ND5 gene of the oyster mitochondrial DNA with oligos reported in the scientific literature. The amplification of the non-coding region was not possible due to the bad design of the oligos. Despite the integrity of the extracted oyster DNA, the ND5 gene was not able to be amplified possibly due to the modification of the oligo recognition site in the gene. The generational separation of the oyster cultivated in Mexico from its original source population makes it necessary to obtain new mitochondrial sequences in order to design new oligos suitable for the populations established in Mexico. El cultivo del ostión del Pacífico en México inició hace 45 años, primero con semilla importada de los EUA y ahora con semilla producida en laboratorios locales. Los ostricultores no conocen los parámetros que definen la calidad de la semilla que ellos compran, como el nivel de la variabilidad genética disponible en los lotes ofertados. Con el fin de evaluar y comparar la variabilidad genética de la semilla producida en cuatro laboratorios mexicanos, se hizo un intento de amplificar y secuenciar la región no codificante y el gen ND5 mitocondriales del ostión con oligos reportados en la literatura científica. La amplificación de la región no codificante no fue posible debido al mal diseño de los oligos. A pesar de la integridad del ADN extraído de los ostiones, el gen ND5 no pudo ser amplificado debido posiblemente a la modificación del sitio de reconocimiento del oligo en ese gen. La separación generacional del ostión cultivado en México de su ... |
format |
Article in Journal/Newspaper |
author |
Reynaga Franco, Felipe de Jesus Grijalva Chon, José Manuel Chávez Villalba, Jorge Eduardo Castro Longoria, Reina Arreola Lizárraga, José Alfredo Barraza Guardado, Ramón Héctor |
author_facet |
Reynaga Franco, Felipe de Jesus Grijalva Chon, José Manuel Chávez Villalba, Jorge Eduardo Castro Longoria, Reina Arreola Lizárraga, José Alfredo Barraza Guardado, Ramón Héctor |
author_sort |
Reynaga Franco, Felipe de Jesus |
title |
Are 45 years of reproductive isolation enough to prevent the amplification of mitochondrial genes in the Pacific oyster? |
title_short |
Are 45 years of reproductive isolation enough to prevent the amplification of mitochondrial genes in the Pacific oyster? |
title_full |
Are 45 years of reproductive isolation enough to prevent the amplification of mitochondrial genes in the Pacific oyster? |
title_fullStr |
Are 45 years of reproductive isolation enough to prevent the amplification of mitochondrial genes in the Pacific oyster? |
title_full_unstemmed |
Are 45 years of reproductive isolation enough to prevent the amplification of mitochondrial genes in the Pacific oyster? |
title_sort |
are 45 years of reproductive isolation enough to prevent the amplification of mitochondrial genes in the pacific oyster? |
publishDate |
2019 |
url |
https://dialnet.unirioja.es/servlet/oaiart?codigo=8277917 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
AquaTechnica: Revista Iberoamericana de Acuicultura, ISSN 2737-6095, Vol. 1, Nº. 1, 2019, pags. 22-27 |
op_relation |
https://dialnet.unirioja.es/servlet/oaiart?codigo=8277917 (Revista) ISSN 2737-6095 |
op_rights |
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ftdialnet:oai:dialnet.unirioja.es:ART0001528599 2023-05-15T15:59:09+02:00 Are 45 years of reproductive isolation enough to prevent the amplification of mitochondrial genes in the Pacific oyster? Reynaga Franco, Felipe de Jesus Grijalva Chon, José Manuel Chávez Villalba, Jorge Eduardo Castro Longoria, Reina Arreola Lizárraga, José Alfredo Barraza Guardado, Ramón Héctor 2019 application/pdf https://dialnet.unirioja.es/servlet/oaiart?codigo=8277917 eng eng https://dialnet.unirioja.es/servlet/oaiart?codigo=8277917 (Revista) ISSN 2737-6095 LICENCIA DE USO: Los documentos a texto completo incluidos en Dialnet son de acceso libre y propiedad de sus autores y/o editores. Por tanto, cualquier acto de reproducción, distribución, comunicación pública y/o transformación total o parcial requiere el consentimiento expreso y escrito de aquéllos. Cualquier enlace al texto completo de estos documentos deberá hacerse a través de la URL oficial de éstos en Dialnet. Más información: https://dialnet.unirioja.es/info/derechosOAI | INTELLECTUAL PROPERTY RIGHTS STATEMENT: Full text documents hosted by Dialnet are protected by copyright and/or related rights. This digital object is accessible without charge, but its use is subject to the licensing conditions set by its authors or editors. Unless expressly stated otherwise in the licensing conditions, you are free to linking, browsing, printing and making a copy for your own personal purposes. All other acts of reproduction and communication to the public are subject to the licensing conditions expressed by editors and authors and require consent from them. Any link to this document should be made using its official URL in Dialnet. More info: https://dialnet.unirioja.es/info/derechosOAI AquaTechnica: Revista Iberoamericana de Acuicultura, ISSN 2737-6095, Vol. 1, Nº. 1, 2019, pags. 22-27 Crassostrea gigas Aquaculture Oyster culture mitochondrial DNA Population genetics Acuacultura Ostricultura ADN mitochondrial Genética de poblaciones text (article) 2019 ftdialnet 2022-05-11T07:44:06Z The Pacific oyster culture in Mexico began 45 years ago, first with spat imported from the USA and now with spat produced in several local hatcheries. Oyster farmers do not know the parameters that define the quality of the spat they buy, among them the level of genetic variability available in the lots offered. In order to evaluate and compare the genetic variability in spat produced by four Mexican hatcheries, an attempt was made to amplify and sequence the non-coding region and the ND5 gene of the oyster mitochondrial DNA with oligos reported in the scientific literature. The amplification of the non-coding region was not possible due to the bad design of the oligos. Despite the integrity of the extracted oyster DNA, the ND5 gene was not able to be amplified possibly due to the modification of the oligo recognition site in the gene. The generational separation of the oyster cultivated in Mexico from its original source population makes it necessary to obtain new mitochondrial sequences in order to design new oligos suitable for the populations established in Mexico. El cultivo del ostión del Pacífico en México inició hace 45 años, primero con semilla importada de los EUA y ahora con semilla producida en laboratorios locales. Los ostricultores no conocen los parámetros que definen la calidad de la semilla que ellos compran, como el nivel de la variabilidad genética disponible en los lotes ofertados. Con el fin de evaluar y comparar la variabilidad genética de la semilla producida en cuatro laboratorios mexicanos, se hizo un intento de amplificar y secuenciar la región no codificante y el gen ND5 mitocondriales del ostión con oligos reportados en la literatura científica. La amplificación de la región no codificante no fue posible debido al mal diseño de los oligos. A pesar de la integridad del ADN extraído de los ostiones, el gen ND5 no pudo ser amplificado debido posiblemente a la modificación del sitio de reconocimiento del oligo en ese gen. La separación generacional del ostión cultivado en México de su ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Dialnet - Portada de revistas (Universidad de La Rioja) Pacific |