Distribution and characterization of natriuretic peptide receptors in the gills of the spiny dogfish, Squalus acanthias
The distribution and nature of natriuretic peptide receptors (NPR) in the gills of dogfish, Squalus acanthias, were examined by tissue section autoradiography, competition analysis, protein electrophoresis, guanylate cyclase (GC) assays, and molecular cloning. Specific NP binding occurred on the gil...
Main Authors: | , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
1997
|
Subjects: | |
Online Access: | http://hdl.handle.net/10536/DRO/DU:30094397 https://figshare.com/articles/journal_contribution/Distribution_and_characterization_of_natriuretic_peptide_receptors_in_the_gills_of_the_spiny_dogfish_Squalus_acanthias/20868007 |
id |
ftdeakinunifig:oai:figshare.com:article/20868007 |
---|---|
record_format |
openpolar |
spelling |
ftdeakinunifig:oai:figshare.com:article/20868007 2024-06-23T07:57:33+00:00 Distribution and characterization of natriuretic peptide receptors in the gills of the spiny dogfish, Squalus acanthias John Donald T Toop DH Evans 1997-06-01T00:00:00Z http://hdl.handle.net/10536/DRO/DU:30094397 https://figshare.com/articles/journal_contribution/Distribution_and_characterization_of_natriuretic_peptide_receptors_in_the_gills_of_the_spiny_dogfish_Squalus_acanthias/20868007 unknown http://hdl.handle.net/10536/DRO/DU:30094397 https://figshare.com/articles/journal_contribution/Distribution_and_characterization_of_natriuretic_peptide_receptors_in_the_gills_of_the_spiny_dogfish_Squalus_acanthias/20868007 All Rights Reserved Veterinary sciences not elsewhere classified Animal physiology - biophysics Animal physiology - cell Animal physiology - systems Zoology not elsewhere classified Amino Acid Sequence Animals Aorta Abdominal Autoradiography Base Sequence Binding Sites Cloning Molecular Dogfish Electrophoresis Polyacrylamide Gel Gills Guanylate Cyclase Kinetics Ligands Molecular Sequence Data Molecular Weight Receptors Atrial Natriuretic Factor Salt Gland Sequence Alignment Swine Text Journal contribution 1997 ftdeakinunifig 2024-06-06T02:00:31Z The distribution and nature of natriuretic peptide receptors (NPR) in the gills of dogfish, Squalus acanthias, were examined by tissue section autoradiography, competition analysis, protein electrophoresis, guanylate cyclase (GC) assays, and molecular cloning. Specific NP binding occurred on the gill filaments, but not on the interbranchial septum or gill arch. The binding was densest on the efferent edge of the gills. Higher resolution light-microscopic examination of emulsion-coated sections showed that specific binding occurred mainly on the secondary lamellae and filament body and not on the arterial circulation. At least two types of NPR were revealed. One is linked to GC since NP binding stimulates the production of cGMP. The GC receptor may be similar to the NPR-B mammalian receptor since only pCNP stimulated cGMP production. The second receptor is not linked to GC and binds the specific ligand C-ANF [rat des(Gln18, Ser19, Gly20, Leu21, Gly22)]. The sequence of a cDNA generated using primers based on conserved regions of vertebrate NPR-C had considerable homology with mammalian and eel NPR-C and eel NPR-D. The presence of GC-linked NPR and NPR-C/ NPR-D suggests that the gills are an important target organ for NP action. Article in Journal/Newspaper spiny dogfish Squalus acanthias DRO - Deakin Research Online |
institution |
Open Polar |
collection |
DRO - Deakin Research Online |
op_collection_id |
ftdeakinunifig |
language |
unknown |
topic |
Veterinary sciences not elsewhere classified Animal physiology - biophysics Animal physiology - cell Animal physiology - systems Zoology not elsewhere classified Amino Acid Sequence Animals Aorta Abdominal Autoradiography Base Sequence Binding Sites Cloning Molecular Dogfish Electrophoresis Polyacrylamide Gel Gills Guanylate Cyclase Kinetics Ligands Molecular Sequence Data Molecular Weight Receptors Atrial Natriuretic Factor Salt Gland Sequence Alignment Swine |
spellingShingle |
Veterinary sciences not elsewhere classified Animal physiology - biophysics Animal physiology - cell Animal physiology - systems Zoology not elsewhere classified Amino Acid Sequence Animals Aorta Abdominal Autoradiography Base Sequence Binding Sites Cloning Molecular Dogfish Electrophoresis Polyacrylamide Gel Gills Guanylate Cyclase Kinetics Ligands Molecular Sequence Data Molecular Weight Receptors Atrial Natriuretic Factor Salt Gland Sequence Alignment Swine John Donald T Toop DH Evans Distribution and characterization of natriuretic peptide receptors in the gills of the spiny dogfish, Squalus acanthias |
topic_facet |
Veterinary sciences not elsewhere classified Animal physiology - biophysics Animal physiology - cell Animal physiology - systems Zoology not elsewhere classified Amino Acid Sequence Animals Aorta Abdominal Autoradiography Base Sequence Binding Sites Cloning Molecular Dogfish Electrophoresis Polyacrylamide Gel Gills Guanylate Cyclase Kinetics Ligands Molecular Sequence Data Molecular Weight Receptors Atrial Natriuretic Factor Salt Gland Sequence Alignment Swine |
description |
The distribution and nature of natriuretic peptide receptors (NPR) in the gills of dogfish, Squalus acanthias, were examined by tissue section autoradiography, competition analysis, protein electrophoresis, guanylate cyclase (GC) assays, and molecular cloning. Specific NP binding occurred on the gill filaments, but not on the interbranchial septum or gill arch. The binding was densest on the efferent edge of the gills. Higher resolution light-microscopic examination of emulsion-coated sections showed that specific binding occurred mainly on the secondary lamellae and filament body and not on the arterial circulation. At least two types of NPR were revealed. One is linked to GC since NP binding stimulates the production of cGMP. The GC receptor may be similar to the NPR-B mammalian receptor since only pCNP stimulated cGMP production. The second receptor is not linked to GC and binds the specific ligand C-ANF [rat des(Gln18, Ser19, Gly20, Leu21, Gly22)]. The sequence of a cDNA generated using primers based on conserved regions of vertebrate NPR-C had considerable homology with mammalian and eel NPR-C and eel NPR-D. The presence of GC-linked NPR and NPR-C/ NPR-D suggests that the gills are an important target organ for NP action. |
format |
Article in Journal/Newspaper |
author |
John Donald T Toop DH Evans |
author_facet |
John Donald T Toop DH Evans |
author_sort |
John Donald |
title |
Distribution and characterization of natriuretic peptide receptors in the gills of the spiny dogfish, Squalus acanthias |
title_short |
Distribution and characterization of natriuretic peptide receptors in the gills of the spiny dogfish, Squalus acanthias |
title_full |
Distribution and characterization of natriuretic peptide receptors in the gills of the spiny dogfish, Squalus acanthias |
title_fullStr |
Distribution and characterization of natriuretic peptide receptors in the gills of the spiny dogfish, Squalus acanthias |
title_full_unstemmed |
Distribution and characterization of natriuretic peptide receptors in the gills of the spiny dogfish, Squalus acanthias |
title_sort |
distribution and characterization of natriuretic peptide receptors in the gills of the spiny dogfish, squalus acanthias |
publishDate |
1997 |
url |
http://hdl.handle.net/10536/DRO/DU:30094397 https://figshare.com/articles/journal_contribution/Distribution_and_characterization_of_natriuretic_peptide_receptors_in_the_gills_of_the_spiny_dogfish_Squalus_acanthias/20868007 |
genre |
spiny dogfish Squalus acanthias |
genre_facet |
spiny dogfish Squalus acanthias |
op_relation |
http://hdl.handle.net/10536/DRO/DU:30094397 https://figshare.com/articles/journal_contribution/Distribution_and_characterization_of_natriuretic_peptide_receptors_in_the_gills_of_the_spiny_dogfish_Squalus_acanthias/20868007 |
op_rights |
All Rights Reserved |
_version_ |
1802651223083450368 |