Changing lanes : seasonal differences in energy metabolism of adipocytes in grizzly bears (Ursus arctos horribilis)
Obesity is among the most prevalent of health conditions in humans leading to a multitude of : metabolic pathologies such as type 2 diabetes and hyperglycemia. However, there are many wild : animals that have large seasonal cycles of fat accumulation and loss that do not exhibit the health : consequ...
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ftdatacite:10.7273/000000056 2023-05-15T18:41:58+02:00 Changing lanes : seasonal differences in energy metabolism of adipocytes in grizzly bears (Ursus arctos horribilis) Hapner, Hannah 2022 application/pdf https://dx.doi.org/10.7273/000000056 https://rex.libraries.wsu.edu/esploro/outputs/graduate/99900587063801842 en eng Washington State University Embargo Master thesis ScholarlyArticle Text article-journal 2022 ftdatacite https://doi.org/10.7273/000000056 2022-03-10T11:01:47Z Obesity is among the most prevalent of health conditions in humans leading to a multitude of : metabolic pathologies such as type 2 diabetes and hyperglycemia. However, there are many wild : animals that have large seasonal cycles of fat accumulation and loss that do not exhibit the health : consequences observed in obese humans. One example is the grizzly bear (Ursus arctos : horribilis) that can have body fat contents >40%. It is known that hibernating bears survive by : metabolizing their fat stores. Previous in vitro studies found that hibernation season adipocytes : are insulin resistant and exhibit increased lipolysis as measured by extracellular glycerol. Yet, : other aspects of cellular metabolism were not addressed, leaving this in vitro model incomplete. : To this end, the current studies were performed to determine if the cellular energetic phenotype - : measured via metabolic flux - of hibernating bears was retained in cultured adipocytes and to : what extent that was due to serum or intrinsic cellular factors. Extracellular acidification rate : (ECAR) and oxygen consumption rate (OCR) were used to calculate proton efflux rate (PER), : and total ATP defined as both ATP from glycolysis and from mitochondrial respiration. : Hibernation serum treated adipocytes lack metabolic flexibility and produce less ATP than active : serum treated adipocytes. Insulin had minor influence on ATP production, but inhibited lipolysis in active but not hibernation serum treated adipocytes. This suggests that the reduction in : glycolysis during hibernation is occurring downstream of insulin signaling and glucose uptake. : These findings reveal a potent seasonal serum effect on metabolic capacity of bear adipocytes. : Elucidation of responsible serum components involved and the cellular mechanisms that enable : these influences may provide a novel avenue for the development of future treatments of human : metabolic diseases. Text Ursus arctos DataCite Metadata Store (German National Library of Science and Technology) Lanes ENVELOPE(18.933,18.933,69.617,69.617) |
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English |
description |
Obesity is among the most prevalent of health conditions in humans leading to a multitude of : metabolic pathologies such as type 2 diabetes and hyperglycemia. However, there are many wild : animals that have large seasonal cycles of fat accumulation and loss that do not exhibit the health : consequences observed in obese humans. One example is the grizzly bear (Ursus arctos : horribilis) that can have body fat contents >40%. It is known that hibernating bears survive by : metabolizing their fat stores. Previous in vitro studies found that hibernation season adipocytes : are insulin resistant and exhibit increased lipolysis as measured by extracellular glycerol. Yet, : other aspects of cellular metabolism were not addressed, leaving this in vitro model incomplete. : To this end, the current studies were performed to determine if the cellular energetic phenotype - : measured via metabolic flux - of hibernating bears was retained in cultured adipocytes and to : what extent that was due to serum or intrinsic cellular factors. Extracellular acidification rate : (ECAR) and oxygen consumption rate (OCR) were used to calculate proton efflux rate (PER), : and total ATP defined as both ATP from glycolysis and from mitochondrial respiration. : Hibernation serum treated adipocytes lack metabolic flexibility and produce less ATP than active : serum treated adipocytes. Insulin had minor influence on ATP production, but inhibited lipolysis in active but not hibernation serum treated adipocytes. This suggests that the reduction in : glycolysis during hibernation is occurring downstream of insulin signaling and glucose uptake. : These findings reveal a potent seasonal serum effect on metabolic capacity of bear adipocytes. : Elucidation of responsible serum components involved and the cellular mechanisms that enable : these influences may provide a novel avenue for the development of future treatments of human : metabolic diseases. |
format |
Text |
author |
Hapner, Hannah |
spellingShingle |
Hapner, Hannah Changing lanes : seasonal differences in energy metabolism of adipocytes in grizzly bears (Ursus arctos horribilis) |
author_facet |
Hapner, Hannah |
author_sort |
Hapner, Hannah |
title |
Changing lanes : seasonal differences in energy metabolism of adipocytes in grizzly bears (Ursus arctos horribilis) |
title_short |
Changing lanes : seasonal differences in energy metabolism of adipocytes in grizzly bears (Ursus arctos horribilis) |
title_full |
Changing lanes : seasonal differences in energy metabolism of adipocytes in grizzly bears (Ursus arctos horribilis) |
title_fullStr |
Changing lanes : seasonal differences in energy metabolism of adipocytes in grizzly bears (Ursus arctos horribilis) |
title_full_unstemmed |
Changing lanes : seasonal differences in energy metabolism of adipocytes in grizzly bears (Ursus arctos horribilis) |
title_sort |
changing lanes : seasonal differences in energy metabolism of adipocytes in grizzly bears (ursus arctos horribilis) |
publisher |
Washington State University |
publishDate |
2022 |
url |
https://dx.doi.org/10.7273/000000056 https://rex.libraries.wsu.edu/esploro/outputs/graduate/99900587063801842 |
long_lat |
ENVELOPE(18.933,18.933,69.617,69.617) |
geographic |
Lanes |
geographic_facet |
Lanes |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_rights |
Embargo |
op_doi |
https://doi.org/10.7273/000000056 |
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1766231546871676928 |