Molecular genetic diversity and population structure analyses of rutabaga accessions from Nordic countries as revealed by single nucleotide polymorphism markers

• Main Authors: Yu, Zhiyu, Fredua-Agyeman, Rudolph, Hwang, Sheau-Fang, Strelkov, Stephen E.
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: figshare 2021
• Subjects: Genetics; FOS Biological sciences; Norway; Iceland
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.5465218; https://springernature.figshare.com/collections/Molecular_genetic_diversity_and_population_structure_analyses_of_rutabaga_accessions_from_Nordic_countries_as_revealed_by_single_nucleotide_polymorphism_markers/5465218

Abstract Background Rutabaga or swede (Brassica napus ssp. napobrassica (L.) Hanelt) varies in root and leaf shape and colour, flesh colour, foliage growth habits, maturity date, seed quality parameters, disease resistance and other traits. Despite these morphological differences, no in-depth molecular analyses of genetic diversity have been conducted in this crop. Understanding this diversity is important for conservation and broadening the use of this resource. Results This study investigated the genetic diversity within and among 124 rutabaga accessions from five Nordic countries (Norway, Sweden, Finland, Denmark and Iceland) using a 15 K single nucleotide polymorphism (SNP) Brassica array. After excluding markers that did not amplify genomic DNA, monomorphic and low coverage site markers, the accessions were analyzedwith 6861 SNP markers. Allelic frequency statistics, including polymorphism information content (PIC), minor allele frequency (MAF) and mean expected heterozygosity ( H ¯ $$ \overline{H} $$ e) and population differentiation statistics such as Wright’s F-statistics (FST) and analysis of molecular variance (AMOVA) indicated that the rutabaga accessions from Norway, Sweden, Finland and Denmark were not genetically different from each other. In contrast, accessions from these countries were significantly different from the accessions from Iceland (P