Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches
1. Understanding ecological phenomena often requires an accurate assessment of the timing of events. To estimate the time since a diet shift in animals without knowledge on the isotope ratios of either the old or the new diet, isotope ratio measurements in two different tissues (e.g. blood plasma an...
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ftdeakinunifig:oai:figshare.com:article/21010105 2023-05-15T15:48:30+02:00 Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches Marcel Klaassen T Piersma H Korthals A Dekinga M Dietz 2010-08-01T00:00:00Z http://hdl.handle.net/10536/DRO/DU:30035078 https://figshare.com/articles/journal_contribution/Single-point_isotope_measurements_in_blood_cells_and_plasma_to_estimate_the_time_since_diet_switches/21010105 unknown http://hdl.handle.net/10536/DRO/DU:30035078 https://figshare.com/articles/journal_contribution/Single-point_isotope_measurements_in_blood_cells_and_plasma_to_estimate_the_time_since_diet_switches/21010105 All Rights Reserved Uncategorized isotopic clock mathematical model sensitivity analysis stable carbon isotope timing of events Science & Technology Life Sciences & Biomedicine Ecology Environmental Sciences & Ecology CLIMATE-CHANGE N-15 INCORPORATION CARBON ISOTOPES TURNOVER C-13 REPRODUCTION DELTA-C-13 NITROGEN TISSUES SHIFTS Text Journal contribution 2010 ftdeakinunifig 2022-11-17T22:24:51Z 1. Understanding ecological phenomena often requires an accurate assessment of the timing of events. To estimate the time since a diet shift in animals without knowledge on the isotope ratios of either the old or the new diet, isotope ratio measurements in two different tissues (e.g. blood plasma and blood cells) at a single point in time can be used. For this ‘isotopic-clock’ principle, we present here a mathematical model that yields an analytical and easily calculated outcome. 2. Compared with a previously published model, our model assumes the isotopic difference between the old and new diets to be constant if multiple measurements are taken on the same subject at different points in time. Furthermore, to estimate the time since diet switch, no knowledge of the isotopic signature of tissues under the old diet, but only under the new diet is required. 3. The two models are compared using three calibration data sets including a novel one based on a diet shift experiment in a shorebird (red knot Calidris canutus); sensitivity analyses were conducted. The two models behaved differently and each may prove rather unsatisfactory depending on the system under investigation. A single-tissue model, requiring knowledge of both the old and new diets, generally behaved quite reliably. 4. As blood (cells) and plasma are particularly useful tissues for isotopic-clock research, we trawled the literature on turnover rates in whole blood, cells and plasma. Unfortunately, turnover rate predictions using allometric relations are too unreliable to be used directly in isotopic-clock calculations. 5. We advocate that before applying the isotopic-clock methodology, the propagation of error in the ‘time-since-diet-shift’ estimation is carefully assessed for the system under scrutiny using a sensitivity analysis as proposed here. Other Non-Article Part of Journal/Newspaper Calidris canutus Red Knot DRO - Deakin Research Online |
institution |
Open Polar |
collection |
DRO - Deakin Research Online |
op_collection_id |
ftdeakinunifig |
language |
unknown |
topic |
Uncategorized isotopic clock mathematical model sensitivity analysis stable carbon isotope timing of events Science & Technology Life Sciences & Biomedicine Ecology Environmental Sciences & Ecology CLIMATE-CHANGE N-15 INCORPORATION CARBON ISOTOPES TURNOVER C-13 REPRODUCTION DELTA-C-13 NITROGEN TISSUES SHIFTS |
spellingShingle |
Uncategorized isotopic clock mathematical model sensitivity analysis stable carbon isotope timing of events Science & Technology Life Sciences & Biomedicine Ecology Environmental Sciences & Ecology CLIMATE-CHANGE N-15 INCORPORATION CARBON ISOTOPES TURNOVER C-13 REPRODUCTION DELTA-C-13 NITROGEN TISSUES SHIFTS Marcel Klaassen T Piersma H Korthals A Dekinga M Dietz Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches |
topic_facet |
Uncategorized isotopic clock mathematical model sensitivity analysis stable carbon isotope timing of events Science & Technology Life Sciences & Biomedicine Ecology Environmental Sciences & Ecology CLIMATE-CHANGE N-15 INCORPORATION CARBON ISOTOPES TURNOVER C-13 REPRODUCTION DELTA-C-13 NITROGEN TISSUES SHIFTS |
description |
1. Understanding ecological phenomena often requires an accurate assessment of the timing of events. To estimate the time since a diet shift in animals without knowledge on the isotope ratios of either the old or the new diet, isotope ratio measurements in two different tissues (e.g. blood plasma and blood cells) at a single point in time can be used. For this ‘isotopic-clock’ principle, we present here a mathematical model that yields an analytical and easily calculated outcome. 2. Compared with a previously published model, our model assumes the isotopic difference between the old and new diets to be constant if multiple measurements are taken on the same subject at different points in time. Furthermore, to estimate the time since diet switch, no knowledge of the isotopic signature of tissues under the old diet, but only under the new diet is required. 3. The two models are compared using three calibration data sets including a novel one based on a diet shift experiment in a shorebird (red knot Calidris canutus); sensitivity analyses were conducted. The two models behaved differently and each may prove rather unsatisfactory depending on the system under investigation. A single-tissue model, requiring knowledge of both the old and new diets, generally behaved quite reliably. 4. As blood (cells) and plasma are particularly useful tissues for isotopic-clock research, we trawled the literature on turnover rates in whole blood, cells and plasma. Unfortunately, turnover rate predictions using allometric relations are too unreliable to be used directly in isotopic-clock calculations. 5. We advocate that before applying the isotopic-clock methodology, the propagation of error in the ‘time-since-diet-shift’ estimation is carefully assessed for the system under scrutiny using a sensitivity analysis as proposed here. |
format |
Other Non-Article Part of Journal/Newspaper |
author |
Marcel Klaassen T Piersma H Korthals A Dekinga M Dietz |
author_facet |
Marcel Klaassen T Piersma H Korthals A Dekinga M Dietz |
author_sort |
Marcel Klaassen |
title |
Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches |
title_short |
Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches |
title_full |
Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches |
title_fullStr |
Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches |
title_full_unstemmed |
Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches |
title_sort |
single-point isotope measurements in blood cells and plasma to estimate the time since diet switches |
publishDate |
2010 |
url |
http://hdl.handle.net/10536/DRO/DU:30035078 https://figshare.com/articles/journal_contribution/Single-point_isotope_measurements_in_blood_cells_and_plasma_to_estimate_the_time_since_diet_switches/21010105 |
genre |
Calidris canutus Red Knot |
genre_facet |
Calidris canutus Red Knot |
op_relation |
http://hdl.handle.net/10536/DRO/DU:30035078 https://figshare.com/articles/journal_contribution/Single-point_isotope_measurements_in_blood_cells_and_plasma_to_estimate_the_time_since_diet_switches/21010105 |
op_rights |
All Rights Reserved |
_version_ |
1766383466478305280 |