Interplay between autotrophic and heterotrophic prokaryotic metabolism in the bathypelagic realm revealed by metatranscriptomic analyses ...

• Main Authors: Srivastava, Abhishek, De Corte, Daniele, Garcia, Juan A. L., Swan, Brandon K., Stepanauskas, Ramunas, Herndl, Gerhard J., Sintes, Eva
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: figshare 2024
• Subjects: Microbiology; FOS: Biological sciences; Labrador Sea; North Atlantic
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.6914741; https://springernature.figshare.com/collections/Interplay_between_autotrophic_and_heterotrophic_prokaryotic_metabolism_in_the_bathypelagic_realm_revealed_by_metatranscriptomic_analyses/6914741

Abstract Background Heterotrophic microbes inhabiting the dark ocean largely depend on the settling of organic matter from the sunlit ocean. However, this sinking of organic materials is insufficient to cover their demand for energy and alternative sources such as chemoautotrophy have been proposed. Reduced sulfur compounds, such as thiosulfate, are a potential energy source for both auto- and heterotrophic marine prokaryotes. Methods Seawater samples were collected from Labrador Sea Water (LSW, ~ 2000 m depth) in the North Atlantic and incubated in the dark at in situ temperature unamended, amended with 1 µM thiosulfate, or with 1 µM thiosulfate plus 10 µM glucose and 10 µM acetate (thiosulfate plus dissolved organic matter, DOM). Inorganic carbon fixation was measured in the different treatments and samples for metatranscriptomic analyses were collected after 1 h and 72 h of incubation. Results Amendment of LSW with thiosulfate and thiosulfate plus DOM enhanced prokaryotic inorganic carbon fixation. The ...