The methylome of Biomphalaria glabrata and other mollusks: enduring modification of epigenetic landscape and phenotypic traits by a new DNA methylation inhibitor
Abstract Background 5-Methylcytosine (5mC) is an important epigenetic mark in eukaryotes. Little information about its role exists for invertebrates. To investigate the contribution of 5mC to phenotypic variation in invertebrates, alteration of methylation patterns needs to be produced. Here, we app...
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ftdatacite:10.6084/m9.figshare.c.5679794.v1 2023-05-15T15:58:51+02:00 The methylome of Biomphalaria glabrata and other mollusks: enduring modification of epigenetic landscape and phenotypic traits by a new DNA methylation inhibitor Luviano, Nelia Lopez, Marie Gawehns, Fleur Chaparro, Cristian Arimondo, Paola B. Ivanovic, Slavica David, Patrice Verhoeven, Koen Cosseau, Céline Grunau, Christoph 2021 https://dx.doi.org/10.6084/m9.figshare.c.5679794.v1 https://springernature.figshare.com/collections/The_methylome_of_Biomphalaria_glabrata_and_other_mollusks_enduring_modification_of_epigenetic_landscape_and_phenotypic_traits_by_a_new_DNA_methylation_inhibitor/5679794/1 unknown figshare https://dx.doi.org/10.1186/s13072-021-00422-7 https://dx.doi.org/10.6084/m9.figshare.c.5679794 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Genetics FOS Biological sciences Collection article 2021 ftdatacite https://doi.org/10.6084/m9.figshare.c.5679794.v1 https://doi.org/10.1186/s13072-021-00422-7 https://doi.org/10.6084/m9.figshare.c.5679794 2022-02-08T12:05:56Z Abstract Background 5-Methylcytosine (5mC) is an important epigenetic mark in eukaryotes. Little information about its role exists for invertebrates. To investigate the contribution of 5mC to phenotypic variation in invertebrates, alteration of methylation patterns needs to be produced. Here, we apply new non-nucleoside DNA methyltransferase inhibitors (DNMTi) to introduce aleatory changes into the methylome of mollusk species. Results Flavanone inhibitor Flv1 was efficient in reducing 5mC in the freshwater snails Biomphalaria glabrata and Physa acuta, and to a lesser degree, probably due to lower stability in sea water, in the oyster Crassostrea gigas. Flv1 has no toxic effects and significantly decreased the 5mC level in the treated B. glabrata and in its offspring. Drug treatment triggers significant variation in the shell height in both generations. A reduced representation bisulfite-sequencing method called epiGBS corroborates hypomethylation effect of Flv1 in both B. glabrata generations and identifies seven Differential Methylated Regions (DMR) out of 32 found both in Flv1-exposed snails and its progeny, from which 5 were hypomethylated, demonstrating a multigenerational effect. By targeted bisulfite sequencing, we confirmed hypomethylation in a locus and show that it is associated with reduced gene expression. Conclusions Flv1 is a new and efficient DNMTi that can be used to induce transient and heritable modifications of the epigenetic landscape and phenotypic traits in mollusks, a phylum of the invertebrates in which epigenetics is understudied. Article in Journal/Newspaper Crassostrea gigas DataCite Metadata Store (German National Library of Science and Technology) |
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DataCite Metadata Store (German National Library of Science and Technology) |
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unknown |
topic |
Genetics FOS Biological sciences |
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Genetics FOS Biological sciences Luviano, Nelia Lopez, Marie Gawehns, Fleur Chaparro, Cristian Arimondo, Paola B. Ivanovic, Slavica David, Patrice Verhoeven, Koen Cosseau, Céline Grunau, Christoph The methylome of Biomphalaria glabrata and other mollusks: enduring modification of epigenetic landscape and phenotypic traits by a new DNA methylation inhibitor |
topic_facet |
Genetics FOS Biological sciences |
description |
Abstract Background 5-Methylcytosine (5mC) is an important epigenetic mark in eukaryotes. Little information about its role exists for invertebrates. To investigate the contribution of 5mC to phenotypic variation in invertebrates, alteration of methylation patterns needs to be produced. Here, we apply new non-nucleoside DNA methyltransferase inhibitors (DNMTi) to introduce aleatory changes into the methylome of mollusk species. Results Flavanone inhibitor Flv1 was efficient in reducing 5mC in the freshwater snails Biomphalaria glabrata and Physa acuta, and to a lesser degree, probably due to lower stability in sea water, in the oyster Crassostrea gigas. Flv1 has no toxic effects and significantly decreased the 5mC level in the treated B. glabrata and in its offspring. Drug treatment triggers significant variation in the shell height in both generations. A reduced representation bisulfite-sequencing method called epiGBS corroborates hypomethylation effect of Flv1 in both B. glabrata generations and identifies seven Differential Methylated Regions (DMR) out of 32 found both in Flv1-exposed snails and its progeny, from which 5 were hypomethylated, demonstrating a multigenerational effect. By targeted bisulfite sequencing, we confirmed hypomethylation in a locus and show that it is associated with reduced gene expression. Conclusions Flv1 is a new and efficient DNMTi that can be used to induce transient and heritable modifications of the epigenetic landscape and phenotypic traits in mollusks, a phylum of the invertebrates in which epigenetics is understudied. |
format |
Article in Journal/Newspaper |
author |
Luviano, Nelia Lopez, Marie Gawehns, Fleur Chaparro, Cristian Arimondo, Paola B. Ivanovic, Slavica David, Patrice Verhoeven, Koen Cosseau, Céline Grunau, Christoph |
author_facet |
Luviano, Nelia Lopez, Marie Gawehns, Fleur Chaparro, Cristian Arimondo, Paola B. Ivanovic, Slavica David, Patrice Verhoeven, Koen Cosseau, Céline Grunau, Christoph |
author_sort |
Luviano, Nelia |
title |
The methylome of Biomphalaria glabrata and other mollusks: enduring modification of epigenetic landscape and phenotypic traits by a new DNA methylation inhibitor |
title_short |
The methylome of Biomphalaria glabrata and other mollusks: enduring modification of epigenetic landscape and phenotypic traits by a new DNA methylation inhibitor |
title_full |
The methylome of Biomphalaria glabrata and other mollusks: enduring modification of epigenetic landscape and phenotypic traits by a new DNA methylation inhibitor |
title_fullStr |
The methylome of Biomphalaria glabrata and other mollusks: enduring modification of epigenetic landscape and phenotypic traits by a new DNA methylation inhibitor |
title_full_unstemmed |
The methylome of Biomphalaria glabrata and other mollusks: enduring modification of epigenetic landscape and phenotypic traits by a new DNA methylation inhibitor |
title_sort |
methylome of biomphalaria glabrata and other mollusks: enduring modification of epigenetic landscape and phenotypic traits by a new dna methylation inhibitor |
publisher |
figshare |
publishDate |
2021 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.5679794.v1 https://springernature.figshare.com/collections/The_methylome_of_Biomphalaria_glabrata_and_other_mollusks_enduring_modification_of_epigenetic_landscape_and_phenotypic_traits_by_a_new_DNA_methylation_inhibitor/5679794/1 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
https://dx.doi.org/10.1186/s13072-021-00422-7 https://dx.doi.org/10.6084/m9.figshare.c.5679794 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.5679794.v1 https://doi.org/10.1186/s13072-021-00422-7 https://doi.org/10.6084/m9.figshare.c.5679794 |
_version_ |
1766394627002204160 |