Genomic reconstruction of fossil and living microorganisms in ancient Siberian permafrost ...
Abstract Background Total DNA (intracellular, iDNA and extracellular, eDNA) from ancient permafrost records the mixed genetic repository of the past and present microbial populations through geological time. Given the exceptional preservation of eDNA under perennial frozen conditions, typical metage...
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ftdatacite:10.6084/m9.figshare.c.5427412.v1 2024-04-28T08:35:39+00:00 Genomic reconstruction of fossil and living microorganisms in ancient Siberian permafrost ... Liang, Renxing Li, Zhou Lau Vetter, Maggie C. Y. Vishnivetskaya, Tatiana A. Zanina, Oksana G. Lloyd, Karen G. Pfiffner, Susan M. Rivkina, Elizaveta M. Wang, Wei Wiggins, Jessica Miller, Jennifer Hettich, Robert L. Onstott, Tullis C. 2021 https://dx.doi.org/10.6084/m9.figshare.c.5427412.v1 https://springernature.figshare.com/collections/Genomic_reconstruction_of_fossil_and_living_microorganisms_in_ancient_Siberian_permafrost/5427412/1 unknown figshare https://dx.doi.org/10.6084/m9.figshare.c.5427412 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 Microbiology FOS Biological sciences Genetics Geology FOS Earth and related environmental sciences Collection article 2021 ftdatacite https://doi.org/10.6084/m9.figshare.c.5427412.v110.6084/m9.figshare.c.5427412 2024-04-02T12:02:45Z Abstract Background Total DNA (intracellular, iDNA and extracellular, eDNA) from ancient permafrost records the mixed genetic repository of the past and present microbial populations through geological time. Given the exceptional preservation of eDNA under perennial frozen conditions, typical metagenomic sequencing of total DNA precludes the discrimination between fossil and living microorganisms in ancient cryogenic environments. DNA repair protocols were combined with high throughput sequencing (HTS) of separate iDNA and eDNA fraction to reconstruct metagenome-assembled genomes (MAGs) from ancient microbial DNA entrapped in Siberian coastal permafrost. Results Despite the severe DNA damage in ancient permafrost, the coupling of DNA repair and HTS resulted in a total of 52 MAGs from sediments across a chronosequence (26–120 kyr). These MAGs were compared with those derived from the same samples but without utilizing DNA repair protocols. The MAGs from the youngest stratum showed minimal DNA damage and thus ... Article in Journal/Newspaper permafrost DataCite Metadata Store (German National Library of Science and Technology) |
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DataCite Metadata Store (German National Library of Science and Technology) |
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Microbiology FOS Biological sciences Genetics Geology FOS Earth and related environmental sciences |
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Microbiology FOS Biological sciences Genetics Geology FOS Earth and related environmental sciences Liang, Renxing Li, Zhou Lau Vetter, Maggie C. Y. Vishnivetskaya, Tatiana A. Zanina, Oksana G. Lloyd, Karen G. Pfiffner, Susan M. Rivkina, Elizaveta M. Wang, Wei Wiggins, Jessica Miller, Jennifer Hettich, Robert L. Onstott, Tullis C. Genomic reconstruction of fossil and living microorganisms in ancient Siberian permafrost ... |
topic_facet |
Microbiology FOS Biological sciences Genetics Geology FOS Earth and related environmental sciences |
description |
Abstract Background Total DNA (intracellular, iDNA and extracellular, eDNA) from ancient permafrost records the mixed genetic repository of the past and present microbial populations through geological time. Given the exceptional preservation of eDNA under perennial frozen conditions, typical metagenomic sequencing of total DNA precludes the discrimination between fossil and living microorganisms in ancient cryogenic environments. DNA repair protocols were combined with high throughput sequencing (HTS) of separate iDNA and eDNA fraction to reconstruct metagenome-assembled genomes (MAGs) from ancient microbial DNA entrapped in Siberian coastal permafrost. Results Despite the severe DNA damage in ancient permafrost, the coupling of DNA repair and HTS resulted in a total of 52 MAGs from sediments across a chronosequence (26–120 kyr). These MAGs were compared with those derived from the same samples but without utilizing DNA repair protocols. The MAGs from the youngest stratum showed minimal DNA damage and thus ... |
format |
Article in Journal/Newspaper |
author |
Liang, Renxing Li, Zhou Lau Vetter, Maggie C. Y. Vishnivetskaya, Tatiana A. Zanina, Oksana G. Lloyd, Karen G. Pfiffner, Susan M. Rivkina, Elizaveta M. Wang, Wei Wiggins, Jessica Miller, Jennifer Hettich, Robert L. Onstott, Tullis C. |
author_facet |
Liang, Renxing Li, Zhou Lau Vetter, Maggie C. Y. Vishnivetskaya, Tatiana A. Zanina, Oksana G. Lloyd, Karen G. Pfiffner, Susan M. Rivkina, Elizaveta M. Wang, Wei Wiggins, Jessica Miller, Jennifer Hettich, Robert L. Onstott, Tullis C. |
author_sort |
Liang, Renxing |
title |
Genomic reconstruction of fossil and living microorganisms in ancient Siberian permafrost ... |
title_short |
Genomic reconstruction of fossil and living microorganisms in ancient Siberian permafrost ... |
title_full |
Genomic reconstruction of fossil and living microorganisms in ancient Siberian permafrost ... |
title_fullStr |
Genomic reconstruction of fossil and living microorganisms in ancient Siberian permafrost ... |
title_full_unstemmed |
Genomic reconstruction of fossil and living microorganisms in ancient Siberian permafrost ... |
title_sort |
genomic reconstruction of fossil and living microorganisms in ancient siberian permafrost ... |
publisher |
figshare |
publishDate |
2021 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.5427412.v1 https://springernature.figshare.com/collections/Genomic_reconstruction_of_fossil_and_living_microorganisms_in_ancient_Siberian_permafrost/5427412/1 |
genre |
permafrost |
genre_facet |
permafrost |
op_relation |
https://dx.doi.org/10.6084/m9.figshare.c.5427412 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.6084/m9.figshare.c.5427412.v110.6084/m9.figshare.c.5427412 |
_version_ |
1797567678778441728 |