Development and characterization of thirteen novel microsatellite markers for use in Greenland sharks (Somniosus microcephalus), with cross-amplification in Pacific sleeper sharks (Somniosus pacificus)

• Main Authors: Swintek, Meaghan A., Walter, Ryan P.
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: figshare 2021
• Subjects: Genetics; FOS Biological sciences; Evolutionary Biology; 59999 Environmental Sciences not elsewhere classified; FOS Earth and related environmental sciences; Ecology; Sociology; FOS Sociology; 69999 Biological Sciences not elsewhere classified; Inorganic Chemistry; FOS Chemical sciences; Svalbard; Greenland; Pacific; Somniosus microcephalus
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.5274305.v1; https://springernature.figshare.com/collections/Development_and_characterization_of_thirteen_novel_microsatellite_markers_for_use_in_Greenland_sharks_Somniosus_microcephalus_with_cross-amplification_in_Pacific_sleeper_sharks_Somniosus_pacificus_/5274305/1

Abstract Objective The objectives of this work are to isolate, develop, and characterize polymorphic microsatellite markers for use in Greenland sharks (Somniosus microcephalus). Results Thirteen microsatellite loci were successfully amplified and yielded multi-locus genotypes for 36 S. microcephalus individuals from Grise Fjord (n = 16) and Svalbard (n = 20). Each locus yielded between 2 and 9 alleles and observed heterozygosity ranged from 0.11 to 0.70 when estimated across both sites. One locus and three loci deviated from HWE following Bonferroni correction, for individuals sampled from Grise Fjord and Svalbard, respectively. Cross-amplification was successful at every locus for five of the ten S. pacificus individuals.