Identificación de bacterias de la familia Anaplasmataceae en un albergue canino del municipio de Caldas, Antioquia

• Main Authors: Posada Zapata, John, Cabrera, Azucena, González–Alvarez, Dubán, Rodas G, Juan, Monsalve, Santiago, Londoño, Andrés F.
• Format: Article in Journal/Newspaper
• Language: Spanish
• Published: 2017
• Subjects: Canis lupus
• Online Access: https://dialnet.unirioja.es/servlet/oaiart?codigo=8286157

Objetive. It was to detect the circulation of microorganisms of the family Anaplasmataceae in a canine albergo of the municipality of Caldas. Materials and methods. In view of the above, a descriptive study was conducted in 46 dogs (Canis lupus familiaris) to evaluate the presence of Ehrlichia spp. and Anaplasma spp. by molecular techniques or by immunodiagnostic, in a dog shelter of the municipality of Caldas. The DNA extraction was done in whole blood samples with EDTA. Primers were used against dsb and groEL genes to detect Ehrlichia and Anaplasma sequences respectively and phylogenetic analyzes of the sequences obtained were also carried out. On the other hand, SNAP 4DX® was used for serodiagnosis. Results. In the molecular testing, five positive samples were obtained for the Ehrlichia test and three for the Anaplasma test. In the serological tests, three individuals were positive for Ehrlichia spp., three for Anaplasma spp. and for the other trials measured by the SNAP (Dirofilaria immitis y Borrelia burgdorfe) the result was negative. Conclusions. It demonstrated the circulation of Ehrlichia canis and Anaplasma platys in a dog shelter of the municipality of Caldas, Antioquia. Objetivo. Fue detectar la circulación de microorganismos de la familia Anaplasmataceae en un alberge canino del municipio de Caldas. Materiales y métodos. Se realizó un estudio descriptivo en 46 perros (Canis lupus familiaris) para evaluar por técnicas moleculares o por inmunodiagnóstico la presencia de Ehrlichia spp. y Anaplasma spp., en un albergue canino del município de Caldas. Se hizo extracción de ADN en muestras de sangre entera con EDTA y se utilizaron cebadores contra los genes dsb y groEl, para detectar secuencias de Ehrlichia y Anaplasma respectivamente y análisis filogenéticos de las secuencias obtenidas. Por otro lado se usó el SNAP 4DX® para el serodiagnóstico. Resultados. En las pruebas moleculares se obtuvieron cinco muestras positivas para Ehrlichia y tres para Anamaplasma. En las pruebas serológicas, tres ...