Development and characterization of thirteen novel microsatellite markers for use in Greenland sharks (Somniosus microcephalus), with cross-amplification in Pacific sleeper sharks (Somniosus pacificus)
Abstract Objective The objectives of this work are to isolate, develop, and characterize polymorphic microsatellite markers for use in Greenland sharks (Somniosus microcephalus). Results Thirteen microsatellite loci were successfully amplified and yielded multi-locus genotypes for 36 S. microcephalu...
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Online Access: | https://dx.doi.org/10.6084/m9.figshare.c.5274305 https://springernature.figshare.com/collections/Development_and_characterization_of_thirteen_novel_microsatellite_markers_for_use_in_Greenland_sharks_Somniosus_microcephalus_with_cross-amplification_in_Pacific_sleeper_sharks_Somniosus_pacificus_/5274305 |
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ftdatacite:10.6084/m9.figshare.c.5274305 2023-05-15T16:27:14+02:00 Development and characterization of thirteen novel microsatellite markers for use in Greenland sharks (Somniosus microcephalus), with cross-amplification in Pacific sleeper sharks (Somniosus pacificus) Swintek, Meaghan A. Walter, Ryan P. 2021 https://dx.doi.org/10.6084/m9.figshare.c.5274305 https://springernature.figshare.com/collections/Development_and_characterization_of_thirteen_novel_microsatellite_markers_for_use_in_Greenland_sharks_Somniosus_microcephalus_with_cross-amplification_in_Pacific_sleeper_sharks_Somniosus_pacificus_/5274305 unknown figshare https://dx.doi.org/10.1186/s13104-021-05447-5 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Genetics FOS Biological sciences Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Sociology FOS Sociology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences Collection article 2021 ftdatacite https://doi.org/10.6084/m9.figshare.c.5274305 https://doi.org/10.1186/s13104-021-05447-5 2021-11-05T12:55:41Z Abstract Objective The objectives of this work are to isolate, develop, and characterize polymorphic microsatellite markers for use in Greenland sharks (Somniosus microcephalus). Results Thirteen microsatellite loci were successfully amplified and yielded multi-locus genotypes for 36 S. microcephalus individuals from Grise Fjord (n = 16) and Svalbard (n = 20). Each locus yielded between 2 and 9 alleles and observed heterozygosity ranged from 0.11 to 0.70 when estimated across both sites. One locus and three loci deviated from HWE following Bonferroni correction, for individuals sampled from Grise Fjord and Svalbard, respectively. Cross-amplification was successful at every locus for five of the ten S. pacificus individuals. Article in Journal/Newspaper Greenland Somniosus microcephalus Svalbard DataCite Metadata Store (German National Library of Science and Technology) Svalbard Greenland Pacific |
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collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Genetics FOS Biological sciences Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Sociology FOS Sociology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences |
spellingShingle |
Genetics FOS Biological sciences Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Sociology FOS Sociology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences Swintek, Meaghan A. Walter, Ryan P. Development and characterization of thirteen novel microsatellite markers for use in Greenland sharks (Somniosus microcephalus), with cross-amplification in Pacific sleeper sharks (Somniosus pacificus) |
topic_facet |
Genetics FOS Biological sciences Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Sociology FOS Sociology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences |
description |
Abstract Objective The objectives of this work are to isolate, develop, and characterize polymorphic microsatellite markers for use in Greenland sharks (Somniosus microcephalus). Results Thirteen microsatellite loci were successfully amplified and yielded multi-locus genotypes for 36 S. microcephalus individuals from Grise Fjord (n = 16) and Svalbard (n = 20). Each locus yielded between 2 and 9 alleles and observed heterozygosity ranged from 0.11 to 0.70 when estimated across both sites. One locus and three loci deviated from HWE following Bonferroni correction, for individuals sampled from Grise Fjord and Svalbard, respectively. Cross-amplification was successful at every locus for five of the ten S. pacificus individuals. |
format |
Article in Journal/Newspaper |
author |
Swintek, Meaghan A. Walter, Ryan P. |
author_facet |
Swintek, Meaghan A. Walter, Ryan P. |
author_sort |
Swintek, Meaghan A. |
title |
Development and characterization of thirteen novel microsatellite markers for use in Greenland sharks (Somniosus microcephalus), with cross-amplification in Pacific sleeper sharks (Somniosus pacificus) |
title_short |
Development and characterization of thirteen novel microsatellite markers for use in Greenland sharks (Somniosus microcephalus), with cross-amplification in Pacific sleeper sharks (Somniosus pacificus) |
title_full |
Development and characterization of thirteen novel microsatellite markers for use in Greenland sharks (Somniosus microcephalus), with cross-amplification in Pacific sleeper sharks (Somniosus pacificus) |
title_fullStr |
Development and characterization of thirteen novel microsatellite markers for use in Greenland sharks (Somniosus microcephalus), with cross-amplification in Pacific sleeper sharks (Somniosus pacificus) |
title_full_unstemmed |
Development and characterization of thirteen novel microsatellite markers for use in Greenland sharks (Somniosus microcephalus), with cross-amplification in Pacific sleeper sharks (Somniosus pacificus) |
title_sort |
development and characterization of thirteen novel microsatellite markers for use in greenland sharks (somniosus microcephalus), with cross-amplification in pacific sleeper sharks (somniosus pacificus) |
publisher |
figshare |
publishDate |
2021 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.5274305 https://springernature.figshare.com/collections/Development_and_characterization_of_thirteen_novel_microsatellite_markers_for_use_in_Greenland_sharks_Somniosus_microcephalus_with_cross-amplification_in_Pacific_sleeper_sharks_Somniosus_pacificus_/5274305 |
geographic |
Svalbard Greenland Pacific |
geographic_facet |
Svalbard Greenland Pacific |
genre |
Greenland Somniosus microcephalus Svalbard |
genre_facet |
Greenland Somniosus microcephalus Svalbard |
op_relation |
https://dx.doi.org/10.1186/s13104-021-05447-5 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.5274305 https://doi.org/10.1186/s13104-021-05447-5 |
_version_ |
1766016347890778112 |