Systematic characterization of immunoglobulin loci and deep sequencing of the expressed repertoire in the Atlantic cod (Gadus morhua) ...

• Main Authors: Györkei, Ádám, Johansen, Finn-Eirik, Qiao, Shuo-Wang
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: figshare 2024
• Subjects: Genetics; FOS: Biological sciences; Immunology; FOS: Clinical medicine; atlantic cod; Gadus morhua
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.7314978.v1; https://springernature.figshare.com/collections/Systematic_characterization_of_immunoglobulin_loci_and_deep_sequencing_of_the_expressed_repertoire_in_the_Atlantic_cod_Gadus_morhua_/7314978/1

Abstract Background The Atlantic cod is a prolific species in the Atlantic, despite its inconsistent specific antibody response. It presents a peculiar case within vertebrate immunology due to its distinct immune system, characterized by the absence of MHCII antigen presentation pathway, required for T cell-dependent antibody responses. Thorough characterisation of immunoglobulin loci and analysis of the antibody repertoire is necessary to further our understanding of the Atlantic cod’s immune response on a molecular level. Results A comprehensive search of the cod genome (gadmor3.0) identified the complete set of IgH genes organized into three sequential translocons on chromosome 2, while IgL genes were located on chromosomes 2 and 5. The Atlantic cod displayed a moderate germline V gene diversity, comprising four V gene families for both IgH and IgL, each with distinct chromosomal locations and organizational structures. 5’RACE sequencing revealed a diverse range of heavy chain CDR3 sequences and ...