1H-NMR and mass spectrometric characterization of the metabolic response of juvenile Atlantic salmon (Salmo salar) to long-term handling stress
Stressors of various kinds constantly affect fish both in the wild and in culture, examples being acute water temperature and quality changes, predation, handling, and confinement. Known physiological responses of fish to stress such as increases in plasma cortisol and glucose levels, are considered...
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ftdeakinunifig:oai:figshare.com:article/20979712 2023-05-15T15:32:03+02:00 1H-NMR and mass spectrometric characterization of the metabolic response of juvenile Atlantic salmon (Salmo salar) to long-term handling stress T Karakach E Huenupi E Soo J Walter Luis Afonso 2009-03-01T00:00:00Z http://hdl.handle.net/10536/DRO/DU:30047877 https://figshare.com/articles/journal_contribution/1H-NMR_and_mass_spectrometric_characterization_of_the_metabolic_response_of_juvenile_Atlantic_salmon_Salmo_salar_to_long-term_handling_stress/20979712 unknown http://hdl.handle.net/10536/DRO/DU:30047877 https://figshare.com/articles/journal_contribution/1H-NMR_and_mass_spectrometric_characterization_of_the_metabolic_response_of_juvenile_Atlantic_salmon_Salmo_salar_to_long-term_handling_stress/20979712 All Rights Reserved Uncategorized 1H-NMR fish metabolomics salmo salar stress UPLC-MS Science & Technology Life Sciences & Biomedicine Endocrinology & Metabolism H-1-NMR TRIMETHYLAMINE OXIDE MAGNETIC-RESONANCE PLASMA-CORTISOL BREAST-CANCER NMR SPECTROSCOPY METABONOMICS RATS PHOSPHOCHOLINE Text Journal contribution 2009 ftdeakinunifig 2022-11-17T22:06:00Z Stressors of various kinds constantly affect fish both in the wild and in culture, examples being acute water temperature and quality changes, predation, handling, and confinement. Known physiological responses of fish to stress such as increases in plasma cortisol and glucose levels, are considered to be adaptive, allowing the animal to cope in the short term. Prolonged exposure to stressors however, has the potential to affect growth, immune function, and survival. Nonetheless, little is known about the mechanisms underlying the long-term stress response. We have investigated the metabolic response of juvenile Atlantic salmon (Salmo salar) to long-term handling stress by analyzing fish plasma via 1 H nuclear magnetic resonance spectroscopy and ultra high performance liquid chromatography–mass spectrometry (UPLC–MS), and comparing results with controls. Analysis of NMR data indicated a difference in the metabolic profiles of control and stressed fish after 1 week of stress with a maximum difference observed after 2 weeks. These differences were associated with stress-induced increases in phosphatidyl choline, lactate, carbohydrates, alanine, valine and trimethylamine-N-oxide, and decreases in low density lipoprotein, very low density lipoprotein, and lipid. UPLC-MS data showed differences at week 2, associated with another set of compounds, tentatively identified on the basis of their mass/charge. Overall the results provided a multi-faceted view of the response of fish to long-term handling stress, indicating that the metabolic disparity between the control and stress groups increased to week 2, but declined by weeks 3 and 4, and revealed several new molecular indicators of long-term stress. Other Non-Article Part of Journal/Newspaper Atlantic salmon Salmo salar DRO - Deakin Research Online |
institution |
Open Polar |
collection |
DRO - Deakin Research Online |
op_collection_id |
ftdeakinunifig |
language |
unknown |
topic |
Uncategorized 1H-NMR fish metabolomics salmo salar stress UPLC-MS Science & Technology Life Sciences & Biomedicine Endocrinology & Metabolism H-1-NMR TRIMETHYLAMINE OXIDE MAGNETIC-RESONANCE PLASMA-CORTISOL BREAST-CANCER NMR SPECTROSCOPY METABONOMICS RATS PHOSPHOCHOLINE |
spellingShingle |
Uncategorized 1H-NMR fish metabolomics salmo salar stress UPLC-MS Science & Technology Life Sciences & Biomedicine Endocrinology & Metabolism H-1-NMR TRIMETHYLAMINE OXIDE MAGNETIC-RESONANCE PLASMA-CORTISOL BREAST-CANCER NMR SPECTROSCOPY METABONOMICS RATS PHOSPHOCHOLINE T Karakach E Huenupi E Soo J Walter Luis Afonso 1H-NMR and mass spectrometric characterization of the metabolic response of juvenile Atlantic salmon (Salmo salar) to long-term handling stress |
topic_facet |
Uncategorized 1H-NMR fish metabolomics salmo salar stress UPLC-MS Science & Technology Life Sciences & Biomedicine Endocrinology & Metabolism H-1-NMR TRIMETHYLAMINE OXIDE MAGNETIC-RESONANCE PLASMA-CORTISOL BREAST-CANCER NMR SPECTROSCOPY METABONOMICS RATS PHOSPHOCHOLINE |
description |
Stressors of various kinds constantly affect fish both in the wild and in culture, examples being acute water temperature and quality changes, predation, handling, and confinement. Known physiological responses of fish to stress such as increases in plasma cortisol and glucose levels, are considered to be adaptive, allowing the animal to cope in the short term. Prolonged exposure to stressors however, has the potential to affect growth, immune function, and survival. Nonetheless, little is known about the mechanisms underlying the long-term stress response. We have investigated the metabolic response of juvenile Atlantic salmon (Salmo salar) to long-term handling stress by analyzing fish plasma via 1 H nuclear magnetic resonance spectroscopy and ultra high performance liquid chromatography–mass spectrometry (UPLC–MS), and comparing results with controls. Analysis of NMR data indicated a difference in the metabolic profiles of control and stressed fish after 1 week of stress with a maximum difference observed after 2 weeks. These differences were associated with stress-induced increases in phosphatidyl choline, lactate, carbohydrates, alanine, valine and trimethylamine-N-oxide, and decreases in low density lipoprotein, very low density lipoprotein, and lipid. UPLC-MS data showed differences at week 2, associated with another set of compounds, tentatively identified on the basis of their mass/charge. Overall the results provided a multi-faceted view of the response of fish to long-term handling stress, indicating that the metabolic disparity between the control and stress groups increased to week 2, but declined by weeks 3 and 4, and revealed several new molecular indicators of long-term stress. |
format |
Other Non-Article Part of Journal/Newspaper |
author |
T Karakach E Huenupi E Soo J Walter Luis Afonso |
author_facet |
T Karakach E Huenupi E Soo J Walter Luis Afonso |
author_sort |
T Karakach |
title |
1H-NMR and mass spectrometric characterization of the metabolic response of juvenile Atlantic salmon (Salmo salar) to long-term handling stress |
title_short |
1H-NMR and mass spectrometric characterization of the metabolic response of juvenile Atlantic salmon (Salmo salar) to long-term handling stress |
title_full |
1H-NMR and mass spectrometric characterization of the metabolic response of juvenile Atlantic salmon (Salmo salar) to long-term handling stress |
title_fullStr |
1H-NMR and mass spectrometric characterization of the metabolic response of juvenile Atlantic salmon (Salmo salar) to long-term handling stress |
title_full_unstemmed |
1H-NMR and mass spectrometric characterization of the metabolic response of juvenile Atlantic salmon (Salmo salar) to long-term handling stress |
title_sort |
1h-nmr and mass spectrometric characterization of the metabolic response of juvenile atlantic salmon (salmo salar) to long-term handling stress |
publishDate |
2009 |
url |
http://hdl.handle.net/10536/DRO/DU:30047877 https://figshare.com/articles/journal_contribution/1H-NMR_and_mass_spectrometric_characterization_of_the_metabolic_response_of_juvenile_Atlantic_salmon_Salmo_salar_to_long-term_handling_stress/20979712 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://hdl.handle.net/10536/DRO/DU:30047877 https://figshare.com/articles/journal_contribution/1H-NMR_and_mass_spectrometric_characterization_of_the_metabolic_response_of_juvenile_Atlantic_salmon_Salmo_salar_to_long-term_handling_stress/20979712 |
op_rights |
All Rights Reserved |
_version_ |
1766362554092748800 |