Structural basis of bacterial defense against g-type lysozyme-based innate immunity
Gram-negative bacteria can produce specific proteinaceous inhibitors to defend themselves against the lytic action of host lysozymes. So far, four different lysozyme inhibitor families have been identified. Here, we report the crystal structure of the Escherichia coli periplasmic lysozyme inhibitor...
Published in: | Cellular and Molecular Life Sciences |
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Language: | English |
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Birkhäuser
2013
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ftdesyvdb:oai:bib-pubdb1.desy.de:142547 2023-05-15T15:32:35+02:00 Structural basis of bacterial defense against g-type lysozyme-based innate immunity Leysen, S. Vanderkelen, L. Weeks, S. D. Michiels, C. W. Strelkov, S. V. DE 2013 https://bib-pubdb1.desy.de/record/142547 https://bib-pubdb1.desy.de/search?p=id:%22PHPPUBDB-25506%22 eng eng Birkhäuser info:eu-repo/semantics/altIdentifier/issn/1420-682X info:eu-repo/semantics/altIdentifier/issn/0014-4754 info:eu-repo/semantics/altIdentifier/pmid/pmid:23086131 info:eu-repo/semantics/altIdentifier/issn/1420-9071 info:eu-repo/semantics/altIdentifier/wos/WOS:000315343600011 info:eu-repo/semantics/altIdentifier/doi/10.1007/s00018-012-1184-1 https://bib-pubdb1.desy.de/record/142547 https://bib-pubdb1.desy.de/search?p=id:%22PHPPUBDB-25506%22 info:eu-repo/semantics/closedAccess Cellular and molecular life sciences 70, 1-10 (2013). doi:10.1007/s00018-012-1184-1 info:eu-repo/classification/ddc/570 Animals Crystallography Escherichia coli: chemistry Escherichia coli: immunology Escherichia coli Proteins: chemistry Escherichia coli Proteins: metabolism Immunity Innate: immunology Models Molecular Muramidase: chemistry Muramidase: metabolism Protein Conformation Salmo salar Escherichia coli Proteins PliG protein E coli Muramidase info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2013 ftdesyvdb https://doi.org/10.1007/s00018-012-1184-1 2022-06-30T20:09:37Z Gram-negative bacteria can produce specific proteinaceous inhibitors to defend themselves against the lytic action of host lysozymes. So far, four different lysozyme inhibitor families have been identified. Here, we report the crystal structure of the Escherichia coli periplasmic lysozyme inhibitor of g-type lysozyme (PliG-Ec) in complex with Atlantic salmon g-type lysozyme (SalG) at a resolution of 0.95 Å, which is exceptionally high for a complex of two proteins. The structure reveals for the first time the mechanism of g-type lysozyme inhibition by the PliG family. The latter contains two specific conserved regions that are essential for its inhibitory activity. The inhibitory complex formation is based on a double 'key-lock' mechanism. The first key-lock element is formed by the insertion of two conserved PliG regions into the active site of the lysozyme. The second element is defined by a distinct pocket of PliG accommodating a lysozyme loop. Computational analysis indicates that this pocket represents a suitable site for small molecule binding, which opens an avenue for the development of novel antibacterial agents that suppress the inhibitory activity of PliG. Article in Journal/Newspaper Atlantic salmon Salmo salar DESY Publication Database (PUBDB) Cellular and Molecular Life Sciences 70 6 1113 1122 |
institution |
Open Polar |
collection |
DESY Publication Database (PUBDB) |
op_collection_id |
ftdesyvdb |
language |
English |
topic |
info:eu-repo/classification/ddc/570 Animals Crystallography Escherichia coli: chemistry Escherichia coli: immunology Escherichia coli Proteins: chemistry Escherichia coli Proteins: metabolism Immunity Innate: immunology Models Molecular Muramidase: chemistry Muramidase: metabolism Protein Conformation Salmo salar Escherichia coli Proteins PliG protein E coli Muramidase |
spellingShingle |
info:eu-repo/classification/ddc/570 Animals Crystallography Escherichia coli: chemistry Escherichia coli: immunology Escherichia coli Proteins: chemistry Escherichia coli Proteins: metabolism Immunity Innate: immunology Models Molecular Muramidase: chemistry Muramidase: metabolism Protein Conformation Salmo salar Escherichia coli Proteins PliG protein E coli Muramidase Leysen, S. Vanderkelen, L. Weeks, S. D. Michiels, C. W. Strelkov, S. V. Structural basis of bacterial defense against g-type lysozyme-based innate immunity |
topic_facet |
info:eu-repo/classification/ddc/570 Animals Crystallography Escherichia coli: chemistry Escherichia coli: immunology Escherichia coli Proteins: chemistry Escherichia coli Proteins: metabolism Immunity Innate: immunology Models Molecular Muramidase: chemistry Muramidase: metabolism Protein Conformation Salmo salar Escherichia coli Proteins PliG protein E coli Muramidase |
description |
Gram-negative bacteria can produce specific proteinaceous inhibitors to defend themselves against the lytic action of host lysozymes. So far, four different lysozyme inhibitor families have been identified. Here, we report the crystal structure of the Escherichia coli periplasmic lysozyme inhibitor of g-type lysozyme (PliG-Ec) in complex with Atlantic salmon g-type lysozyme (SalG) at a resolution of 0.95 Å, which is exceptionally high for a complex of two proteins. The structure reveals for the first time the mechanism of g-type lysozyme inhibition by the PliG family. The latter contains two specific conserved regions that are essential for its inhibitory activity. The inhibitory complex formation is based on a double 'key-lock' mechanism. The first key-lock element is formed by the insertion of two conserved PliG regions into the active site of the lysozyme. The second element is defined by a distinct pocket of PliG accommodating a lysozyme loop. Computational analysis indicates that this pocket represents a suitable site for small molecule binding, which opens an avenue for the development of novel antibacterial agents that suppress the inhibitory activity of PliG. |
format |
Article in Journal/Newspaper |
author |
Leysen, S. Vanderkelen, L. Weeks, S. D. Michiels, C. W. Strelkov, S. V. |
author_facet |
Leysen, S. Vanderkelen, L. Weeks, S. D. Michiels, C. W. Strelkov, S. V. |
author_sort |
Leysen, S. |
title |
Structural basis of bacterial defense against g-type lysozyme-based innate immunity |
title_short |
Structural basis of bacterial defense against g-type lysozyme-based innate immunity |
title_full |
Structural basis of bacterial defense against g-type lysozyme-based innate immunity |
title_fullStr |
Structural basis of bacterial defense against g-type lysozyme-based innate immunity |
title_full_unstemmed |
Structural basis of bacterial defense against g-type lysozyme-based innate immunity |
title_sort |
structural basis of bacterial defense against g-type lysozyme-based innate immunity |
publisher |
Birkhäuser |
publishDate |
2013 |
url |
https://bib-pubdb1.desy.de/record/142547 https://bib-pubdb1.desy.de/search?p=id:%22PHPPUBDB-25506%22 |
op_coverage |
DE |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Cellular and molecular life sciences 70, 1-10 (2013). doi:10.1007/s00018-012-1184-1 |
op_relation |
info:eu-repo/semantics/altIdentifier/issn/1420-682X info:eu-repo/semantics/altIdentifier/issn/0014-4754 info:eu-repo/semantics/altIdentifier/pmid/pmid:23086131 info:eu-repo/semantics/altIdentifier/issn/1420-9071 info:eu-repo/semantics/altIdentifier/wos/WOS:000315343600011 info:eu-repo/semantics/altIdentifier/doi/10.1007/s00018-012-1184-1 https://bib-pubdb1.desy.de/record/142547 https://bib-pubdb1.desy.de/search?p=id:%22PHPPUBDB-25506%22 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1007/s00018-012-1184-1 |
container_title |
Cellular and Molecular Life Sciences |
container_volume |
70 |
container_issue |
6 |
container_start_page |
1113 |
op_container_end_page |
1122 |
_version_ |
1766363070159912960 |