High prevalence, intensity, and genetic diversity of Trichinella spp. in wolverine (Gulo gulo) from Yukon, Canada
Abstract Background Species of Trichinella are globally important foodborne parasites infecting a number of domestic and wild vertebrates, including humans. Free-ranging carnivores can act as sentinel species for detection of Trichinella spp. Knowledge of the epidemiology of these parasites may help...
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ftdatacite:10.6084/m9.figshare.c.5331812 2023-05-15T16:32:19+02:00 High prevalence, intensity, and genetic diversity of Trichinella spp. in wolverine (Gulo gulo) from Yukon, Canada Sharma, Rajnish Harms, N. Jane Kukka, Piia M. Jung, Thomas S. Parker, Sarah E. Ross, Sasha Thompson, Peter Rosenthal, Benjamin Hoberg, Eric P. Jenkins, Emily J. 2021 https://dx.doi.org/10.6084/m9.figshare.c.5331812 https://springernature.figshare.com/collections/High_prevalence_intensity_and_genetic_diversity_of_Trichinella_spp_in_wolverine_Gulo_gulo_from_Yukon_Canada/5331812 unknown figshare https://dx.doi.org/10.1186/s13071-021-04636-2 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Medicine 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology FOS Biological sciences 69999 Biological Sciences not elsewhere classified 60506 Virology Computational Biology Collection article 2021 ftdatacite https://doi.org/10.6084/m9.figshare.c.5331812 https://doi.org/10.1186/s13071-021-04636-2 2022-02-08T12:31:06Z Abstract Background Species of Trichinella are globally important foodborne parasites infecting a number of domestic and wild vertebrates, including humans. Free-ranging carnivores can act as sentinel species for detection of Trichinella spp. Knowledge of the epidemiology of these parasites may help prevent Trichinella spp. infections in northern Canadian animals and people. Previous research on Trichinella spp. in wildlife from Yukon did not identify risk factors associated with infection, or the diversity and identity of species of Trichinella in regional circulation, based on geographically extensive sampling with large sample sizes. Methods In a cross-sectional study, we determined the prevalence, infection intensity, risk factors, and species or genotypes of Trichinella in wolverine (Gulo gulo) in two regions of Yukon, Canada, from 2013–2017. A double separatory funnel digestion method followed by mutiplex PCR and PCR-RFLP were used to recover and identify species of Trichinella, respectively. Results We found larvae of Trichinella in the tongues of 78% (95% CI 73–82) of 338 wolverine sampled. The odds of adult (≥ 2 years) and yearling (1 year) wolverine being Trichinella spp.-positive were four and two times higher, respectively, compared to juveniles (<1 year). The odds of Trichinella spp. presence were three times higher in wolverine from southeast than northwest Yukon. The mean intensity of infection was 22.6 ± 39 (SD, range 0.1–295) larvae per gram. Trichinella T6 was the predominant genotype (76%), followed by T. nativa (8%); mixed infections with Trichinella T6 and T. nativa (12%) were observed. In addition, T. spiralis was detected in one wolverine. Out of 22 isolates initially identified as T. nativa in multiplex PCR, 14 were analyzed by PCR-RFLP to distinguish them from T. chanchalensis, a recently discovered cryptic species, which cannot be distinguished from the T. nativa on multiplex PCR. Ten isolates were identified either as T. chanchalensis alone (n = 7), or mixed infection with T. chanchalensis and T. nativa (n = 2) or T. chanchalensis and Trichinella T6 (n = 1)]. Conclusions Wolverine hosted high prevalence, high larval intensity, and multiple species of Trichinella, likely due to their scavenging habits, apex position in the food chain, and wide home range. Wolverine (especially adult males) should be considered as a sentinel species for surveys for Trichinella spp. across their distributional range. Graphical Abstract Article in Journal/Newspaper Gulo gulo Yukon DataCite Metadata Store (German National Library of Science and Technology) Yukon Canada |
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DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
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language |
unknown |
topic |
Medicine 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology FOS Biological sciences 69999 Biological Sciences not elsewhere classified 60506 Virology Computational Biology |
spellingShingle |
Medicine 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology FOS Biological sciences 69999 Biological Sciences not elsewhere classified 60506 Virology Computational Biology Sharma, Rajnish Harms, N. Jane Kukka, Piia M. Jung, Thomas S. Parker, Sarah E. Ross, Sasha Thompson, Peter Rosenthal, Benjamin Hoberg, Eric P. Jenkins, Emily J. High prevalence, intensity, and genetic diversity of Trichinella spp. in wolverine (Gulo gulo) from Yukon, Canada |
topic_facet |
Medicine 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology FOS Biological sciences 69999 Biological Sciences not elsewhere classified 60506 Virology Computational Biology |
description |
Abstract Background Species of Trichinella are globally important foodborne parasites infecting a number of domestic and wild vertebrates, including humans. Free-ranging carnivores can act as sentinel species for detection of Trichinella spp. Knowledge of the epidemiology of these parasites may help prevent Trichinella spp. infections in northern Canadian animals and people. Previous research on Trichinella spp. in wildlife from Yukon did not identify risk factors associated with infection, or the diversity and identity of species of Trichinella in regional circulation, based on geographically extensive sampling with large sample sizes. Methods In a cross-sectional study, we determined the prevalence, infection intensity, risk factors, and species or genotypes of Trichinella in wolverine (Gulo gulo) in two regions of Yukon, Canada, from 2013–2017. A double separatory funnel digestion method followed by mutiplex PCR and PCR-RFLP were used to recover and identify species of Trichinella, respectively. Results We found larvae of Trichinella in the tongues of 78% (95% CI 73–82) of 338 wolverine sampled. The odds of adult (≥ 2 years) and yearling (1 year) wolverine being Trichinella spp.-positive were four and two times higher, respectively, compared to juveniles (<1 year). The odds of Trichinella spp. presence were three times higher in wolverine from southeast than northwest Yukon. The mean intensity of infection was 22.6 ± 39 (SD, range 0.1–295) larvae per gram. Trichinella T6 was the predominant genotype (76%), followed by T. nativa (8%); mixed infections with Trichinella T6 and T. nativa (12%) were observed. In addition, T. spiralis was detected in one wolverine. Out of 22 isolates initially identified as T. nativa in multiplex PCR, 14 were analyzed by PCR-RFLP to distinguish them from T. chanchalensis, a recently discovered cryptic species, which cannot be distinguished from the T. nativa on multiplex PCR. Ten isolates were identified either as T. chanchalensis alone (n = 7), or mixed infection with T. chanchalensis and T. nativa (n = 2) or T. chanchalensis and Trichinella T6 (n = 1)]. Conclusions Wolverine hosted high prevalence, high larval intensity, and multiple species of Trichinella, likely due to their scavenging habits, apex position in the food chain, and wide home range. Wolverine (especially adult males) should be considered as a sentinel species for surveys for Trichinella spp. across their distributional range. Graphical Abstract |
format |
Article in Journal/Newspaper |
author |
Sharma, Rajnish Harms, N. Jane Kukka, Piia M. Jung, Thomas S. Parker, Sarah E. Ross, Sasha Thompson, Peter Rosenthal, Benjamin Hoberg, Eric P. Jenkins, Emily J. |
author_facet |
Sharma, Rajnish Harms, N. Jane Kukka, Piia M. Jung, Thomas S. Parker, Sarah E. Ross, Sasha Thompson, Peter Rosenthal, Benjamin Hoberg, Eric P. Jenkins, Emily J. |
author_sort |
Sharma, Rajnish |
title |
High prevalence, intensity, and genetic diversity of Trichinella spp. in wolverine (Gulo gulo) from Yukon, Canada |
title_short |
High prevalence, intensity, and genetic diversity of Trichinella spp. in wolverine (Gulo gulo) from Yukon, Canada |
title_full |
High prevalence, intensity, and genetic diversity of Trichinella spp. in wolverine (Gulo gulo) from Yukon, Canada |
title_fullStr |
High prevalence, intensity, and genetic diversity of Trichinella spp. in wolverine (Gulo gulo) from Yukon, Canada |
title_full_unstemmed |
High prevalence, intensity, and genetic diversity of Trichinella spp. in wolverine (Gulo gulo) from Yukon, Canada |
title_sort |
high prevalence, intensity, and genetic diversity of trichinella spp. in wolverine (gulo gulo) from yukon, canada |
publisher |
figshare |
publishDate |
2021 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.5331812 https://springernature.figshare.com/collections/High_prevalence_intensity_and_genetic_diversity_of_Trichinella_spp_in_wolverine_Gulo_gulo_from_Yukon_Canada/5331812 |
geographic |
Yukon Canada |
geographic_facet |
Yukon Canada |
genre |
Gulo gulo Yukon |
genre_facet |
Gulo gulo Yukon |
op_relation |
https://dx.doi.org/10.1186/s13071-021-04636-2 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.5331812 https://doi.org/10.1186/s13071-021-04636-2 |
_version_ |
1766022077984276480 |