Structural characterization of recombinant crustacyanin subunits from the lobster Homarus americanus
Crustacean crustacyanin proteins are linked to the production and modification of carapace colour, with direct implications for fitness and survival. Here, the structural and functional properties of the two recombinant crustacyanin subunits H(1) and H(2) from the American lobster Homarus americanus...
Published in: | Acta Crystallographica Section F Structural Biology and Crystallization Communications |
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Language: | English |
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2012
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ftdesyvdb:oai:bib-pubdb1.desy.de:141321 2023-05-15T16:08:48+02:00 Structural characterization of recombinant crustacyanin subunits from the lobster Homarus americanus Ferrari, M. Folli, C. Pincolini, E. McClintock, T. S. Roessle, M. Berni, R. Cianci, M. DE 2012 https://bib-pubdb1.desy.de/record/141321 https://bib-pubdb1.desy.de/search?p=id:%22PHPPUBDB-25469%22 eng eng Blackwell info:eu-repo/semantics/altIdentifier/pmid/pmid:22869108 info:eu-repo/semantics/altIdentifier/issn/1744-3091 info:eu-repo/semantics/altIdentifier/doi/10.1107/S1744309112026103 info:eu-repo/semantics/altIdentifier/wos/WOS:000307217700001 https://bib-pubdb1.desy.de/record/141321 https://bib-pubdb1.desy.de/search?p=id:%22PHPPUBDB-25469%22 info:eu-repo/semantics/openAccess Acta crystallographica / F 68, 846-853 (2012). doi:10.1107/S1744309112026103 info:eu-repo/classification/ddc/530 Amino Acid Sequence Animals Carrier Proteins: chemistry Conserved Sequence Models Molecular Molecular Sequence Data Nephropidae: chemistry Protein Structure Quaternary Tertiary Protein Subunits: chemistry Recombinant Proteins: chemistry Sequence Alignment Carrier Proteins Protein Subunits Recombinant Proteins crustacyanins info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2012 ftdesyvdb https://doi.org/10.1107/S1744309112026103 2022-06-30T20:09:37Z Crustacean crustacyanin proteins are linked to the production and modification of carapace colour, with direct implications for fitness and survival. Here, the structural and functional properties of the two recombinant crustacyanin subunits H(1) and H(2) from the American lobster Homarus americanus are reported. The two subunits are structurally highly similar to the corresponding natural apo crustacyanin CRTC and CRTA subunits from the European lobster H. gammarus. Reconstitution studies of the recombinant crustacyanin proteins H(1) and H(2) with astaxanthin reproduced the bathochromic shift of 85-95 nm typical of the natural crustacyanin subunits from H. gammarus in complex with astaxanthin. Moreover, correlations between the presence of crustacyanin genes in crustacean species and the resulting carapace colours with the spectral properties of the subunits in complex with astaxanthin confirmed this genotype-phenotype linkage. Article in Journal/Newspaper European lobster DESY Publication Database (PUBDB) Acta Crystallographica Section F Structural Biology and Crystallization Communications 68 8 846 853 |
institution |
Open Polar |
collection |
DESY Publication Database (PUBDB) |
op_collection_id |
ftdesyvdb |
language |
English |
topic |
info:eu-repo/classification/ddc/530 Amino Acid Sequence Animals Carrier Proteins: chemistry Conserved Sequence Models Molecular Molecular Sequence Data Nephropidae: chemistry Protein Structure Quaternary Tertiary Protein Subunits: chemistry Recombinant Proteins: chemistry Sequence Alignment Carrier Proteins Protein Subunits Recombinant Proteins crustacyanins |
spellingShingle |
info:eu-repo/classification/ddc/530 Amino Acid Sequence Animals Carrier Proteins: chemistry Conserved Sequence Models Molecular Molecular Sequence Data Nephropidae: chemistry Protein Structure Quaternary Tertiary Protein Subunits: chemistry Recombinant Proteins: chemistry Sequence Alignment Carrier Proteins Protein Subunits Recombinant Proteins crustacyanins Ferrari, M. Folli, C. Pincolini, E. McClintock, T. S. Roessle, M. Berni, R. Cianci, M. Structural characterization of recombinant crustacyanin subunits from the lobster Homarus americanus |
topic_facet |
info:eu-repo/classification/ddc/530 Amino Acid Sequence Animals Carrier Proteins: chemistry Conserved Sequence Models Molecular Molecular Sequence Data Nephropidae: chemistry Protein Structure Quaternary Tertiary Protein Subunits: chemistry Recombinant Proteins: chemistry Sequence Alignment Carrier Proteins Protein Subunits Recombinant Proteins crustacyanins |
description |
Crustacean crustacyanin proteins are linked to the production and modification of carapace colour, with direct implications for fitness and survival. Here, the structural and functional properties of the two recombinant crustacyanin subunits H(1) and H(2) from the American lobster Homarus americanus are reported. The two subunits are structurally highly similar to the corresponding natural apo crustacyanin CRTC and CRTA subunits from the European lobster H. gammarus. Reconstitution studies of the recombinant crustacyanin proteins H(1) and H(2) with astaxanthin reproduced the bathochromic shift of 85-95 nm typical of the natural crustacyanin subunits from H. gammarus in complex with astaxanthin. Moreover, correlations between the presence of crustacyanin genes in crustacean species and the resulting carapace colours with the spectral properties of the subunits in complex with astaxanthin confirmed this genotype-phenotype linkage. |
format |
Article in Journal/Newspaper |
author |
Ferrari, M. Folli, C. Pincolini, E. McClintock, T. S. Roessle, M. Berni, R. Cianci, M. |
author_facet |
Ferrari, M. Folli, C. Pincolini, E. McClintock, T. S. Roessle, M. Berni, R. Cianci, M. |
author_sort |
Ferrari, M. |
title |
Structural characterization of recombinant crustacyanin subunits from the lobster Homarus americanus |
title_short |
Structural characterization of recombinant crustacyanin subunits from the lobster Homarus americanus |
title_full |
Structural characterization of recombinant crustacyanin subunits from the lobster Homarus americanus |
title_fullStr |
Structural characterization of recombinant crustacyanin subunits from the lobster Homarus americanus |
title_full_unstemmed |
Structural characterization of recombinant crustacyanin subunits from the lobster Homarus americanus |
title_sort |
structural characterization of recombinant crustacyanin subunits from the lobster homarus americanus |
publisher |
Blackwell |
publishDate |
2012 |
url |
https://bib-pubdb1.desy.de/record/141321 https://bib-pubdb1.desy.de/search?p=id:%22PHPPUBDB-25469%22 |
op_coverage |
DE |
genre |
European lobster |
genre_facet |
European lobster |
op_source |
Acta crystallographica / F 68, 846-853 (2012). doi:10.1107/S1744309112026103 |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/pmid:22869108 info:eu-repo/semantics/altIdentifier/issn/1744-3091 info:eu-repo/semantics/altIdentifier/doi/10.1107/S1744309112026103 info:eu-repo/semantics/altIdentifier/wos/WOS:000307217700001 https://bib-pubdb1.desy.de/record/141321 https://bib-pubdb1.desy.de/search?p=id:%22PHPPUBDB-25469%22 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1107/S1744309112026103 |
container_title |
Acta Crystallographica Section F Structural Biology and Crystallization Communications |
container_volume |
68 |
container_issue |
8 |
container_start_page |
846 |
op_container_end_page |
853 |
_version_ |
1766404821684846592 |