Integrated analysis of mRNAs and lncRNAs reveals candidate marker genes and potential hub lncRNAs associated with growth regulation of the Pacific Oyster, Crassostrea gigas ...

• Main Authors: Li, Yongjing, Yang, Ben, Shi, Chenyu, Tan, Ying, Ren, Liting, Mokrani, Ahmed, Li, Qi, Liu, Shikai
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: figshare 2024
• Subjects: Genetics; FOS: Biological sciences; Crassostrea gigas; Pacific oyster
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.6785329; https://springernature.figshare.com/collections/Integrated_analysis_of_mRNAs_and_lncRNAs_reveals_candidate_marker_genes_and_potential_hub_lncRNAs_associated_with_growth_regulation_of_the_Pacific_Oyster_Crassostrea_gigas/6785329

Abstract Background The Pacific oyster, Crassostrea gigas, is an economically important shellfish around the world. Great efforts have been made to improve its growth rate through genetic breeding. However, the candidate marker genes, pathways, and potential lncRNAs involved in oyster growth regulation remain largely unknown. To identify genes, lncRNAs, and pathways involved in growth regulation, C. gigas spat was cultured at a low temperature (15 ℃) to yield a growth-inhibited model, which was used to conduct comparative transcriptome analysis with spat cultured at normal temperature (25 ℃). Results In total, 8627 differentially expressed genes (DEGs) and 1072 differentially expressed lncRNAs (DELs) were identified between the normal-growth oysters (cultured at 25 ℃, hereinafter referred to as NG) and slow-growth oysters (cultured at 15 ℃, hereinafter referred to as SG). Functional enrichment analysis showed that these DEGs were mostly enriched in the AMPK signaling pathway, MAPK signaling pathway, insulin ...