Supplementary material from "Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome"
The domestic dog has inhabited the anthropogenic niche for at least 15 000 years, but despite their impact on human strategies, the lives of dogs and their interactions with humans have only recently become a subject of interest to archaeologists. In the Arctic, dogs rely exclusively on humans for f...
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2021
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ftdatacite:10.6084/m9.figshare.c.5470468.v1 2023-05-15T15:07:00+02:00 Supplementary material from "Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome" Runge, Anne Kathrine W. Hendy, Jessica Richter, Kristine K. Masson-MacLean, Edouard Britton, Kate Mackie, Meaghan McGrath, Krista Collins, Matthew Cappellini, Enrico Speller, Camilla 2021 https://dx.doi.org/10.6084/m9.figshare.c.5470468.v1 https://rs.figshare.com/collections/Supplementary_material_from_Palaeoproteomic_analyses_of_dog_palaeofaeces_reveal_a_preserved_dietary_and_host_digestive_proteome_/5470468/1 unknown The Royal Society https://dx.doi.org/10.1098/rspb.2021.0020 https://dx.doi.org/10.6084/m9.figshare.c.5470468 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Biochemistry Evolutionary Biology FOS Biological sciences 60801 Animal Behaviour Collection article 2021 ftdatacite https://doi.org/10.6084/m9.figshare.c.5470468.v1 https://doi.org/10.1098/rspb.2021.0020 https://doi.org/10.6084/m9.figshare.c.5470468 2021-11-05T12:55:41Z The domestic dog has inhabited the anthropogenic niche for at least 15 000 years, but despite their impact on human strategies, the lives of dogs and their interactions with humans have only recently become a subject of interest to archaeologists. In the Arctic, dogs rely exclusively on humans for food during the winter, and while stable isotope analyses have revealed dietary similarities at some sites, deciphering the details of provisioning strategies have been challenging. In this study, we apply Zooarchaeology by Mass Spectrometry (ZooMS) and liquid chromatography tandem mass spectrometry (LC-MS/MS) to dog palaeofaeces to investigate protein preservation in this highly degradable material and obtain information about the diet of domestic dogs at the Nunalleq site, Alaska. We identify a suite of digestive and metabolic proteins from the host species, demonstrating the utility of this material as a novel and viable substrate for the recovery of gastrointestinal proteomes. The recovered proteins revealed that the Nunalleq dogs consumed a range of Pacific salmon species (coho, chum, chinook and sockeye) and that the consumed tissues derived from muscle and bone tissues as well as roe and guts. Overall, the study demonstrated the viability of permafrost-preserved palaeofaeces as a unique source of host and dietary proteomes. Article in Journal/Newspaper Arctic permafrost Alaska DataCite Metadata Store (German National Library of Science and Technology) Arctic Pacific Sockeye ENVELOPE(-130.143,-130.143,54.160,54.160) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Biochemistry Evolutionary Biology FOS Biological sciences 60801 Animal Behaviour |
spellingShingle |
Biochemistry Evolutionary Biology FOS Biological sciences 60801 Animal Behaviour Runge, Anne Kathrine W. Hendy, Jessica Richter, Kristine K. Masson-MacLean, Edouard Britton, Kate Mackie, Meaghan McGrath, Krista Collins, Matthew Cappellini, Enrico Speller, Camilla Supplementary material from "Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome" |
topic_facet |
Biochemistry Evolutionary Biology FOS Biological sciences 60801 Animal Behaviour |
description |
The domestic dog has inhabited the anthropogenic niche for at least 15 000 years, but despite their impact on human strategies, the lives of dogs and their interactions with humans have only recently become a subject of interest to archaeologists. In the Arctic, dogs rely exclusively on humans for food during the winter, and while stable isotope analyses have revealed dietary similarities at some sites, deciphering the details of provisioning strategies have been challenging. In this study, we apply Zooarchaeology by Mass Spectrometry (ZooMS) and liquid chromatography tandem mass spectrometry (LC-MS/MS) to dog palaeofaeces to investigate protein preservation in this highly degradable material and obtain information about the diet of domestic dogs at the Nunalleq site, Alaska. We identify a suite of digestive and metabolic proteins from the host species, demonstrating the utility of this material as a novel and viable substrate for the recovery of gastrointestinal proteomes. The recovered proteins revealed that the Nunalleq dogs consumed a range of Pacific salmon species (coho, chum, chinook and sockeye) and that the consumed tissues derived from muscle and bone tissues as well as roe and guts. Overall, the study demonstrated the viability of permafrost-preserved palaeofaeces as a unique source of host and dietary proteomes. |
format |
Article in Journal/Newspaper |
author |
Runge, Anne Kathrine W. Hendy, Jessica Richter, Kristine K. Masson-MacLean, Edouard Britton, Kate Mackie, Meaghan McGrath, Krista Collins, Matthew Cappellini, Enrico Speller, Camilla |
author_facet |
Runge, Anne Kathrine W. Hendy, Jessica Richter, Kristine K. Masson-MacLean, Edouard Britton, Kate Mackie, Meaghan McGrath, Krista Collins, Matthew Cappellini, Enrico Speller, Camilla |
author_sort |
Runge, Anne Kathrine W. |
title |
Supplementary material from "Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome" |
title_short |
Supplementary material from "Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome" |
title_full |
Supplementary material from "Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome" |
title_fullStr |
Supplementary material from "Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome" |
title_full_unstemmed |
Supplementary material from "Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome" |
title_sort |
supplementary material from "palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome" |
publisher |
The Royal Society |
publishDate |
2021 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.5470468.v1 https://rs.figshare.com/collections/Supplementary_material_from_Palaeoproteomic_analyses_of_dog_palaeofaeces_reveal_a_preserved_dietary_and_host_digestive_proteome_/5470468/1 |
long_lat |
ENVELOPE(-130.143,-130.143,54.160,54.160) |
geographic |
Arctic Pacific Sockeye |
geographic_facet |
Arctic Pacific Sockeye |
genre |
Arctic permafrost Alaska |
genre_facet |
Arctic permafrost Alaska |
op_relation |
https://dx.doi.org/10.1098/rspb.2021.0020 https://dx.doi.org/10.6084/m9.figshare.c.5470468 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.5470468.v1 https://doi.org/10.1098/rspb.2021.0020 https://doi.org/10.6084/m9.figshare.c.5470468 |
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1766338577339252736 |