Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species
Abstract Background Spotted fever group rickettsiae represent one of the most diverse groups of vector-borne bacteria, with several human pathogenic species showing an emerging trend worldwide. Most species are vectored by ticks (Ixodidae), with many zoonotic reservoir species among most terrestrial...
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ftdatacite:10.6084/m9.figshare.c.5301103 2023-05-15T17:48:38+02:00 Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species Matei, Ioana A. Corduneanu, Alexandra Sándor, Attila D. Ionică, Angela Monica Panait, Luciana Kalmár, Zsuzsa Ivan, Talida Papuc, Ionel Bouari, Cosmina Nicodim Fit Mihalca, Andrei Daniel 2021 https://dx.doi.org/10.6084/m9.figshare.c.5301103 https://springernature.figshare.com/collections/Rickettsia_spp_in_bats_of_Romania_high_prevalence_of_Rickettsia_monacensis_in_two_insectivorous_bat_species/5301103 unknown figshare https://dx.doi.org/10.1186/s13071-021-04592-x Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Zoology FOS Biological sciences Collection article 2021 ftdatacite https://doi.org/10.6084/m9.figshare.c.5301103 https://doi.org/10.1186/s13071-021-04592-x 2021-11-05T12:55:41Z Abstract Background Spotted fever group rickettsiae represent one of the most diverse groups of vector-borne bacteria, with several human pathogenic species showing an emerging trend worldwide. Most species are vectored by ticks (Ixodidae), with many zoonotic reservoir species among most terrestrial vertebrate groups. While the reservoir competence of many different vertebrate species is well known (e.g. birds, rodents and dogs), studies on insectivorous bats have been rarely performed despite their high species diversity, ubiquitous urban presence and importance in harboring zoonotic disease agents. Romania has a high diversity and ubiquity of bats. Moreover, seven out of eight SFG rickettsiae species with zoonotic potential were previously reported in Romania. Based on this, the aim of this study was to detect Rickettsia species in tissue samples in bats. Methods Here we report a large-scale study (322 bats belonging to 20 species) on the presence of Rickettsia spp. in Romanian bat species. Tissue samples from insectivorous bats were tested for the presence of Rickettsia DNA using PCR detection amplifying a 381 bp fragment of the gltA gene. Positive results were sequenced to confirm the results. The obtained results were statistically analyzed by chi-squared independence test. Results Positive results were obtained in 14.6% of bat samples. Sequence analysis confirmed the presence of R. monacensis in two bat species (Nyctalus noctula and Pipistrellus pipistrellus) in two locations. Conclusion This study provides the first evidence of a possible involvement of these bat species in the epidemiology of Rickettsia spp., highlighting the importance of bats in natural cycles of these vector-borne pathogens. Article in Journal/Newspaper Nyctalus noctula Pipistrellus pipistrellus DataCite Metadata Store (German National Library of Science and Technology) |
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DataCite Metadata Store (German National Library of Science and Technology) |
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topic |
Zoology FOS Biological sciences |
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Zoology FOS Biological sciences Matei, Ioana A. Corduneanu, Alexandra Sándor, Attila D. Ionică, Angela Monica Panait, Luciana Kalmár, Zsuzsa Ivan, Talida Papuc, Ionel Bouari, Cosmina Nicodim Fit Mihalca, Andrei Daniel Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species |
topic_facet |
Zoology FOS Biological sciences |
description |
Abstract Background Spotted fever group rickettsiae represent one of the most diverse groups of vector-borne bacteria, with several human pathogenic species showing an emerging trend worldwide. Most species are vectored by ticks (Ixodidae), with many zoonotic reservoir species among most terrestrial vertebrate groups. While the reservoir competence of many different vertebrate species is well known (e.g. birds, rodents and dogs), studies on insectivorous bats have been rarely performed despite their high species diversity, ubiquitous urban presence and importance in harboring zoonotic disease agents. Romania has a high diversity and ubiquity of bats. Moreover, seven out of eight SFG rickettsiae species with zoonotic potential were previously reported in Romania. Based on this, the aim of this study was to detect Rickettsia species in tissue samples in bats. Methods Here we report a large-scale study (322 bats belonging to 20 species) on the presence of Rickettsia spp. in Romanian bat species. Tissue samples from insectivorous bats were tested for the presence of Rickettsia DNA using PCR detection amplifying a 381 bp fragment of the gltA gene. Positive results were sequenced to confirm the results. The obtained results were statistically analyzed by chi-squared independence test. Results Positive results were obtained in 14.6% of bat samples. Sequence analysis confirmed the presence of R. monacensis in two bat species (Nyctalus noctula and Pipistrellus pipistrellus) in two locations. Conclusion This study provides the first evidence of a possible involvement of these bat species in the epidemiology of Rickettsia spp., highlighting the importance of bats in natural cycles of these vector-borne pathogens. |
format |
Article in Journal/Newspaper |
author |
Matei, Ioana A. Corduneanu, Alexandra Sándor, Attila D. Ionică, Angela Monica Panait, Luciana Kalmár, Zsuzsa Ivan, Talida Papuc, Ionel Bouari, Cosmina Nicodim Fit Mihalca, Andrei Daniel |
author_facet |
Matei, Ioana A. Corduneanu, Alexandra Sándor, Attila D. Ionică, Angela Monica Panait, Luciana Kalmár, Zsuzsa Ivan, Talida Papuc, Ionel Bouari, Cosmina Nicodim Fit Mihalca, Andrei Daniel |
author_sort |
Matei, Ioana A. |
title |
Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species |
title_short |
Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species |
title_full |
Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species |
title_fullStr |
Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species |
title_full_unstemmed |
Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species |
title_sort |
rickettsia spp. in bats of romania: high prevalence of rickettsia monacensis in two insectivorous bat species |
publisher |
figshare |
publishDate |
2021 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.5301103 https://springernature.figshare.com/collections/Rickettsia_spp_in_bats_of_Romania_high_prevalence_of_Rickettsia_monacensis_in_two_insectivorous_bat_species/5301103 |
genre |
Nyctalus noctula Pipistrellus pipistrellus |
genre_facet |
Nyctalus noctula Pipistrellus pipistrellus |
op_relation |
https://dx.doi.org/10.1186/s13071-021-04592-x |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.5301103 https://doi.org/10.1186/s13071-021-04592-x |
_version_ |
1766154771721355264 |