Understanding host response to infectious salmon anaemia virus in an Atlantic salmon cell line using single-cell RNA sequencing ...

• Main Authors: Gervais, Ophélie, Peñaloza, Carolina, Gratacap, Remi, Papadopoulou, Athina, Beltrán, Mariana, Henderson, Neil C., Houston, Ross D., Hassan, Musa A., Robledo, Diego
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: figshare 2024
• Subjects: Genetics; FOS: Biological sciences; Atlantic salmon
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.6596311.v1; https://springernature.figshare.com/collections/Understanding_host_response_to_infectious_salmon_anaemia_virus_in_an_Atlantic_salmon_cell_line_using_single-cell_RNA_sequencing/6596311/1

Abstract Background Infectious Salmon Anaemia Virus (ISAV) is an Orthomixovirus that represents a large problem for salmonid aquaculture worldwide. Current prevention and treatment methods are only partially effective. Genetic selection and genome engineering have the potential to develop ISAV resistant salmon stocks. Both strategies can benefit from an improved understanding of the genomic regulation of ISAV pathogenesis. Here, we used single-cell RNA sequencing of an Atlantic salmon cell line to provide the first high dimensional insight into the transcriptional landscape that underpins host-virus interaction during early ISAV infection. Results Salmon head kidney (SHK-1) cells were single-cell RNA sequenced at 24, 48 and 96 h post-ISAV challenge. At 24 h post infection, cells showed expression signatures consistent with viral entry, with genes such as PI3K, FAK or JNK being upregulated relative to uninfected cells. At 48 and 96 h, infected cells showed a clear anti-viral response, characterised by the ...