Characterisation of proteins in the milk of fur seals
Milk protein composition was investigated throughout the lactation periods of the Australian fur seal ( Arctocephalus pusillus doriferus ) and Antarctic fur seal ( Arctocephalus gazella ). The mean protein content of the milk was found to be 10.9% and 10.6% respectively. The concentration of total p...
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ftdeakinunifig:oai:figshare.com:article/20559402 2023-05-15T14:05:21+02:00 Characterisation of proteins in the milk of fur seals K Cane John Arnould Kevin Nicholas 2005-05-01T00:00:00Z http://hdl.handle.net/10536/DRO/DU:30008863 https://figshare.com/articles/journal_contribution/Characterisation_of_proteins_in_the_milk_of_fur_seals/20559402 unknown http://hdl.handle.net/10536/DRO/DU:30008863 https://figshare.com/articles/journal_contribution/Characterisation_of_proteins_in_the_milk_of_fur_seals/20559402 All Rights Reserved Physiology beta-lactoglobulin casein fur seal lactation milk composition protein sequence whey Science & Technology Life Sciences & Biomedicine Biochemistry & Molecular Biology Zoology BOVINE BETA-LACTOGLOBULIN SOUTHERN ELEPHANT SEAL FATTY-ACID-COMPOSITION WHEY PROTEINS SECRETION RETINOL BINDING IDENTIFICATION SUPERFAMILY Text Journal contribution 2005 ftdeakinunifig 2022-11-17T23:08:51Z Milk protein composition was investigated throughout the lactation periods of the Australian fur seal ( Arctocephalus pusillus doriferus ) and Antarctic fur seal ( Arctocephalus gazella ). The mean protein content of the milk was found to be 10.9% and 10.6% respectively. The concentration of total protein did not change during lactation, although a decline in casein content of the milk in late lactation was apparent. Milk protein concentration during a foraging/suckling cycle of the Antarctic fur seal analysed at the time of arrival on shore, and 24 h and 72 h after arrival was 12.8%, 11.4% and 12.5% respectively. Re-feeding animals at 72 h resulted in a significant increase in milk protein content to 14.9%. Characterisation of milk protein by SDS-PAGE analysis revealed 5 casein and 10 major whey protein bands. Amino-terminal sequencing indicated that the majority of the whey fraction of the milk is β-lactoglobulin (β-LG). The limited amino acid sequence indicated 3 different β-LGs were secreted in the milk. Subsequently, RT-PCR was used to extend the sequence of one of the β-LGs and translation of the 464 bp fragment indicated that it shared 79% sequence identity with feline β-LG II. Other Non-Article Part of Journal/Newspaper Antarc* Antarctic Antarctic Fur Seal Arctocephalus gazella Elephant Seal Southern Elephant Seal DRO - Deakin Research Online Antarctic The Antarctic |
institution |
Open Polar |
collection |
DRO - Deakin Research Online |
op_collection_id |
ftdeakinunifig |
language |
unknown |
topic |
Physiology beta-lactoglobulin casein fur seal lactation milk composition protein sequence whey Science & Technology Life Sciences & Biomedicine Biochemistry & Molecular Biology Zoology BOVINE BETA-LACTOGLOBULIN SOUTHERN ELEPHANT SEAL FATTY-ACID-COMPOSITION WHEY PROTEINS SECRETION RETINOL BINDING IDENTIFICATION SUPERFAMILY |
spellingShingle |
Physiology beta-lactoglobulin casein fur seal lactation milk composition protein sequence whey Science & Technology Life Sciences & Biomedicine Biochemistry & Molecular Biology Zoology BOVINE BETA-LACTOGLOBULIN SOUTHERN ELEPHANT SEAL FATTY-ACID-COMPOSITION WHEY PROTEINS SECRETION RETINOL BINDING IDENTIFICATION SUPERFAMILY K Cane John Arnould Kevin Nicholas Characterisation of proteins in the milk of fur seals |
topic_facet |
Physiology beta-lactoglobulin casein fur seal lactation milk composition protein sequence whey Science & Technology Life Sciences & Biomedicine Biochemistry & Molecular Biology Zoology BOVINE BETA-LACTOGLOBULIN SOUTHERN ELEPHANT SEAL FATTY-ACID-COMPOSITION WHEY PROTEINS SECRETION RETINOL BINDING IDENTIFICATION SUPERFAMILY |
description |
Milk protein composition was investigated throughout the lactation periods of the Australian fur seal ( Arctocephalus pusillus doriferus ) and Antarctic fur seal ( Arctocephalus gazella ). The mean protein content of the milk was found to be 10.9% and 10.6% respectively. The concentration of total protein did not change during lactation, although a decline in casein content of the milk in late lactation was apparent. Milk protein concentration during a foraging/suckling cycle of the Antarctic fur seal analysed at the time of arrival on shore, and 24 h and 72 h after arrival was 12.8%, 11.4% and 12.5% respectively. Re-feeding animals at 72 h resulted in a significant increase in milk protein content to 14.9%. Characterisation of milk protein by SDS-PAGE analysis revealed 5 casein and 10 major whey protein bands. Amino-terminal sequencing indicated that the majority of the whey fraction of the milk is β-lactoglobulin (β-LG). The limited amino acid sequence indicated 3 different β-LGs were secreted in the milk. Subsequently, RT-PCR was used to extend the sequence of one of the β-LGs and translation of the 464 bp fragment indicated that it shared 79% sequence identity with feline β-LG II. |
format |
Other Non-Article Part of Journal/Newspaper |
author |
K Cane John Arnould Kevin Nicholas |
author_facet |
K Cane John Arnould Kevin Nicholas |
author_sort |
K Cane |
title |
Characterisation of proteins in the milk of fur seals |
title_short |
Characterisation of proteins in the milk of fur seals |
title_full |
Characterisation of proteins in the milk of fur seals |
title_fullStr |
Characterisation of proteins in the milk of fur seals |
title_full_unstemmed |
Characterisation of proteins in the milk of fur seals |
title_sort |
characterisation of proteins in the milk of fur seals |
publishDate |
2005 |
url |
http://hdl.handle.net/10536/DRO/DU:30008863 https://figshare.com/articles/journal_contribution/Characterisation_of_proteins_in_the_milk_of_fur_seals/20559402 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctic Fur Seal Arctocephalus gazella Elephant Seal Southern Elephant Seal |
genre_facet |
Antarc* Antarctic Antarctic Fur Seal Arctocephalus gazella Elephant Seal Southern Elephant Seal |
op_relation |
http://hdl.handle.net/10536/DRO/DU:30008863 https://figshare.com/articles/journal_contribution/Characterisation_of_proteins_in_the_milk_of_fur_seals/20559402 |
op_rights |
All Rights Reserved |
_version_ |
1766277180594061312 |