Candida antarctica lipase A effectively concentrates DHA from fish and thraustochytrid oils

The fatty acid selectivity of Candida antarctica lipase A (CAL-A) was applied to produce DHA concentrate by controlling the rate and extent of hydrolysis. Calcium was utilized to achieve a higher degree of hydrolysis. CAL-A was not regioselective but rather fatty acid selective, showing sequential s...

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Bibliographic Details
Main Authors: TO Akanbi, Colin Barrow
Format: Article in Journal/Newspaper
Language:unknown
Published: 2017
Subjects:
Analytical chemistry not elsewhere classified
CAL-A
Concentration
DHA
Lipase
Omega-3
Thraustochytrid
Animals
Candida
Docosahexaenoic Acids
Eurotiales
Fishes
Fungal Proteins
Hydrolysis
Oils
Stramenopiles
Substrate Specificity
Centre for Chemistry and Biotechnology
030199 Analytical Chemistry not elsewhere classified
MD Multidisciplinary
3401 Analytical chemistry
Antarc*
Antarctica
Online Access:http://hdl.handle.net/10536/DRO/DU:30092149
https://figshare.com/articles/journal_contribution/Candida_antarctica_lipase_A_effectively_concentrates_DHA_from_fish_and_thraustochytrid_oils/20858161
Description
Summary:The fatty acid selectivity of Candida antarctica lipase A (CAL-A) was applied to produce DHA concentrate by controlling the rate and extent of hydrolysis. Calcium was utilized to achieve a higher degree of hydrolysis. CAL-A was not regioselective but rather fatty acid selective, showing sequential selectivity for saturated, monounsaturated and polyunsaturated fatty acids in the order of increasing double bonds. Based on its strong initial preference for saturates, CAL-A was used to concentrate 82% docosahexaenoic acid (DHA) and 11% omega-6 docosapentaenoic acid (DPA-n6) after partial hydrolysis of algal oil. Thermomyces lanuginosus (TL 100L) lipase was used to partially remove DPA-n6, further concentrating DHA to 89%. CAL-A was immobilized on octadecyl-activated resin without altering its fatty acid selectivity.

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