Bacterial symbiont diversity in Arctic seep Oligobrachia siboglinids ...

• Main Authors: Sen, Arunima, Tanguy, Gwenn, Galand, Pierre E., Andersen, Ann C., Hourdez, Stéphane
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: figshare 2024
• Subjects: Microbiology; FOS: Biological sciences; Arctic
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.6676396; https://springernature.figshare.com/collections/Bacterial_symbiont_diversity_in_Arctic_seep_Oligobrachia_siboglinids/6676396

Abstract Background High latitude seeps are dominated by Oligobrachia siboglinid worms. Since these worms are often the sole chemosymbiotrophic taxon present (they host chemosynthetic bacteria within the trophosome organ in their trunk region), a key question in the study of high latitude seep ecology has been whether they harbor methanotrophic symbionts. This debate has manifested due to the mismatch between stable carbon isotope signatures of the worms (lower than -50‰ and usually indicative of methanotrophic symbioses) and the lack of molecular or microscopic evidence for methanotrophic symbionts. Two hypotheses have circulated to explain this paradox: (1) the uptake of sediment carbon compounds with depleted δC13 values from the seep environment, and (2) a small, but significant and difficult to detect population of methanotrophic symbionts. We conducted 16S rRNA amplicon sequencing of the V3-V4 regions on two species of northern seep Oligobrachia (Oligobrachia webbi and Oligobrachia sp. CPL-clade), from ...