A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation
The colostrum trypsin inhibitor (CTI) gene and transcript were cloned from the Cape fur seal mammary gland and CTI identified by in silico analysis of the Pacific walrus and polar bear genomes (Order Carnivora), and in marine and terrestrial mammals of the Orders Cetartiodactyla (yak, whales, camel)...
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ftdeakinunifig:oai:figshare.com:article/20892694 2024-06-23T07:56:18+00:00 A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation EA Pharo KN Cane J McCoey AM Buckle WH Oosthuizen C Guinet John Arnould 2016-03-01T00:00:00Z http://hdl.handle.net/10536/DRO/DU:30080469 https://figshare.com/articles/journal_contribution/A_colostrum_trypsin_inhibitor_gene_expressed_in_the_Cape_fur_seal_mammary_gland_during_lactation/20892694 unknown http://hdl.handle.net/10536/DRO/DU:30080469 https://figshare.com/articles/journal_contribution/A_colostrum_trypsin_inhibitor_gene_expressed_in_the_Cape_fur_seal_mammary_gland_during_lactation/20892694 All Rights Reserved Genetics not elsewhere classified Animal physiology - biophysics Animal physiology - cell Animal physiology - systems Medical microbiology not elsewhere classified Science & Technology Life Sciences & Biomedicine Genetics & Heredity Pinniped Kunitz BPTI Protease Walrus Milk SERINE-PROTEASE ATTENDANCE PATTERNS MILK GLYCOBIOME KUNITZ DOMAIN IMPACT DIVERGENCE OLIGOSACCHARIDES IMMUNOGLOBULINS IMMUNOLOGY COMPLEXES 060405 Gene Expression (incl Microarray and other genome-wide approaches) 060205 Marine and Estuarine Ecology (incl Marine Ichthyology) 060407 Genome Structure and Regulation 970106 Expanding Knowledge in the Biological Sciences School of Life and Environmental Sciences 3105 Genetics 3109 Zoology Text Journal contribution 2016 ftdeakinunifig 2024-06-06T02:05:01Z The colostrum trypsin inhibitor (CTI) gene and transcript were cloned from the Cape fur seal mammary gland and CTI identified by in silico analysis of the Pacific walrus and polar bear genomes (Order Carnivora), and in marine and terrestrial mammals of the Orders Cetartiodactyla (yak, whales, camel) and Perissodactyla (white rhinoceros). Unexpectedly, Weddell seal CTI was predicted to be a pseudogene. Cape fur seal CTI was expressed in the mammary gland of a pregnant multiparous seal, but not in a seal in its first pregnancy. While bovine CTI is expressed for 24-48h postpartum (pp) and secreted in colostrum only, Cape fur seal CTI was detected for at least 2-3months pp while the mother was suckling its young on-shore. Furthermore, CTI was expressed in the mammary gland of only one of the lactating seals that was foraging at-sea. The expression of β-casein (CSN2) and β-lactoglobulin II (LGB2), but not CTI in the second lactating seal foraging at-sea suggested that CTI may be intermittently expressed during lactation. Cape fur seal and walrus CTI encode putative small, secreted, N-glycosylated proteins with a single Kunitz/bovine pancreatic trypsin inhibitor (BPTI) domain indicative of serine protease inhibition. Mature Cape fur seal CTI shares 92% sequence identity with Pacific walrus CTI, but only 35% identity with BPTI. Structural homology modelling of Cape fur seal CTI and Pacific walrus trypsin based on the model of the second Kunitz domain of human tissue factor pathway inhibitor (TFPI) and porcine trypsin (Protein Data Bank: 1TFX) confirmed that CTI inhibits trypsin in a canonical fashion. Therefore, pinniped CTI may be critical for preventing the proteolytic degradation of immunoglobulins that are passively transferred from mother to young via colostrum and milk. Article in Journal/Newspaper polar bear Weddell Seal walrus* DRO - Deakin Research Online Pacific Weddell |
institution |
Open Polar |
collection |
DRO - Deakin Research Online |
op_collection_id |
ftdeakinunifig |
language |
unknown |
topic |
Genetics not elsewhere classified Animal physiology - biophysics Animal physiology - cell Animal physiology - systems Medical microbiology not elsewhere classified Science & Technology Life Sciences & Biomedicine Genetics & Heredity Pinniped Kunitz BPTI Protease Walrus Milk SERINE-PROTEASE ATTENDANCE PATTERNS MILK GLYCOBIOME KUNITZ DOMAIN IMPACT DIVERGENCE OLIGOSACCHARIDES IMMUNOGLOBULINS IMMUNOLOGY COMPLEXES 060405 Gene Expression (incl Microarray and other genome-wide approaches) 060205 Marine and Estuarine Ecology (incl Marine Ichthyology) 060407 Genome Structure and Regulation 970106 Expanding Knowledge in the Biological Sciences School of Life and Environmental Sciences 3105 Genetics 3109 Zoology |
spellingShingle |
Genetics not elsewhere classified Animal physiology - biophysics Animal physiology - cell Animal physiology - systems Medical microbiology not elsewhere classified Science & Technology Life Sciences & Biomedicine Genetics & Heredity Pinniped Kunitz BPTI Protease Walrus Milk SERINE-PROTEASE ATTENDANCE PATTERNS MILK GLYCOBIOME KUNITZ DOMAIN IMPACT DIVERGENCE OLIGOSACCHARIDES IMMUNOGLOBULINS IMMUNOLOGY COMPLEXES 060405 Gene Expression (incl Microarray and other genome-wide approaches) 060205 Marine and Estuarine Ecology (incl Marine Ichthyology) 060407 Genome Structure and Regulation 970106 Expanding Knowledge in the Biological Sciences School of Life and Environmental Sciences 3105 Genetics 3109 Zoology EA Pharo KN Cane J McCoey AM Buckle WH Oosthuizen C Guinet John Arnould A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation |
topic_facet |
Genetics not elsewhere classified Animal physiology - biophysics Animal physiology - cell Animal physiology - systems Medical microbiology not elsewhere classified Science & Technology Life Sciences & Biomedicine Genetics & Heredity Pinniped Kunitz BPTI Protease Walrus Milk SERINE-PROTEASE ATTENDANCE PATTERNS MILK GLYCOBIOME KUNITZ DOMAIN IMPACT DIVERGENCE OLIGOSACCHARIDES IMMUNOGLOBULINS IMMUNOLOGY COMPLEXES 060405 Gene Expression (incl Microarray and other genome-wide approaches) 060205 Marine and Estuarine Ecology (incl Marine Ichthyology) 060407 Genome Structure and Regulation 970106 Expanding Knowledge in the Biological Sciences School of Life and Environmental Sciences 3105 Genetics 3109 Zoology |
description |
The colostrum trypsin inhibitor (CTI) gene and transcript were cloned from the Cape fur seal mammary gland and CTI identified by in silico analysis of the Pacific walrus and polar bear genomes (Order Carnivora), and in marine and terrestrial mammals of the Orders Cetartiodactyla (yak, whales, camel) and Perissodactyla (white rhinoceros). Unexpectedly, Weddell seal CTI was predicted to be a pseudogene. Cape fur seal CTI was expressed in the mammary gland of a pregnant multiparous seal, but not in a seal in its first pregnancy. While bovine CTI is expressed for 24-48h postpartum (pp) and secreted in colostrum only, Cape fur seal CTI was detected for at least 2-3months pp while the mother was suckling its young on-shore. Furthermore, CTI was expressed in the mammary gland of only one of the lactating seals that was foraging at-sea. The expression of β-casein (CSN2) and β-lactoglobulin II (LGB2), but not CTI in the second lactating seal foraging at-sea suggested that CTI may be intermittently expressed during lactation. Cape fur seal and walrus CTI encode putative small, secreted, N-glycosylated proteins with a single Kunitz/bovine pancreatic trypsin inhibitor (BPTI) domain indicative of serine protease inhibition. Mature Cape fur seal CTI shares 92% sequence identity with Pacific walrus CTI, but only 35% identity with BPTI. Structural homology modelling of Cape fur seal CTI and Pacific walrus trypsin based on the model of the second Kunitz domain of human tissue factor pathway inhibitor (TFPI) and porcine trypsin (Protein Data Bank: 1TFX) confirmed that CTI inhibits trypsin in a canonical fashion. Therefore, pinniped CTI may be critical for preventing the proteolytic degradation of immunoglobulins that are passively transferred from mother to young via colostrum and milk. |
format |
Article in Journal/Newspaper |
author |
EA Pharo KN Cane J McCoey AM Buckle WH Oosthuizen C Guinet John Arnould |
author_facet |
EA Pharo KN Cane J McCoey AM Buckle WH Oosthuizen C Guinet John Arnould |
author_sort |
EA Pharo |
title |
A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation |
title_short |
A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation |
title_full |
A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation |
title_fullStr |
A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation |
title_full_unstemmed |
A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation |
title_sort |
colostrum trypsin inhibitor gene expressed in the cape fur seal mammary gland during lactation |
publishDate |
2016 |
url |
http://hdl.handle.net/10536/DRO/DU:30080469 https://figshare.com/articles/journal_contribution/A_colostrum_trypsin_inhibitor_gene_expressed_in_the_Cape_fur_seal_mammary_gland_during_lactation/20892694 |
geographic |
Pacific Weddell |
geographic_facet |
Pacific Weddell |
genre |
polar bear Weddell Seal walrus* |
genre_facet |
polar bear Weddell Seal walrus* |
op_relation |
http://hdl.handle.net/10536/DRO/DU:30080469 https://figshare.com/articles/journal_contribution/A_colostrum_trypsin_inhibitor_gene_expressed_in_the_Cape_fur_seal_mammary_gland_during_lactation/20892694 |
op_rights |
All Rights Reserved |
_version_ |
1802649308709781504 |