Low oxygen stress during early development influences regulation of hypoxia-response genes in farmed atlantic salmon (Salmo salar)
Abstract Survival and growth of developing salmonids are negatively affected by low oxygen levels within gravel nests in natural streams, and hypoxic stress is often experienced by farmed Atlantic salmon (Salmo salar) within hatcheries. Exposure to hypoxia during early development may have long-last...
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ftdeakinunifig:oai:figshare.com:article/24418930 2024-06-23T07:51:14+00:00 Low oxygen stress during early development influences regulation of hypoxia-response genes in farmed atlantic salmon (Salmo salar) T Kelly H Johnsen E Burgerhout H Tveiten T Thesslund Ø Andersen Nick Robinson 2020-09-01T00:00:00Z http://hdl.handle.net/10779/DRO/DU:24418930.v2 https://figshare.com/articles/journal_contribution/Low_oxygen_stress_during_early_development_influences_regulation_of_hypoxia-response_genes_in_farmed_atlantic_salmon_Salmo_salar_/24418930 unknown http://hdl.handle.net/10779/DRO/DU:24418930.v2 https://figshare.com/articles/journal_contribution/Low_oxygen_stress_during_early_development_influences_regulation_of_hypoxia-response_genes_in_farmed_atlantic_salmon_Salmo_salar_/24418930 All Rights Reserved Biological sciences Biochemistry and cell biology Genetics Statistics DISSOLVED-OXYGEN DNA Methylation EPIGENETIC REGULATION EXPOSURE EXPRESSION gene regulation Genetics & Heredity GROWTH HEMOGLOBIN HIF-ALPHA hypoxia Life Sciences & Biomedicine METABOLISM prolyl hydroxylase domain enzymes salmon Science & Technology ZEBRAFISH Text Journal contribution 2020 ftdeakinunifig 2024-06-06T02:07:19Z Abstract Survival and growth of developing salmonids are negatively affected by low oxygen levels within gravel nests in natural streams, and hypoxic stress is often experienced by farmed Atlantic salmon (Salmo salar) within hatcheries. Exposure to hypoxia during early development may have long-lasting effects by altering epigenetic marks and gene expression in oxygen regulatory pathways. Here, we examine the transcriptomic response to low dissolved oxygen (DO) in post-hatch salmon reared continuously in 30%, 60% or 100% DO from fertilization until start of feeding. RNA sequencing revealed multiple differentially expressed genes, including oxygen transporting hemoglobin embryonic α subunit (hbae) and EGLN3 family hypoxia-inducible factor 3 (egln3) which regulates the stability of hypoxia inducible factor 1α (HIF-1α). Both hbae and egln3 displayed expression levels inversely correlated to oxygen concentration, and DNA methylation patterns within the egln3 promoter were negatively associated with the transcript levels. These results suggest that epigenetic processes are influenced by low oxygen levels during early development in Atlantic salmon to upregulate hypoxia-response genes. Article in Journal/Newspaper Atlantic salmon Salmo salar DRO - Deakin Research Online |
institution |
Open Polar |
collection |
DRO - Deakin Research Online |
op_collection_id |
ftdeakinunifig |
language |
unknown |
topic |
Biological sciences Biochemistry and cell biology Genetics Statistics DISSOLVED-OXYGEN DNA Methylation EPIGENETIC REGULATION EXPOSURE EXPRESSION gene regulation Genetics & Heredity GROWTH HEMOGLOBIN HIF-ALPHA hypoxia Life Sciences & Biomedicine METABOLISM prolyl hydroxylase domain enzymes salmon Science & Technology ZEBRAFISH |
spellingShingle |
Biological sciences Biochemistry and cell biology Genetics Statistics DISSOLVED-OXYGEN DNA Methylation EPIGENETIC REGULATION EXPOSURE EXPRESSION gene regulation Genetics & Heredity GROWTH HEMOGLOBIN HIF-ALPHA hypoxia Life Sciences & Biomedicine METABOLISM prolyl hydroxylase domain enzymes salmon Science & Technology ZEBRAFISH T Kelly H Johnsen E Burgerhout H Tveiten T Thesslund Ø Andersen Nick Robinson Low oxygen stress during early development influences regulation of hypoxia-response genes in farmed atlantic salmon (Salmo salar) |
topic_facet |
Biological sciences Biochemistry and cell biology Genetics Statistics DISSOLVED-OXYGEN DNA Methylation EPIGENETIC REGULATION EXPOSURE EXPRESSION gene regulation Genetics & Heredity GROWTH HEMOGLOBIN HIF-ALPHA hypoxia Life Sciences & Biomedicine METABOLISM prolyl hydroxylase domain enzymes salmon Science & Technology ZEBRAFISH |
description |
Abstract Survival and growth of developing salmonids are negatively affected by low oxygen levels within gravel nests in natural streams, and hypoxic stress is often experienced by farmed Atlantic salmon (Salmo salar) within hatcheries. Exposure to hypoxia during early development may have long-lasting effects by altering epigenetic marks and gene expression in oxygen regulatory pathways. Here, we examine the transcriptomic response to low dissolved oxygen (DO) in post-hatch salmon reared continuously in 30%, 60% or 100% DO from fertilization until start of feeding. RNA sequencing revealed multiple differentially expressed genes, including oxygen transporting hemoglobin embryonic α subunit (hbae) and EGLN3 family hypoxia-inducible factor 3 (egln3) which regulates the stability of hypoxia inducible factor 1α (HIF-1α). Both hbae and egln3 displayed expression levels inversely correlated to oxygen concentration, and DNA methylation patterns within the egln3 promoter were negatively associated with the transcript levels. These results suggest that epigenetic processes are influenced by low oxygen levels during early development in Atlantic salmon to upregulate hypoxia-response genes. |
format |
Article in Journal/Newspaper |
author |
T Kelly H Johnsen E Burgerhout H Tveiten T Thesslund Ø Andersen Nick Robinson |
author_facet |
T Kelly H Johnsen E Burgerhout H Tveiten T Thesslund Ø Andersen Nick Robinson |
author_sort |
T Kelly |
title |
Low oxygen stress during early development influences regulation of hypoxia-response genes in farmed atlantic salmon (Salmo salar) |
title_short |
Low oxygen stress during early development influences regulation of hypoxia-response genes in farmed atlantic salmon (Salmo salar) |
title_full |
Low oxygen stress during early development influences regulation of hypoxia-response genes in farmed atlantic salmon (Salmo salar) |
title_fullStr |
Low oxygen stress during early development influences regulation of hypoxia-response genes in farmed atlantic salmon (Salmo salar) |
title_full_unstemmed |
Low oxygen stress during early development influences regulation of hypoxia-response genes in farmed atlantic salmon (Salmo salar) |
title_sort |
low oxygen stress during early development influences regulation of hypoxia-response genes in farmed atlantic salmon (salmo salar) |
publishDate |
2020 |
url |
http://hdl.handle.net/10779/DRO/DU:24418930.v2 https://figshare.com/articles/journal_contribution/Low_oxygen_stress_during_early_development_influences_regulation_of_hypoxia-response_genes_in_farmed_atlantic_salmon_Salmo_salar_/24418930 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://hdl.handle.net/10779/DRO/DU:24418930.v2 https://figshare.com/articles/journal_contribution/Low_oxygen_stress_during_early_development_influences_regulation_of_hypoxia-response_genes_in_farmed_atlantic_salmon_Salmo_salar_/24418930 |
op_rights |
All Rights Reserved |
_version_ |
1802642268455174144 |