Taxon-specific responses of Southern Ocean diatoms to Fe enrichment revealed by synchrotron radiation FTIR microspectroscopy
Photosynthesis by marine diatoms contributes substantially to global biogeochemical cycling and ecosystem productivity. It is widely accepted that diatoms are extremely sensitive to changes in Fe availability, with numerous in situ experiments demonstrating rapid growth and increased export of eleme...
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ftdeakinunifig:oai:figshare.com:article/20908486 2024-06-23T07:56:55+00:00 Taxon-specific responses of Southern Ocean diatoms to Fe enrichment revealed by synchrotron radiation FTIR microspectroscopy O Sackett L Armand J Beardall R Hill M Doblin C Connelly J Howes B Stuart P Ralph P Heraud 2014-10-20T00:00:00Z http://hdl.handle.net/10536/DRO/DU:30072804 https://figshare.com/articles/journal_contribution/Taxon-specific_responses_of_Southern_Ocean_diatoms_to_Fe_enrichment_revealed_by_synchrotron_radiation_FTIR_microspectroscopy/20908486 unknown http://hdl.handle.net/10536/DRO/DU:30072804 https://figshare.com/articles/journal_contribution/Taxon-specific_responses_of_Southern_Ocean_diatoms_to_Fe_enrichment_revealed_by_synchrotron_radiation_FTIR_microspectroscopy/20908486 All Rights Reserved 050102 Ecosystem Function 059999 Environmental Sciences not elsewhere classified 030606 Structural Chemistry and Spectroscopy 970105 Expanding Knowledge in the Environmental Sciences School of Life and Environmental Sciences Text Journal contribution 2014 ftdeakinunifig 2024-06-12T23:58:10Z Photosynthesis by marine diatoms contributes substantially to global biogeochemical cycling and ecosystem productivity. It is widely accepted that diatoms are extremely sensitive to changes in Fe availability, with numerous in situ experiments demonstrating rapid growth and increased export of elements (e.g. C, Si and Fe) from surface waters as a result of Fe addition. Less is known about the effects of Fe enrichment on the phenotypes of diatoms, such as associated changes in nutritional value-furthermore, data on taxon-specific responses are almost non-existent. Enhanced supply of nutrient-rich waters along the coast of the subantarctic Kerguelen Island provide a valuable opportunity to examine the responses of phytoplankton to natural Fe enrichment. Here we demonstrate the use of synchrotron radiation Fourier Transform Infrared (SR-FTIR) microspectroscopy to analyse changes in the macromolecular composition of diatoms collected along the coast and plateau of Kerguelen Island, Southern Ocean. SR-FTIR microspectroscopy enabled the analysis of individual diatom cells from mixed communities of field-collected samples, thereby providing insight into in situ taxon-specific responses in relation to changes in Fe availability. Phenotypic responses were taxon-specific in terms of intraspecific variability and changes in proteins, amino acids, phosphorylated molecules, silicate/silicic acid and carbohydrates. In contrast to some previous studies, silicate/silicic acid levels increased under Fe enrichment, in conjunction with increases in carbohydrate stores. The highly abundant taxon Fragilariopsis kerguelensis displayed a higher level of phenotypic plasticity than Pseudo-nitzschia spp., while analysis of the data pooled across all measured taxa showed different patterns in macromolecular composition compared to those for individual taxon. This study demonstrates that taxon-specific responses to Fe enrichment may not always be accurately reflected by bulk community measurements, highlighting the need for further ... Article in Journal/Newspaper Southern Ocean DRO - Deakin Research Online Kerguelen Kerguelen Island ENVELOPE(69.500,69.500,-49.250,-49.250) Southern Ocean |
institution |
Open Polar |
collection |
DRO - Deakin Research Online |
op_collection_id |
ftdeakinunifig |
language |
unknown |
topic |
050102 Ecosystem Function 059999 Environmental Sciences not elsewhere classified 030606 Structural Chemistry and Spectroscopy 970105 Expanding Knowledge in the Environmental Sciences School of Life and Environmental Sciences |
spellingShingle |
050102 Ecosystem Function 059999 Environmental Sciences not elsewhere classified 030606 Structural Chemistry and Spectroscopy 970105 Expanding Knowledge in the Environmental Sciences School of Life and Environmental Sciences O Sackett L Armand J Beardall R Hill M Doblin C Connelly J Howes B Stuart P Ralph P Heraud Taxon-specific responses of Southern Ocean diatoms to Fe enrichment revealed by synchrotron radiation FTIR microspectroscopy |
topic_facet |
050102 Ecosystem Function 059999 Environmental Sciences not elsewhere classified 030606 Structural Chemistry and Spectroscopy 970105 Expanding Knowledge in the Environmental Sciences School of Life and Environmental Sciences |
description |
Photosynthesis by marine diatoms contributes substantially to global biogeochemical cycling and ecosystem productivity. It is widely accepted that diatoms are extremely sensitive to changes in Fe availability, with numerous in situ experiments demonstrating rapid growth and increased export of elements (e.g. C, Si and Fe) from surface waters as a result of Fe addition. Less is known about the effects of Fe enrichment on the phenotypes of diatoms, such as associated changes in nutritional value-furthermore, data on taxon-specific responses are almost non-existent. Enhanced supply of nutrient-rich waters along the coast of the subantarctic Kerguelen Island provide a valuable opportunity to examine the responses of phytoplankton to natural Fe enrichment. Here we demonstrate the use of synchrotron radiation Fourier Transform Infrared (SR-FTIR) microspectroscopy to analyse changes in the macromolecular composition of diatoms collected along the coast and plateau of Kerguelen Island, Southern Ocean. SR-FTIR microspectroscopy enabled the analysis of individual diatom cells from mixed communities of field-collected samples, thereby providing insight into in situ taxon-specific responses in relation to changes in Fe availability. Phenotypic responses were taxon-specific in terms of intraspecific variability and changes in proteins, amino acids, phosphorylated molecules, silicate/silicic acid and carbohydrates. In contrast to some previous studies, silicate/silicic acid levels increased under Fe enrichment, in conjunction with increases in carbohydrate stores. The highly abundant taxon Fragilariopsis kerguelensis displayed a higher level of phenotypic plasticity than Pseudo-nitzschia spp., while analysis of the data pooled across all measured taxa showed different patterns in macromolecular composition compared to those for individual taxon. This study demonstrates that taxon-specific responses to Fe enrichment may not always be accurately reflected by bulk community measurements, highlighting the need for further ... |
format |
Article in Journal/Newspaper |
author |
O Sackett L Armand J Beardall R Hill M Doblin C Connelly J Howes B Stuart P Ralph P Heraud |
author_facet |
O Sackett L Armand J Beardall R Hill M Doblin C Connelly J Howes B Stuart P Ralph P Heraud |
author_sort |
O Sackett |
title |
Taxon-specific responses of Southern Ocean diatoms to Fe enrichment revealed by synchrotron radiation FTIR microspectroscopy |
title_short |
Taxon-specific responses of Southern Ocean diatoms to Fe enrichment revealed by synchrotron radiation FTIR microspectroscopy |
title_full |
Taxon-specific responses of Southern Ocean diatoms to Fe enrichment revealed by synchrotron radiation FTIR microspectroscopy |
title_fullStr |
Taxon-specific responses of Southern Ocean diatoms to Fe enrichment revealed by synchrotron radiation FTIR microspectroscopy |
title_full_unstemmed |
Taxon-specific responses of Southern Ocean diatoms to Fe enrichment revealed by synchrotron radiation FTIR microspectroscopy |
title_sort |
taxon-specific responses of southern ocean diatoms to fe enrichment revealed by synchrotron radiation ftir microspectroscopy |
publishDate |
2014 |
url |
http://hdl.handle.net/10536/DRO/DU:30072804 https://figshare.com/articles/journal_contribution/Taxon-specific_responses_of_Southern_Ocean_diatoms_to_Fe_enrichment_revealed_by_synchrotron_radiation_FTIR_microspectroscopy/20908486 |
long_lat |
ENVELOPE(69.500,69.500,-49.250,-49.250) |
geographic |
Kerguelen Kerguelen Island Southern Ocean |
geographic_facet |
Kerguelen Kerguelen Island Southern Ocean |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_relation |
http://hdl.handle.net/10536/DRO/DU:30072804 https://figshare.com/articles/journal_contribution/Taxon-specific_responses_of_Southern_Ocean_diatoms_to_Fe_enrichment_revealed_by_synchrotron_radiation_FTIR_microspectroscopy/20908486 |
op_rights |
All Rights Reserved |
_version_ |
1802650316477300736 |