Comparison of mitochondrial genome and development of specific PCR primers for identifying two scuticociliates, Pseudocohnilembus persalinus and Uronema marinum ...

• Main Authors: Huang, Yu-Xi, Wang, Sen, Gao, Yan-Qi, Chen, Jie-Hu, Wang, Xiu-Li, Li, Rui-Jun
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: figshare 2021
• Subjects: Genetics; FOS Biological sciences; Scophthalmus maximus
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.5462748; https://springernature.figshare.com/collections/Comparison_of_mitochondrial_genome_and_development_of_specific_PCR_primers_for_identifying_two_scuticociliates_Pseudocohnilembus_persalinus_and_Uronema_marinum/5462748

Abstract Background Pseudocohnilembus persalinus and Uronema marinum (Ciliophora, Scuticociliatia), as parasitic scuticociliatid ciliates, were isolated from Scophthalmus maximus and Takifugu rubripes, respectively, in our previous studies. These ciliates are morphologically very similar; hence, it is difficult to identify specific scuticociliate species using traditional classification methods for performing taxonomic research and disease control studies. Methods We annotated the mitochondrial genomes of these two scuticociliates on the basis of previous sequencing, including analyses of nucleotide composition, codon usage, Ka/Ks, and p-distance. We also compared the nucleotide and amino acid similarity of the mitochondrial genomes of P. persalinus, U. marinum, and other 12 related ciliates, and a phylogenetic tree was constructed using 16 common genes. We chose the nad4 and nad7 genes to design specific PCR primers for identification. Results P. persalinus and U. marinum were found to have a close ...