Role of MMF1 Gene in Mannosylerythritol Lipid Secretion and Sustainable Biosurfactant Production from Brackish Water ...

• Main Author: Xu, Yuze
• Format: Text
• Language: unknown
• Published: University of Alberta Library 2023
• Subjects: Antarc*; Antarctica
• Online Access: https://dx.doi.org/10.7939/r3-5s8x-3h20; https://era.library.ualberta.ca/items/e53922d4-53ea-4ea6-940e-3dce8953e033

This thesis investigates the secretion of mannosylerythritol lipid (MEL) in the JCM10317 strain of P. antarctica, and the potential for a more cost-effective, environmentally friendly production process for MEL. The study demonstrates that the Pa MMF1 gene is necessary for the secretion of di-acylated MEL-A/B/C when utilizing water-soluble carbon sources, and also identifies an unknown species of MEL. In addition, the amount of MEL in the cellular fraction of the knockout strain is equal to or more than that of the wild-type strain. A MEL species shift towards more hydrophobic MEL-A was also observed in the cellular fraction of MMF1 KO groups. This is the first study to investigate the relationship between the MMF1 gene and MEL secretion in water-soluble carbon sources, providing new insights into the mechanisms of MEL secretion. In the brackish water production experiment, the findings demonstrate that the JCM10317 strain can ferment MEL with unsterilized brackish water as a production medium, with waste ...