Candida antarctica lipase A effectively concentrates DHA from fish and thraustochytrid oils
The fatty acid selectivity of Candida antarctica lipase A (CAL-A) was applied to produce DHA concentrate by controlling the rate and extent of hydrolysis. Calcium was utilized to achieve a higher degree of hydrolysis. CAL-A was not regioselective but rather fatty acid selective, showing sequential s...
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ftdeakinunifig:oai:figshare.com:article/20858161 2024-06-23T07:47:32+00:00 Candida antarctica lipase A effectively concentrates DHA from fish and thraustochytrid oils TO Akanbi Colin Barrow 2017-08-15T00:00:00Z http://hdl.handle.net/10536/DRO/DU:30092149 https://figshare.com/articles/journal_contribution/Candida_antarctica_lipase_A_effectively_concentrates_DHA_from_fish_and_thraustochytrid_oils/20858161 unknown http://hdl.handle.net/10536/DRO/DU:30092149 https://figshare.com/articles/journal_contribution/Candida_antarctica_lipase_A_effectively_concentrates_DHA_from_fish_and_thraustochytrid_oils/20858161 All Rights Reserved Analytical chemistry not elsewhere classified CAL-A Concentration DHA Lipase Omega-3 Thraustochytrid Animals Candida Docosahexaenoic Acids Eurotiales Fishes Fungal Proteins Hydrolysis Oils Stramenopiles Substrate Specificity Centre for Chemistry and Biotechnology 030199 Analytical Chemistry not elsewhere classified MD Multidisciplinary 3401 Analytical chemistry Text Journal contribution 2017 ftdeakinunifig 2024-06-06T01:59:03Z The fatty acid selectivity of Candida antarctica lipase A (CAL-A) was applied to produce DHA concentrate by controlling the rate and extent of hydrolysis. Calcium was utilized to achieve a higher degree of hydrolysis. CAL-A was not regioselective but rather fatty acid selective, showing sequential selectivity for saturated, monounsaturated and polyunsaturated fatty acids in the order of increasing double bonds. Based on its strong initial preference for saturates, CAL-A was used to concentrate 82% docosahexaenoic acid (DHA) and 11% omega-6 docosapentaenoic acid (DPA-n6) after partial hydrolysis of algal oil. Thermomyces lanuginosus (TL 100L) lipase was used to partially remove DPA-n6, further concentrating DHA to 89%. CAL-A was immobilized on octadecyl-activated resin without altering its fatty acid selectivity. Article in Journal/Newspaper Antarc* Antarctica DRO - Deakin Research Online |
institution |
Open Polar |
collection |
DRO - Deakin Research Online |
op_collection_id |
ftdeakinunifig |
language |
unknown |
topic |
Analytical chemistry not elsewhere classified CAL-A Concentration DHA Lipase Omega-3 Thraustochytrid Animals Candida Docosahexaenoic Acids Eurotiales Fishes Fungal Proteins Hydrolysis Oils Stramenopiles Substrate Specificity Centre for Chemistry and Biotechnology 030199 Analytical Chemistry not elsewhere classified MD Multidisciplinary 3401 Analytical chemistry |
spellingShingle |
Analytical chemistry not elsewhere classified CAL-A Concentration DHA Lipase Omega-3 Thraustochytrid Animals Candida Docosahexaenoic Acids Eurotiales Fishes Fungal Proteins Hydrolysis Oils Stramenopiles Substrate Specificity Centre for Chemistry and Biotechnology 030199 Analytical Chemistry not elsewhere classified MD Multidisciplinary 3401 Analytical chemistry TO Akanbi Colin Barrow Candida antarctica lipase A effectively concentrates DHA from fish and thraustochytrid oils |
topic_facet |
Analytical chemistry not elsewhere classified CAL-A Concentration DHA Lipase Omega-3 Thraustochytrid Animals Candida Docosahexaenoic Acids Eurotiales Fishes Fungal Proteins Hydrolysis Oils Stramenopiles Substrate Specificity Centre for Chemistry and Biotechnology 030199 Analytical Chemistry not elsewhere classified MD Multidisciplinary 3401 Analytical chemistry |
description |
The fatty acid selectivity of Candida antarctica lipase A (CAL-A) was applied to produce DHA concentrate by controlling the rate and extent of hydrolysis. Calcium was utilized to achieve a higher degree of hydrolysis. CAL-A was not regioselective but rather fatty acid selective, showing sequential selectivity for saturated, monounsaturated and polyunsaturated fatty acids in the order of increasing double bonds. Based on its strong initial preference for saturates, CAL-A was used to concentrate 82% docosahexaenoic acid (DHA) and 11% omega-6 docosapentaenoic acid (DPA-n6) after partial hydrolysis of algal oil. Thermomyces lanuginosus (TL 100L) lipase was used to partially remove DPA-n6, further concentrating DHA to 89%. CAL-A was immobilized on octadecyl-activated resin without altering its fatty acid selectivity. |
format |
Article in Journal/Newspaper |
author |
TO Akanbi Colin Barrow |
author_facet |
TO Akanbi Colin Barrow |
author_sort |
TO Akanbi |
title |
Candida antarctica lipase A effectively concentrates DHA from fish and thraustochytrid oils |
title_short |
Candida antarctica lipase A effectively concentrates DHA from fish and thraustochytrid oils |
title_full |
Candida antarctica lipase A effectively concentrates DHA from fish and thraustochytrid oils |
title_fullStr |
Candida antarctica lipase A effectively concentrates DHA from fish and thraustochytrid oils |
title_full_unstemmed |
Candida antarctica lipase A effectively concentrates DHA from fish and thraustochytrid oils |
title_sort |
candida antarctica lipase a effectively concentrates dha from fish and thraustochytrid oils |
publishDate |
2017 |
url |
http://hdl.handle.net/10536/DRO/DU:30092149 https://figshare.com/articles/journal_contribution/Candida_antarctica_lipase_A_effectively_concentrates_DHA_from_fish_and_thraustochytrid_oils/20858161 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://hdl.handle.net/10536/DRO/DU:30092149 https://figshare.com/articles/journal_contribution/Candida_antarctica_lipase_A_effectively_concentrates_DHA_from_fish_and_thraustochytrid_oils/20858161 |
op_rights |
All Rights Reserved |
_version_ |
1802651660839813120 |