Heterologous production and characterization of a pyomelanin of Antarctic Pseudomonas sp. ANT_H4: a metabolite protecting against UV and free radicals, interacting with iron from minerals and exhibiting priming properties toward plant hairy roots ...

• Main Authors: Styczynski, Michal, Rogowska, Agata, Nyabayo, Christine, Decewicz, Przemyslaw, Romaniuk, Filip, Pączkowski, Cezary, Szakiel, Anna, Suessmuth, Roderich, Dziewit, Lukasz
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: figshare 2024
• Subjects: Microbiology; FOS: Biological sciences; Antarc*; Antarctic; Antarctica
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.6586255.v1; https://springernature.figshare.com/collections/Heterologous_production_and_characterization_of_a_pyomelanin_of_Antarctic_Pseudomonas_sp_ANT_H4_a_metabolite_protecting_against_UV_and_free_radicals_interacting_with_iron_from_minerals_and_exhibiting_priming_properties_toward_plant_hairy_ro/6586255/1

Abstract Background Antarctica has one of the most extreme environments in the world. This region is inhabited by specifically adapted microorganisms that produce various unique secondary metabolites (e.g. pigments) enabling their survival under the harsh environmental conditions. It was already shown that these natural, biologically active molecules may find application in various fields of biotechnology. Results In this study, a cold-active brown-pigment-producing Pseudomonas sp. ANT_H4 strain was characterized. In-depth genomic analysis combined with the application of a fosmid expression system revealed two different pathways of melanin-like compounds biosynthesis by the ANT_H4 strain. The chromatographic behavior and Fourier-transform infrared spectroscopic analyses allowed for the identification of the extracted melanin-like compound as a pyomelanin. Furthermore, optimization of the production and thorough functional analyses of the pyomelanin were performed to test its usability in biotechnology. It ...