First Molecular Evidence for Puumala Hantavirus in Poland
Puumala virus (PUUV) causes mild to moderate cases of haemorrhagic fever with renal syndrome (HFRS), and is responsible for the majority of hantavirus infections of humans in Fennoscandia, Central and Western Europe. Although there are relatively many PUUV sequences available from different European...
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ftdatacite:10.7892/boris.71408 2023-05-15T16:11:58+02:00 First Molecular Evidence for Puumala Hantavirus in Poland Sadowska, Edyta T. Drewes, Stephan Mikowska, Magdalena Groschup, Martin H. Sheikh Ali, Hanan Koteja, Pawel Heckel, Gerald Ulrich, Rainer G. 2014 application/pdf https://dx.doi.org/10.7892/boris.71408 http://boris.unibe.ch/71408/ en eng Molecular Diversity Preservation International MDPI info:eu-repo/semantics/openAccess 570 Life sciences; biology CreativeWork article 2014 ftdatacite https://doi.org/10.7892/boris.71408 2021-11-05T12:55:41Z Puumala virus (PUUV) causes mild to moderate cases of haemorrhagic fever with renal syndrome (HFRS), and is responsible for the majority of hantavirus infections of humans in Fennoscandia, Central and Western Europe. Although there are relatively many PUUV sequences available from different European countries, little is known about the presence of this virus in Poland. During population studies in 2009 a total of 45 bank voles were trapped at three sites in north-eastern Poland, namely islands on Dejguny and Dobskie Lakes and in a forest near Mikołajki. S and M segment-specific RT-PCR assays detected PUUV RNA in three animals from the Mikołajki site. The obtained partial S and M segment sequences demonstrated the highest similarity to the corresponding segments of a PUUV strain from Latvia. Analysis of chest cavity fluid samples by IgG ELISA using a yeast-expressed PUUV nucleocapsid protein resulted in the detection of two seropositive samples, both being also RT-PCR positive. Interestingly, at the trapping site in Mikołajki PUUV-positive bank voles belong to the Carpathian and Eastern genetic lineages within this species. In conclusion, we herein present the first molecular evidence for PUUV in the rodent reservoir from Poland. Article in Journal/Newspaper Fennoscandia DataCite Metadata Store (German National Library of Science and Technology) |
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English |
topic |
570 Life sciences; biology |
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570 Life sciences; biology Sadowska, Edyta T. Drewes, Stephan Mikowska, Magdalena Groschup, Martin H. Sheikh Ali, Hanan Koteja, Pawel Heckel, Gerald Ulrich, Rainer G. First Molecular Evidence for Puumala Hantavirus in Poland |
topic_facet |
570 Life sciences; biology |
description |
Puumala virus (PUUV) causes mild to moderate cases of haemorrhagic fever with renal syndrome (HFRS), and is responsible for the majority of hantavirus infections of humans in Fennoscandia, Central and Western Europe. Although there are relatively many PUUV sequences available from different European countries, little is known about the presence of this virus in Poland. During population studies in 2009 a total of 45 bank voles were trapped at three sites in north-eastern Poland, namely islands on Dejguny and Dobskie Lakes and in a forest near Mikołajki. S and M segment-specific RT-PCR assays detected PUUV RNA in three animals from the Mikołajki site. The obtained partial S and M segment sequences demonstrated the highest similarity to the corresponding segments of a PUUV strain from Latvia. Analysis of chest cavity fluid samples by IgG ELISA using a yeast-expressed PUUV nucleocapsid protein resulted in the detection of two seropositive samples, both being also RT-PCR positive. Interestingly, at the trapping site in Mikołajki PUUV-positive bank voles belong to the Carpathian and Eastern genetic lineages within this species. In conclusion, we herein present the first molecular evidence for PUUV in the rodent reservoir from Poland. |
format |
Article in Journal/Newspaper |
author |
Sadowska, Edyta T. Drewes, Stephan Mikowska, Magdalena Groschup, Martin H. Sheikh Ali, Hanan Koteja, Pawel Heckel, Gerald Ulrich, Rainer G. |
author_facet |
Sadowska, Edyta T. Drewes, Stephan Mikowska, Magdalena Groschup, Martin H. Sheikh Ali, Hanan Koteja, Pawel Heckel, Gerald Ulrich, Rainer G. |
author_sort |
Sadowska, Edyta T. |
title |
First Molecular Evidence for Puumala Hantavirus in Poland |
title_short |
First Molecular Evidence for Puumala Hantavirus in Poland |
title_full |
First Molecular Evidence for Puumala Hantavirus in Poland |
title_fullStr |
First Molecular Evidence for Puumala Hantavirus in Poland |
title_full_unstemmed |
First Molecular Evidence for Puumala Hantavirus in Poland |
title_sort |
first molecular evidence for puumala hantavirus in poland |
publisher |
Molecular Diversity Preservation International MDPI |
publishDate |
2014 |
url |
https://dx.doi.org/10.7892/boris.71408 http://boris.unibe.ch/71408/ |
genre |
Fennoscandia |
genre_facet |
Fennoscandia |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.7892/boris.71408 |
_version_ |
1765997181895966720 |