Transcriptome analysis reveals the high temperature induced damage is a significant factor affecting the osmotic function of gill tissue in Siberian sturgeon (Acipenser baerii) ...

• Main Authors: Yang, Shiyong, Li, Datian, Feng, Langkun, Zhang, Chaoyang, Xi, Dandan, Liu, Hongli, Yan, Chaozhan, Xu, Zihan, Zhang, Yujie, Li, Yunkun, Yan, Taiming, He, Zhi, Wu, Jiayun, Gong, Quan, Du, Jun, Huang, Xiaoli, Du, Xiaogang
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: figshare 2023
• Subjects: Biochemistry; Cell Biology; Molecular Biology; Physiology; FOS Biological sciences; Environmental Sciences not elsewhere classified; Ecology; Biological Sciences not elsewhere classified; Inorganic Chemistry; FOS Chemical sciences; Acipenser baerii; Siberian sturgeon
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.6583009.v1; https://springernature.figshare.com/collections/Transcriptome_analysis_reveals_the_high_temperature_induced_damage_is_a_significant_factor_affecting_the_osmotic_function_of_gill_tissue_in_Siberian_sturgeon_Acipenser_baerii_/6583009/1

Abstract Background Maintaining osmotic equilibrium plays an important role in the survival of cold-water fishes. Heat stress has been proven to reduce the activity of Na+/K+-ATPase in the gill tissue, leading to destruction of the osmotic equilibrium. However, the mechanism of megatemperature affecting gill osmoregulation has not been fully elucidated. Results In this study, Siberian sturgeon (Acipenser baerii) was used to analyze histopathological change, plasma ion level, and transcriptome of gill tissue subjected to 20℃, 24℃and 28℃. The results showed that ROS level and damage were increased in gill tissue with the increasing of heat stress temperature. Plasma Cl− level at 28℃ was distinctly lower than that at 20℃ and 24℃, while no significant difference was found in Na+ and K+ ion levels among different groups. Transcriptome analysis displayed that osmoregulation-, DNA-repair- and apoptosis-related terms or pathways were enriched in GO and KEGG analysis. Moreover, 194 osmoregulation-related genes were ...