Metagenomic analysis reveals the shared and distinct features of the soil resistome across tundra, temperate prairie, and tropical ecosystems ...
Abstract Background Soil is an important reservoir of antibiotic resistance genes (ARGs), but their potential risk in different ecosystems as well as response to anthropogenic land use change is unknown. We used a metagenomic approach and datasets with well-characterized metadata to investigate ARG...
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ftdatacite:10.6084/m9.figshare.c.5425416 2024-04-28T08:40:51+00:00 Metagenomic analysis reveals the shared and distinct features of the soil resistome across tundra, temperate prairie, and tropical ecosystems ... Qian, Xun Gunturu, Santosh Guo, Jiarong Chai, Benli Cole, James R. Gu, Jie Tiedje, James M. 2021 https://dx.doi.org/10.6084/m9.figshare.c.5425416 https://springernature.figshare.com/collections/Metagenomic_analysis_reveals_the_shared_and_distinct_features_of_the_soil_resistome_across_tundra_temperate_prairie_and_tropical_ecosystems/5425416 unknown figshare Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 Microbiology FOS Biological sciences Environmental Sciences not elsewhere classified Chemical Sciences not elsewhere classified Ecology Biological Sciences not elsewhere classified Collection article 2021 ftdatacite https://doi.org/10.6084/m9.figshare.c.5425416 2024-04-02T12:02:45Z Abstract Background Soil is an important reservoir of antibiotic resistance genes (ARGs), but their potential risk in different ecosystems as well as response to anthropogenic land use change is unknown. We used a metagenomic approach and datasets with well-characterized metadata to investigate ARG types and amounts in soil DNA of three native ecosystems: Alaskan tundra, US Midwestern prairie, and Amazon rainforest, as well as the effect of conversion of the latter two to agriculture and pasture, respectively. Results High diversity (242 ARG subtypes) and abundance (0.184–0.242 ARG copies per 16S rRNA gene copy) were observed irrespective of ecosystem, with multidrug resistance and efflux pump the dominant class and mechanism. Ten regulatory genes were identified and they accounted for 13–35% of resistome abundances in soils, among them arlR, cpxR, ompR, vanR, and vanS were dominant and observed in all studied soils. We identified 55 non-regulatory ARGs shared by all 26 soil metagenomes of the three ... Article in Journal/Newspaper Tundra DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
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DataCite Metadata Store (German National Library of Science and Technology) |
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unknown |
topic |
Microbiology FOS Biological sciences Environmental Sciences not elsewhere classified Chemical Sciences not elsewhere classified Ecology Biological Sciences not elsewhere classified |
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Microbiology FOS Biological sciences Environmental Sciences not elsewhere classified Chemical Sciences not elsewhere classified Ecology Biological Sciences not elsewhere classified Qian, Xun Gunturu, Santosh Guo, Jiarong Chai, Benli Cole, James R. Gu, Jie Tiedje, James M. Metagenomic analysis reveals the shared and distinct features of the soil resistome across tundra, temperate prairie, and tropical ecosystems ... |
topic_facet |
Microbiology FOS Biological sciences Environmental Sciences not elsewhere classified Chemical Sciences not elsewhere classified Ecology Biological Sciences not elsewhere classified |
description |
Abstract Background Soil is an important reservoir of antibiotic resistance genes (ARGs), but their potential risk in different ecosystems as well as response to anthropogenic land use change is unknown. We used a metagenomic approach and datasets with well-characterized metadata to investigate ARG types and amounts in soil DNA of three native ecosystems: Alaskan tundra, US Midwestern prairie, and Amazon rainforest, as well as the effect of conversion of the latter two to agriculture and pasture, respectively. Results High diversity (242 ARG subtypes) and abundance (0.184–0.242 ARG copies per 16S rRNA gene copy) were observed irrespective of ecosystem, with multidrug resistance and efflux pump the dominant class and mechanism. Ten regulatory genes were identified and they accounted for 13–35% of resistome abundances in soils, among them arlR, cpxR, ompR, vanR, and vanS were dominant and observed in all studied soils. We identified 55 non-regulatory ARGs shared by all 26 soil metagenomes of the three ... |
format |
Article in Journal/Newspaper |
author |
Qian, Xun Gunturu, Santosh Guo, Jiarong Chai, Benli Cole, James R. Gu, Jie Tiedje, James M. |
author_facet |
Qian, Xun Gunturu, Santosh Guo, Jiarong Chai, Benli Cole, James R. Gu, Jie Tiedje, James M. |
author_sort |
Qian, Xun |
title |
Metagenomic analysis reveals the shared and distinct features of the soil resistome across tundra, temperate prairie, and tropical ecosystems ... |
title_short |
Metagenomic analysis reveals the shared and distinct features of the soil resistome across tundra, temperate prairie, and tropical ecosystems ... |
title_full |
Metagenomic analysis reveals the shared and distinct features of the soil resistome across tundra, temperate prairie, and tropical ecosystems ... |
title_fullStr |
Metagenomic analysis reveals the shared and distinct features of the soil resistome across tundra, temperate prairie, and tropical ecosystems ... |
title_full_unstemmed |
Metagenomic analysis reveals the shared and distinct features of the soil resistome across tundra, temperate prairie, and tropical ecosystems ... |
title_sort |
metagenomic analysis reveals the shared and distinct features of the soil resistome across tundra, temperate prairie, and tropical ecosystems ... |
publisher |
figshare |
publishDate |
2021 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.5425416 https://springernature.figshare.com/collections/Metagenomic_analysis_reveals_the_shared_and_distinct_features_of_the_soil_resistome_across_tundra_temperate_prairie_and_tropical_ecosystems/5425416 |
genre |
Tundra |
genre_facet |
Tundra |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.6084/m9.figshare.c.5425416 |
_version_ |
1797571346774884352 |