Supplementary material from " Amphibalanus amphitrite begins exoskeleton mineralization within 48 hours of metamorphosis"
Barnacles are ancient arthropods that, as adults, are surrounded by a hard, mineralized, outer shell that the organism produces for protection. While extensive research has been conducted on the glue-like cement that barnacles use to adhere to surfaces, less is known about the barnacle exoskeleton,...
Main Authors: | , , , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
The Royal Society
2020
|
Subjects: | |
Online Access: | https://dx.doi.org/10.6084/m9.figshare.c.5127055 https://rs.figshare.com/collections/Supplementary_material_from_i_Amphibalanus_amphitrite_i_begins_exoskeleton_mineralization_within_48_hours_of_metamorphosis_/5127055 |
id |
ftdatacite:10.6084/m9.figshare.c.5127055 |
---|---|
record_format |
openpolar |
spelling |
ftdatacite:10.6084/m9.figshare.c.5127055 2023-05-15T17:51:23+02:00 Supplementary material from " Amphibalanus amphitrite begins exoskeleton mineralization within 48 hours of metamorphosis" Metzler, Rebecca A. O'Malley, Jessica Herrick, Jack Christensen, Brett Orihuela, Beatriz Rittschof, Daniel Dickinson, Gary H. 2020 https://dx.doi.org/10.6084/m9.figshare.c.5127055 https://rs.figshare.com/collections/Supplementary_material_from_i_Amphibalanus_amphitrite_i_begins_exoskeleton_mineralization_within_48_hours_of_metamorphosis_/5127055 unknown The Royal Society https://dx.doi.org/10.1098/rsos.200725 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Developmental Biology 90301 Biomaterials FOS Medical engineering Collection article 2020 ftdatacite https://doi.org/10.6084/m9.figshare.c.5127055 https://doi.org/10.1098/rsos.200725 2021-11-05T12:55:41Z Barnacles are ancient arthropods that, as adults, are surrounded by a hard, mineralized, outer shell that the organism produces for protection. While extensive research has been conducted on the glue-like cement that barnacles use to adhere to surfaces, less is known about the barnacle exoskeleton, especially the process by which the barnacle exoskeleton is formed. Here, we present data exploring the changes that occur as the barnacle cyprid undergoes metamorphosis to become a sessile juvenile with a mineralized exoskeleton. Scanning electron microscope (SEM) data show dramatic morphological changes in the barnacle exoskeleton following metamorphosis. Energy-dispersive x-ray spectroscopy (EDS) indicates a small amount of calcium (8%) 1 h post-metamorphosis that steadily increases to 28% by 2 days following metamorphosis. Raman spectroscopy indicates calcite in the exoskeleton of a barnacle 2 days following metamorphosis and no detectable calcium carbonate in exoskeletons up to 3 h post-metamorphosis. Confocal microscopy indicates during this 2-day period, barnacle base plate area and height increases rapidly (0.001 mm 2 hr −1 and 0.30 µm hr −1 , respectively). These results provide critical information into the early life stages of the barnacle, which will be important for developing an understanding of how ocean acidification might impact the calcification process of the barnacle exoskeleton. Article in Journal/Newspaper Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Developmental Biology 90301 Biomaterials FOS Medical engineering |
spellingShingle |
Developmental Biology 90301 Biomaterials FOS Medical engineering Metzler, Rebecca A. O'Malley, Jessica Herrick, Jack Christensen, Brett Orihuela, Beatriz Rittschof, Daniel Dickinson, Gary H. Supplementary material from " Amphibalanus amphitrite begins exoskeleton mineralization within 48 hours of metamorphosis" |
topic_facet |
Developmental Biology 90301 Biomaterials FOS Medical engineering |
description |
Barnacles are ancient arthropods that, as adults, are surrounded by a hard, mineralized, outer shell that the organism produces for protection. While extensive research has been conducted on the glue-like cement that barnacles use to adhere to surfaces, less is known about the barnacle exoskeleton, especially the process by which the barnacle exoskeleton is formed. Here, we present data exploring the changes that occur as the barnacle cyprid undergoes metamorphosis to become a sessile juvenile with a mineralized exoskeleton. Scanning electron microscope (SEM) data show dramatic morphological changes in the barnacle exoskeleton following metamorphosis. Energy-dispersive x-ray spectroscopy (EDS) indicates a small amount of calcium (8%) 1 h post-metamorphosis that steadily increases to 28% by 2 days following metamorphosis. Raman spectroscopy indicates calcite in the exoskeleton of a barnacle 2 days following metamorphosis and no detectable calcium carbonate in exoskeletons up to 3 h post-metamorphosis. Confocal microscopy indicates during this 2-day period, barnacle base plate area and height increases rapidly (0.001 mm 2 hr −1 and 0.30 µm hr −1 , respectively). These results provide critical information into the early life stages of the barnacle, which will be important for developing an understanding of how ocean acidification might impact the calcification process of the barnacle exoskeleton. |
format |
Article in Journal/Newspaper |
author |
Metzler, Rebecca A. O'Malley, Jessica Herrick, Jack Christensen, Brett Orihuela, Beatriz Rittschof, Daniel Dickinson, Gary H. |
author_facet |
Metzler, Rebecca A. O'Malley, Jessica Herrick, Jack Christensen, Brett Orihuela, Beatriz Rittschof, Daniel Dickinson, Gary H. |
author_sort |
Metzler, Rebecca A. |
title |
Supplementary material from " Amphibalanus amphitrite begins exoskeleton mineralization within 48 hours of metamorphosis" |
title_short |
Supplementary material from " Amphibalanus amphitrite begins exoskeleton mineralization within 48 hours of metamorphosis" |
title_full |
Supplementary material from " Amphibalanus amphitrite begins exoskeleton mineralization within 48 hours of metamorphosis" |
title_fullStr |
Supplementary material from " Amphibalanus amphitrite begins exoskeleton mineralization within 48 hours of metamorphosis" |
title_full_unstemmed |
Supplementary material from " Amphibalanus amphitrite begins exoskeleton mineralization within 48 hours of metamorphosis" |
title_sort |
supplementary material from " amphibalanus amphitrite begins exoskeleton mineralization within 48 hours of metamorphosis" |
publisher |
The Royal Society |
publishDate |
2020 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.5127055 https://rs.figshare.com/collections/Supplementary_material_from_i_Amphibalanus_amphitrite_i_begins_exoskeleton_mineralization_within_48_hours_of_metamorphosis_/5127055 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://dx.doi.org/10.1098/rsos.200725 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.5127055 https://doi.org/10.1098/rsos.200725 |
_version_ |
1766158522735656960 |