Production of an extracellular protease by an Antarctic bacterial isolate (Bacillus sp. JSP1) as a potential feed additive
Extracellular proteolytic activity was found in JSP1, an Antarctic bacterial isolate. The strain was related to Bacillus sp, based on 16S rRNA gene sequence analysis. The JSP1 protease was partially purified by ammonium sulfate precipitation. Optimal enzyme activity occurred at 40 oC and pH 7.4. Enz...
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Universidad de Antioquia: Facultad de Ciencias Agrarias
2011
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Dialnet - Portada de revistas (Universidad de La Rioja) |
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English |
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antarctic Bacillus sp feed additive keratin livestock production protease antártida aditivo alimenticio producción animal proteasa queratina |
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antarctic Bacillus sp feed additive keratin livestock production protease antártida aditivo alimenticio producción animal proteasa queratina Cho, Jaiesoon Park, Inkyung Production of an extracellular protease by an Antarctic bacterial isolate (Bacillus sp. JSP1) as a potential feed additive |
topic_facet |
antarctic Bacillus sp feed additive keratin livestock production protease antártida aditivo alimenticio producción animal proteasa queratina |
description |
Extracellular proteolytic activity was found in JSP1, an Antarctic bacterial isolate. The strain was related to Bacillus sp, based on 16S rRNA gene sequence analysis. The JSP1 protease was partially purified by ammonium sulfate precipitation. Optimal enzyme activity occurred at 40 oC and pH 7.4. Enzyme activity was significantly enhanced in the presence of Mg2+ and Ca2+ and was completely inactivated in presence of Cu2+, Zn2+, Hg2+, EDTA and SDS. The enzyme hydrolyzed casein the most effectively among the protein substrates tested. The enzyme also exhibited relatively high activity on keratin and gluten, and was active against peptidyl conjugates such as L-Leu-p-Nitroanilide and N-Succinyl-L-Phe-p-Nitroanilide. This study suggests that JSP1 protease could be utilized as a potential environmentally-friendly feed additive in animal production. A actividade proteolítica extracelular foi encontrada a partir de uma bactéria antárctica denominada JSP1, mediante uma análises de sequencia do gene 16S rRNA, a cepa foi relacionada para Bacillus sp. A proteasa JSP1 foi parcialmente purificada por precipitação com sulfato de amônia. Uma óptima actividade enzimática ocorreu em 40 oC e pH 7,4. A actividade da enzima foi significativamente maior na presença de Mg2 + e Ca2 +, e foi completamente inactivada em presença de Cu2 + Zn2 +, Hg2 +, EDTA e SDS. A enzima hidrolisada de caseína foi mais eficaz entre os substratos de proteína testados, apresentando maior actividade na queratina e no glúten e foi activo contra os peptidil conjugados como L-Leu-p Nitroanilida e N-succinil-L-Phe-p-Nitroanilida. A enzima pode ser utilizado como aditivo ambiental na alimentação animal. Se reporta haber encontrado actividad proteolítica extracelular en una bacteria antártica denominada JSP1. Con base en el análisis de secuencia genética 16S ARNr, la cepa fué relacionada con Bacillus sp. La proteasa JSP1 fué parcialmente purificada por precipitación con sulfato de amonio. La actividad óptima de la enzima se produjo a 40 ºC y pH 7,4. La ... |
format |
Article in Journal/Newspaper |
author |
Cho, Jaiesoon Park, Inkyung |
author_facet |
Cho, Jaiesoon Park, Inkyung |
author_sort |
Cho, Jaiesoon |
title |
Production of an extracellular protease by an Antarctic bacterial isolate (Bacillus sp. JSP1) as a potential feed additive |
title_short |
Production of an extracellular protease by an Antarctic bacterial isolate (Bacillus sp. JSP1) as a potential feed additive |
title_full |
Production of an extracellular protease by an Antarctic bacterial isolate (Bacillus sp. JSP1) as a potential feed additive |
title_fullStr |
Production of an extracellular protease by an Antarctic bacterial isolate (Bacillus sp. JSP1) as a potential feed additive |
title_full_unstemmed |
Production of an extracellular protease by an Antarctic bacterial isolate (Bacillus sp. JSP1) as a potential feed additive |
title_sort |
production of an extracellular protease by an antarctic bacterial isolate (bacillus sp. jsp1) as a potential feed additive |
publisher |
Universidad de Antioquia: Facultad de Ciencias Agrarias |
publishDate |
2011 |
url |
https://dialnet.unirioja.es/servlet/oaiart?codigo=3637145 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antártica Antártida |
genre_facet |
Antarc* Antarctic Antártica Antártida |
op_source |
Revista Colombiana de Ciencias Pecuarias, ISSN 0120-0690, Vol. 24, Nº. 1, 2011, pags. 3-10 |
op_relation |
https://dialnet.unirioja.es/servlet/oaiart?codigo=3637145 (Revista) ISSN 0120-0690 |
op_rights |
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ftdialnet:oai:dialnet.unirioja.es:ART0000403632 2023-05-15T13:59:51+02:00 Production of an extracellular protease by an Antarctic bacterial isolate (Bacillus sp. JSP1) as a potential feed additive Cho, Jaiesoon Park, Inkyung 2011 application/pdf https://dialnet.unirioja.es/servlet/oaiart?codigo=3637145 eng eng Universidad de Antioquia: Facultad de Ciencias Agrarias https://dialnet.unirioja.es/servlet/oaiart?codigo=3637145 (Revista) ISSN 0120-0690 LICENCIA DE USO: Los documentos a texto completo incluidos en Dialnet son de acceso libre y propiedad de sus autores y/o editores. Por tanto, cualquier acto de reproducción, distribución, comunicación pública y/o transformación total o parcial requiere el consentimiento expreso y escrito de aquéllos. Cualquier enlace al texto completo de estos documentos deberá hacerse a través de la URL oficial de éstos en Dialnet. Más información: https://dialnet.unirioja.es/info/derechosOAI | INTELLECTUAL PROPERTY RIGHTS STATEMENT: Full text documents hosted by Dialnet are protected by copyright and/or related rights. This digital object is accessible without charge, but its use is subject to the licensing conditions set by its authors or editors. Unless expressly stated otherwise in the licensing conditions, you are free to linking, browsing, printing and making a copy for your own personal purposes. All other acts of reproduction and communication to the public are subject to the licensing conditions expressed by editors and authors and require consent from them. Any link to this document should be made using its official URL in Dialnet. More info: https://dialnet.unirioja.es/info/derechosOAI Revista Colombiana de Ciencias Pecuarias, ISSN 0120-0690, Vol. 24, Nº. 1, 2011, pags. 3-10 antarctic Bacillus sp feed additive keratin livestock production protease antártida aditivo alimenticio producción animal proteasa queratina text (article) 2011 ftdialnet 2019-08-29T08:56:36Z Extracellular proteolytic activity was found in JSP1, an Antarctic bacterial isolate. The strain was related to Bacillus sp, based on 16S rRNA gene sequence analysis. The JSP1 protease was partially purified by ammonium sulfate precipitation. Optimal enzyme activity occurred at 40 oC and pH 7.4. Enzyme activity was significantly enhanced in the presence of Mg2+ and Ca2+ and was completely inactivated in presence of Cu2+, Zn2+, Hg2+, EDTA and SDS. The enzyme hydrolyzed casein the most effectively among the protein substrates tested. The enzyme also exhibited relatively high activity on keratin and gluten, and was active against peptidyl conjugates such as L-Leu-p-Nitroanilide and N-Succinyl-L-Phe-p-Nitroanilide. This study suggests that JSP1 protease could be utilized as a potential environmentally-friendly feed additive in animal production. A actividade proteolítica extracelular foi encontrada a partir de uma bactéria antárctica denominada JSP1, mediante uma análises de sequencia do gene 16S rRNA, a cepa foi relacionada para Bacillus sp. A proteasa JSP1 foi parcialmente purificada por precipitação com sulfato de amônia. Uma óptima actividade enzimática ocorreu em 40 oC e pH 7,4. A actividade da enzima foi significativamente maior na presença de Mg2 + e Ca2 +, e foi completamente inactivada em presença de Cu2 + Zn2 +, Hg2 +, EDTA e SDS. A enzima hidrolisada de caseína foi mais eficaz entre os substratos de proteína testados, apresentando maior actividade na queratina e no glúten e foi activo contra os peptidil conjugados como L-Leu-p Nitroanilida e N-succinil-L-Phe-p-Nitroanilida. A enzima pode ser utilizado como aditivo ambiental na alimentação animal. Se reporta haber encontrado actividad proteolítica extracelular en una bacteria antártica denominada JSP1. Con base en el análisis de secuencia genética 16S ARNr, la cepa fué relacionada con Bacillus sp. La proteasa JSP1 fué parcialmente purificada por precipitación con sulfato de amonio. La actividad óptima de la enzima se produjo a 40 ºC y pH 7,4. La ... Article in Journal/Newspaper Antarc* Antarctic Antártica Antártida Dialnet - Portada de revistas (Universidad de La Rioja) Antarctic |