Supplementary material from "Tissue-specific metabolic enzyme levels covary with whole-animal metabolic rates and life-history loci via epistatic effects" ...

• Main Authors: Prokkola, Jenni M., Chew, Kuan Kiat, Anttila, Katja, Maamela, Katja S., Yildiz, Atakan, Åsheim, Eirik R., Primmer, Craig R., Aykanat, Tutku
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: The Royal Society 2023
• Subjects: Evolutionary biology not elsewhere classified; Animal physiology - biophysics; Animal physiology - cell; Animal physiology - systems; Atlantic salmon; Salmo salar
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.6981758.v1; https://rs.figshare.com/collections/Supplementary_material_from_Tissue-specific_metabolic_enzyme_levels_covary_with_whole-animal_metabolic_rates_and_life-history_loci_via_epistatic_effects_/6981758/1

Metabolic rates, including standard (SMR) and maximum (MMR) metabolic rate have often been linked with life-history strategies. Variation in context- and tissue-level metabolism underlying SMR and MMR may thus provide a physiological basis for life-history variation. This raises a hypothesis that tissue-specific metabolism covaries with whole-animal metabolic rates and is genetically linked to life history. In Atlantic salmon ( Salmo salar ), variation in two loci, vgll3 and six6 , affects life history via age-at-maturity as well as MMR. Here, using individuals with known SMR and MMR with different vgll3 and six6 genotype combinations, we measured proxies of mitochondrial density and anaerobic metabolism, i.e. maximal activities of the mitochondrial citrate synthase (CS) and lactate dehydrogenase (LDH) enzymes, in four tissues (heart, intestine, liver, white muscle) across low- and high-food regimes. We found enzymatic activities were related to metabolic rates, mainly SMR, in the intestine and heart. ...