Understanding host response to infectious salmon anaemia virus in an Atlantic salmon cell line using single-cell RNA sequencing ...
Abstract Background Infectious Salmon Anaemia Virus (ISAV) is an Orthomixovirus that represents a large problem for salmonid aquaculture worldwide. Current prevention and treatment methods are only partially effective. Genetic selection and genome engineering have the potential to develop ISAV resis...
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ftdatacite:10.6084/m9.figshare.c.6596311 2024-09-15T17:56:05+00:00 Understanding host response to infectious salmon anaemia virus in an Atlantic salmon cell line using single-cell RNA sequencing ... Gervais, Ophélie Peñaloza, Carolina Gratacap, Remi Papadopoulou, Athina Beltrán, Mariana Henderson, Neil C. Houston, Ross D. Hassan, Musa A. Robledo, Diego 2024 https://dx.doi.org/10.6084/m9.figshare.c.6596311 https://springernature.figshare.com/collections/Understanding_host_response_to_infectious_salmon_anaemia_virus_in_an_Atlantic_salmon_cell_line_using_single-cell_RNA_sequencing/6596311 unknown figshare Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 Genetics FOS: Biological sciences Collection article 2024 ftdatacite https://doi.org/10.6084/m9.figshare.c.6596311 2024-09-02T08:18:07Z Abstract Background Infectious Salmon Anaemia Virus (ISAV) is an Orthomixovirus that represents a large problem for salmonid aquaculture worldwide. Current prevention and treatment methods are only partially effective. Genetic selection and genome engineering have the potential to develop ISAV resistant salmon stocks. Both strategies can benefit from an improved understanding of the genomic regulation of ISAV pathogenesis. Here, we used single-cell RNA sequencing of an Atlantic salmon cell line to provide the first high dimensional insight into the transcriptional landscape that underpins host-virus interaction during early ISAV infection. Results Salmon head kidney (SHK-1) cells were single-cell RNA sequenced at 24, 48 and 96 h post-ISAV challenge. At 24 h post infection, cells showed expression signatures consistent with viral entry, with genes such as PI3K, FAK or JNK being upregulated relative to uninfected cells. At 48 and 96 h, infected cells showed a clear anti-viral response, characterised by the ... Article in Journal/Newspaper Atlantic salmon DataCite |
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Genetics FOS: Biological sciences |
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Genetics FOS: Biological sciences Gervais, Ophélie Peñaloza, Carolina Gratacap, Remi Papadopoulou, Athina Beltrán, Mariana Henderson, Neil C. Houston, Ross D. Hassan, Musa A. Robledo, Diego Understanding host response to infectious salmon anaemia virus in an Atlantic salmon cell line using single-cell RNA sequencing ... |
topic_facet |
Genetics FOS: Biological sciences |
description |
Abstract Background Infectious Salmon Anaemia Virus (ISAV) is an Orthomixovirus that represents a large problem for salmonid aquaculture worldwide. Current prevention and treatment methods are only partially effective. Genetic selection and genome engineering have the potential to develop ISAV resistant salmon stocks. Both strategies can benefit from an improved understanding of the genomic regulation of ISAV pathogenesis. Here, we used single-cell RNA sequencing of an Atlantic salmon cell line to provide the first high dimensional insight into the transcriptional landscape that underpins host-virus interaction during early ISAV infection. Results Salmon head kidney (SHK-1) cells were single-cell RNA sequenced at 24, 48 and 96 h post-ISAV challenge. At 24 h post infection, cells showed expression signatures consistent with viral entry, with genes such as PI3K, FAK or JNK being upregulated relative to uninfected cells. At 48 and 96 h, infected cells showed a clear anti-viral response, characterised by the ... |
format |
Article in Journal/Newspaper |
author |
Gervais, Ophélie Peñaloza, Carolina Gratacap, Remi Papadopoulou, Athina Beltrán, Mariana Henderson, Neil C. Houston, Ross D. Hassan, Musa A. Robledo, Diego |
author_facet |
Gervais, Ophélie Peñaloza, Carolina Gratacap, Remi Papadopoulou, Athina Beltrán, Mariana Henderson, Neil C. Houston, Ross D. Hassan, Musa A. Robledo, Diego |
author_sort |
Gervais, Ophélie |
title |
Understanding host response to infectious salmon anaemia virus in an Atlantic salmon cell line using single-cell RNA sequencing ... |
title_short |
Understanding host response to infectious salmon anaemia virus in an Atlantic salmon cell line using single-cell RNA sequencing ... |
title_full |
Understanding host response to infectious salmon anaemia virus in an Atlantic salmon cell line using single-cell RNA sequencing ... |
title_fullStr |
Understanding host response to infectious salmon anaemia virus in an Atlantic salmon cell line using single-cell RNA sequencing ... |
title_full_unstemmed |
Understanding host response to infectious salmon anaemia virus in an Atlantic salmon cell line using single-cell RNA sequencing ... |
title_sort |
understanding host response to infectious salmon anaemia virus in an atlantic salmon cell line using single-cell rna sequencing ... |
publisher |
figshare |
publishDate |
2024 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.6596311 https://springernature.figshare.com/collections/Understanding_host_response_to_infectious_salmon_anaemia_virus_in_an_Atlantic_salmon_cell_line_using_single-cell_RNA_sequencing/6596311 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.6084/m9.figshare.c.6596311 |
_version_ |
1810432296276197376 |