A n-3 PUFA depletion applied to rainbow trout fry (Oncorhynchus mykiss) does not modulate its subsequent lipid bioconversion capacity

• Main Authors: J Mellery, J Brel, J Dort, F Geay, P Kestemont, David Francis, Y Larondelle, X Rollin
• Format: Other Non-Article Part of Journal/Newspaper
• Language: unknown
• Published: 2017
• Subjects: Uncategorized; ALA α-linolenic acid; CD coefficient of distance; DGC daily growth coefficient; FE feed efficiency; FO fish oil-based diet; LA linoleic acid; LC-PUFA long-chain PUFA; LO linseed oil-based diet; SO sunflower oil-based diet; SO\/FO SO until day 60 and then FO from days 61–96; SO\/LO SO until day 60 and then LO from days 61–96; Fatty acid metabolism; Lipid bioconversion capacity; Plant-derived oils; Rainbow trout; Whole body fatty acid balance method; Animal Feed; Animals; Aquaculture; Diet; Fatty Acid Desaturases; Fatty Acids; Omega-3; Omega-6; Fish Oils; Linseed Oil; Lipid Metabolism; Malnutrition; Oncorhynchus mykiss; Plant Oils; Science & Technology; Life Sciences & Biomedicine; Nutrition & Dietetics; FATTY-ACID-COMPOSITION; SALMO-SALAR L; DIETARY FISH-OIL; GROW-OUT; IN-VIVO; OXIDATIVE STABILITY; DESATURASE ACTIVITY; VEGETABLE-OILS; PROFILE CHANGE; ALA \n α-linolenic acid; Salmo salar
• Online Access: http://hdl.handle.net/10536/DRO/DU:30093032; https://figshare.com/articles/journal_contribution/A_n-3_PUFA_depletion_applied_to_rainbow_trout_fry_Oncorhynchus_mykiss_does_not_modulate_its_subsequent_lipid_bioconversion_capacity/20861719

Nutritional strategies are currently developed to produce farmed fish rich in n-3 long-chain PUFA (LC-PUFA) whilst replacing fish oil by plant-derived oils in aquafeeds. The optimisation of such strategies requires a thorough understanding of fish lipid metabolism and its nutritional modulation. The present study evaluated the fatty acid bioconversion capacity of rainbow trout (Oncorhynchus mykiss) fry previously depleted in n-3 PUFA through a 60-d pre-experimental feeding period with a sunflower oil-based diet (SO) followed by a 36-d experimental period during which fish were fed either a linseed oil-based diet (LO) (this treatment being called SO/LO) or a fish oil-based diet (FO) (this treatment being called SO/FO). These treatments were compared with fish continuously fed on SO, LO or FO for 96 d. At the end of the 36-d experimental period, SO/LO and SO/FO fish recovered >80 % of the n-3 LC-PUFA reported for LO and FO fish, respectively. Fish fed on LO showed high apparent in vivo elongation and desaturation activities along the n-3 biosynthesis pathway. However, at the end of the experimental period, no impact of the fish n-3 PUFA depletion was observed on apparent in vivo elongation and desaturation activities of SO/LO fish as compared with LO fish. In contrast, the fish n-3 PUFA depletion negatively modulated the n-6 PUFA bioconversion capacity of fish in terms of reduced apparent in vivo elongation and desaturation activities. The effects were similar after 10 or 36 d of the experimental period, indicating the absence of short-term effects.