Temporal proteomic profiling reveals insight into critical developmental processes and temperature-influenced physiological response differences in a bivalve mollusc ...

• Main Authors: Wanamaker, Shelly A., Mitchell, Kaitlyn R., Thompson, Rhonda Elliott, Eudeline, Benoit, Vadopalas, Brent, Timmins-Schiffman, Emma B., Roberts, Steven B.
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: figshare 2024
• Subjects: Ecology; FOS: Biological sciences; Pacific; Crassostrea gigas; Pacific oyster
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.5180070.v2; https://springernature.figshare.com/collections/Temporal_proteomic_profiling_reveals_insight_into_critical_developmental_processes_and_temperature-influenced_physiological_response_differences_in_a_bivalve_mollusc/5180070/2

Abstract Background Protein expression patterns underlie physiological processes and phenotypic differences including those occurring during early development. The Pacific oyster (Crassostrea gigas) undergoes a major phenotypic change in early development from free-swimming larval form to sessile benthic dweller while proliferating in environments with broad temperature ranges. Despite the economic and ecological importance of the species, physiological processes occurring throughout metamorphosis and the impact of temperature on these processes have not yet been mapped out. Results Towards this, we comprehensively characterized protein abundance patterns for 7978 proteins throughout metamorphosis in the Pacific oyster at different temperature regimes. We used a multi-statistical approach including principal component analysis, ANOVA-simultaneous component analysis, and hierarchical clustering coupled with functional enrichment analysis to characterize these data. We identified distinct sets of proteins with ...