Isolation and partial characterization of serum immunoglobuline in two species of sturgeons, Siberian sturgeon (Acipenser baerii) and Stellate sturgeon (Acipenser stellatus)

• Published in: Marine Science and Technology Bulletin
• Main Authors: Yektaseresht, Azadeh, Hemati, Zahra, Gholamhosseini, Amin
• Format: Article in Journal/Newspaper
• Language: English
• Published: Adem Yavuz SÖNMEZ 2018
• Subjects: Immunoglobulins,serum,Acipenser baerii,Acipenser stellatus; Acipenser baerii; Siberian sturgeon
• Online Access: https://dergipark.org.tr/tr/pub/masteb/issue/35587/375727; https://doi.org/10.33714/masteb.375727

The immunoglobulins (Igs) were isolated and purified respectively from serumof Siberian sturgeon (Acipenser baerii) and Stellate sturgeon (Acipenserstellatus) by using 50% saturated ammonium sulfate precipitation. Analysis of thepurified Ig of the two species of fishes on sodium dodecyl sulfate-polyacrylamidegel electrophoresis (SDS-PAGE) under reducing conditions revealed two bands,heavy chains and light chains respectively. The molecular weight of the heavychains of two fishes was about 70 kD and the molecular weight of the light chainswas 27kD for Siberian sturgeon and 27.5 kD for Stellate sturgeon. The resultssuggested that the isolated and purified serum Immunoglobuline in two species ofsturgeons, Siberian sturgeon (A. baerii) and Stellate sturgeon (A. stellatus) canbe used to produce polyclonal and monoclonal antibodies against sturgeon Ig fordeveloping diagnostic tests against a wide variety of pathogens.